minimal essential medium (α modified)
Effect of various concentrations of Minimal Essential Medium vitamins (MEM vitamins) on development of sheep oocytes during in-vitro maturation
... The medium used was SOF, as originally described by Tervit et al (14), enriched with 10% heat inactivated estrous sheep serum. Sperms were counted by a hemacytometer and checked for acceptable motility (i.e., at ...
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The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K /Akt pathway
... Eagle’s minimal essential medium (EMEM), fetal bo- vine serum (FBS), penicillin, streptomycin, L-glutamine, carboxy-H2DCFDA and oxidized carboxy-DCFDA were from Invitrogen (Carlsbad, CA, ...
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Production of cells and viruses in a new multiple tube tissue culture propagator
... Rubella virus strain M33 was grown in Vero cells cultivated in equal parts of medium 199 Hanks base and minimal essential medium Earle base supplemented with 4% kaolintreated calf serum [r] ...
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Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells
... culture medium differs with respect to serum and glucose con- centration, as well as in supplementation of ascorbic acid, growth factors and non-essential amino ...alpha-Minimal Essential ...
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Analysis of Immunoprecipitated Surface Glycoproteins in Measles Virions and in Membranes of Infected Cells
... Vero cells in roller bottles were washed with Dulbecco phosphate-buffered saline, pH 7.2 PBS, and inoculated with measles virus LEC strain in minimal essential medium without serum at 29[r] ...
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Plaque assay of Sendai virus in monolayers of a clonal line of porcine kidney cells
... The growth medium was then removed and, without washing the cell sheet, serial dilutions of virus prepared in bicarbonate-free minimal essential medium supplemented with 10% tryptose pho[r] ...
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C3H/HeN mammary tumor-bearing mice develop type-specific neutralizing antibodies and group-specific precipitating antibodies for the mouse mammary tumor virus.
... Fischer rat embryo FRE cels were propagated in Eagle-modified minimal essential medium with Earle salts EMEM containing 5% fetal 123 Downloaded from http://jvi.asm.org/ on November 10, 2[r] ...
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Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis
... phosphatase; α -MEM: minimal essential medium, α -modification; ANOVA: analysis of variance; BMP: bone morphogenic protein; BSA: bovine serum albumin; BS/BV: bone surface/volume ratio; ...
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Immunoglobulin G(Fc)-binding receptors on virions of herpes simplex virus type 1 and transfer of these receptors to the cell surface by infection.
... 2 HEp-2 and African green monkey kidney Vero cells were grown in monolayer cultures in Dulbecco-modified Eagle minimal essential medium sup512 Downloaded from http://jvi.asm.org/ on Nove[r] ...
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Method of examining viral RNA metabolism in cells in culture: metabolism of vesicular stomatitis virus RNA.
... At the end of the desired labeling period, the fluids containing the [3H]uridine were removed, and the cultures were washed two times with minimal essential medium or minimal essential m[r] ...
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Impaired osteogenesis in Menkes disease-derived induced pluripotent stem cells
... The cells were fixed with 4 % formaldehyde for 30 min, washed twice in PBST (PBS containing 0.1 % Tween 20), and permeabilized in PBS containing 0.1 % Triton X-100 (Sigma) for 20 min. After blocking with 2 % bovine serum ...
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Follicle-stimulating hormone responsiveness in antral follicles from aryl hydrocarbon receptor knockout mice
... knockout; α -MEM: Alpha-minimal essential media; ANOVA: Analysis of variance; CYP11A1: Cytochrome P450, family 11, subfamily A, polypeptide 1; CYP17A1: Cytochrome P450, family 17, subfamily A, ...
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Validation of Novaprep® HQ+ liquid-based cytology medium for high-risk human papillomavirus detection by hc2
... Methods: Repeatability, reproducibility, method comparison and stability (-20 °C, +4 °C, +20 °C and +40 °C up to six months) were evaluated from HPV16 and HPV18 positive cell lines diluted in the Novaprep® HQ+ ...
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CCAAT/enhancer binding protein β promotes receptor activator of nuclear factor kappa B ligand (RANKL) expression and osteoclast formation in the synovium in rheumatoid arthritis
... -MEM: α -minimum essential medium; AP-1: activator protein-1; ATF-4: activation transcription factor 4; bp: base pairs; C/EBP: CCAAT/enhancer binding protein; ChIP: chromatin immunoprecipitation; ...
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Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation
... Current innovative approaches for articular cartilage repair are functional tissue engineering strategies com- bined mesenchymal stem cells (MSCs), biomimetic scaf- fold, growth factors, and mechanical stimuli [8, 9]. ...
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Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration
... Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F-12 (Gluta- MAX; Life Technologies, Carlsbad, CA, USA) + 2% penicillin/streptomycin (P/S; PAA Laboratories, Cölbe, ...culture medium and ...
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Intralesional injection of adipose-derived stem cells reduces hypertrophic scarring in a rabbit ear model
... Scar tissues were snap-frozen in liquid nitrogen imme- diately after harvest. Before total RNA could be ob- tained, the scar samples were homogenized with an electronic high-speed homogenizer (Scientz, Zhejiang, China). ...
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Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation
... were gently applied onto the Matrigel® thick gel layer and cultured in EGM-2 medium. Sprouting was evalu- ated after 24 h (sets of three spheroids) and after 7 days (sets of three spheroids) by measuring the ...
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Native extracellular matrix preserves mesenchymal stem cell “stemness” and differentiation potential under serum-free culture conditions
... “expansion medium” containing alpha-minimum essential medium (α-MEM) (Life Tech- nologies, Grand Island, NY, USA), glutamine (2 mM), penicillin (100 U/ml), streptomycin (100 μg/ml; Biofluids, ...
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Preconditioning of mesenchymal stem cells for improved transplantation efficacy in recessive dystrophic epidermolysis bullosa
... of α-smooth muscle actin [44], a marker at- tributed to but not specific for ...increased α-smooth muscle actin and associated rates of contractile activity are not limited to fibrotic processes, and in ...
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