MicroRNA extraction data for deep sequencing group
Deep Sequencing the MicroRNA Transcriptome in Colorectal Cancer
... and sequencing were found for miR-101 and ...the sequencing were both able to detect but not to separate between known isoforms of these ...the sequencing data, no nucleotide variations were ...
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miRDis: a Web tool for endogenous and exogenous microRNA discovery based on deep-sequencing data analysis
... miRNA sequencing data analysis including miRDeep2 [ 14 ], CAP-miRSeq [ 15 ], DSAP [ 16 ], DARIO [ 17 ], omiRas [ 18 ], sRNAbench [ 19 ], ShortStack [ 20 ], miRDeep-P [ 21 ] and miR- PREFeR [ 22 ], where the ...
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Analysis of microRNA profile of Anopheles sinensis by deep sequencing and bioinformatic approaches
... Results: We identified 49 conserved miRNAs and 12 novel miRNAs by next-generation high-throughput sequencing technology. In contrast, 43 miRNAs were predicted by the bioinformatic approach, of which two were ...
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Integrated analysis of microRNA regulatory network in nasopharyngeal carcinoma with deep sequencing
... Four softwares (RNAhybrid, TargetScan, Miranda, PITA) were used for target gene prediction and only the genes identified by all four approaches were selected out [22–25]. And we chose the overlapped genes targeted by ...
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Discovery of novel human breast cancer micrornas from deep sequencing data by analysis of pri-microrna secondary structures
... Using deep sequencing technology, we have identified 189 candidate novel microRNAs in human breast cancer cell lines with diverse tumorigenic ...from deep sequencing data with ...
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Identification and Analysis of the Porcine MicroRNA in Porcine Cytomegalovirus-Infected Macrophages Using Deep Sequencing
... geneontology.org) online server was used to provide a controlled vocabulary of terms for describing gene product characteristics and gene product annotation data from GO consor- tium members. We used Fisher ’s ...
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De novo discovery of microRNA from small RNA sequencing data
... Sanger sequencing consists in four separated reactions in which a DNA template, DNA primer and a polymerase are mixed together with a pool of dNTP — deoxynucleosidetriphosphates or DNA bases: dATP, dGTP, dTTP and ...
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MicroRNA involvement in mechanism of endogenous protection induced by fastigial nucleus stimulation based on deep sequencing and bioinformatics
... The miRNA microarray assays were performed by a ser- vice provider (LC Sciences, Houston, USA). The cus- tomμparaflo™ microfluidic chip contained 728 unique miRNAs listed in Sanger miRBase release 20.0 and 479 predicted ...
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Accurate Viral Population Assembly From Ultra-Deep Sequencing Data
... high-fidelity sequencing protocol (Kinde et ...High-fidelity sequencing protocol, known as Safe- SeqS, has been applied to detect rare somatic mutations, but its application on detecting rare viral ...
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Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015.
... 1 Division of Biomedical & Life Sciences, Faculty of Health & Medicine, Lancaster University, Lancaster LA 1 4YT, UK. 2 Royal Lancaster In firmary, Ashton Road, Lancaster LA1 4RP, UK. 3 Centre for Ecology & ...
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Novel MicroRNA Involved in Host Response to Avian Pathogenic Escherichia coli Identified by Deep Sequencing and Integration Analysis
... CM group compared to the level in the NC group and may inhibit inflammation by enhancing CTSG, which has broad-spectrum antibiotic properties against Staphylococcus aureus, Klebsiella pneumonia and ...
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A novel qualitative approach to analyse DNA methylation data from Deep Bisulfite Amplicon Sequencing (Deep- Bis)
... DNA sequencing technology, the so-called Next Generation Sequencing (NGS) technology, enables researchers to obtain many thousands of sequence reads in a single sequencing run and at a cost that is ...
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Deep sequencing of the vaginal microbiota of women with HIV
... DNA extraction and amplification Swabs were vigorously agitated in 1 mL of PBS (pH ...After extraction, 10 m l of the Instagene supernatant was used as template for a 50 m l PCR ...Illumina ...
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Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge \u3ci\u3eMayetiola destructor\u3c/i\u3e
... The first instar larva is the life stage of Hessian fly that determines the outcome of an infestation, resulting in the host plant becoming resistant or susceptible [25]. To identify miRNAs likely to be involved in ...
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Deep Sequencing Data Analysis
... in sequencing to answer their experimental questions should prepare themselves to join a fast-moving field and embrace the tools being developed specifically for ...
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miRTrace reveals the organismal origins of microRNA sequencing data
... quencing data back to 14 animal and plant clades with high (> 99%) ...sequencing data. We have analyzed more than 700 public miRNA sequencing datasets and find evidence that more than 7% have ...
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Transcript identification from deep sequencing data
... simulated data for the human genome as well as on an extensive RNA-Seq data set for the model organism Drosophila melanogaster we show that MiTie predicts transcripts significantly more accurate than ...
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Deep Sequencing Data Analysis: Challenges and Solutions
... of data produced by a single ultra high throughput sequencing run is often tremendous and can reach hundreds of millions of reads in various lengths per experiment (Mardis, ...of data is a ...
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Interpreting viral deep sequencing data with GLUE
... raw deep sequencing reads into Sequence Alignment Mapping (SAM) ...a deep sequencing dataset obtained from a cohort of 241 UK HCV patients for whom prior treatment with direct-acting ...
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Deep Web Data Extraction by Using Vision-Based Item and Data Extraction Algorithms
... especially deep WebPages, the underlying structure of current Web pages is More complicated than ever and is far different from their Layouts on Web ...of deep Web pages always arrange the data ...
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