miR-15a
miR-15a and miR-16-1 inhibit the proliferation of leukemic cells by down-regulating WT1 protein level
... The 3’UTR segments from the WT1 and Bcl-2 gene were amplified by PCR from cDNA and inserted into the pGL3 control vector (Promega), using the Xba Ι site immediately downstream from the stop codon of luciferase. Bcl-2 is ...
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miR-15a-3p and miR-16-1-3p Negatively Regulate Twist1 to Repress Gastric Cancer Cell Invasion and Metastasis
... of miR-15a-3p and ...of miR-15a-3p and miR-16-1-3p recognition elements on the human Twist1-3’ ...increased miR-15a-3p and miR-16-1-3p expression, ...using ...
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Decreased miR-155-5p, miR-15a, and miR-186 Expression in Gastric Cancer Is Associated with Advanced Tumor Grade and Metastasis
... of miR-15a was significantly lower in GC samples compared to NG subjects [27] .... MiR-15a/16-1 gene cluster locates in chromosome 13 of the human genome and mainly acts as a tumor suppressor ...
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Role of mir-15a/16-1 in early B cell development in a mouse model of chronic lymphocytic leukemia
... of miR-15a in differentiated NZB ES cells followed the expression of Pax5, ...of miR-150 in NZB ES cells (Figure 5F), which was used as a maturation marker in this ...in miR levels were ...
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Fatty acid synthase is a primary target of MiR-15a and MiR-16-1 in breast cancer
... the miR- 15a-16-1 and miR-497-195 miRNAs (Figure 2A), and control constructs expressing mutated ...of miR-15a-16-1 or miR-497-195 ...of miR-Cont, ...
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IL-10Rα expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma
... combination, affected the transcription of the pGL3-IL- 10Rα-mut vector (Fig. 3b). When blocking the expression of the three miRNAs with specific inhibitors, luciferase in- tensity markedly increased in cells transfected ...
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Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p
... with miR-15a-5p and WT-PVT1 compared with that in 293T cells transfected with WT- PVT1 and miR-NC, while the luciferase activity did not change with the transfection of miR-15a-5p and ...
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FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression
... only miR-15a-5p and miR-15b-5p were upregulated significantly in CC tissues ...of miR-15a/b-5p were decreased by the upregulated FENDRR ...of miR- ...that ...
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LncRNA LUADT1 sponges miR-15a-3p to upregulate Twist1 in small cell lung cancer
... Levels of LUADT1 in two types of tissues (SCLC and non-tumor) were measured and compared by qPCR and paired t-test, respectively. Comparing to non-tumor sam- ples, significantly higher expression levels of LUADT1 were ...
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Vitamin D manipulates miR-181c, miR-20b and miR-15a in human umbilical vein endothelial cells exposed to a diabetic-like environment
... (miR-510, miR- 15a, miR-20b, miR-126, and ...miR-181C). miR-659 and miR-411 were validated because in the microarray ana- lysis they were significantly changed ...
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LncRNA KCNQ1OT1 sponges miR-15a to promote immune evasion and malignant progression of prostate cancer via up-regulating PD-L1
... Cancer development is a multistep process involving the acquirement of eight essential hallmarks “sustaining pro- liferative signaling, evading growth suppression, resist- ing cell death, enabling replicative ...
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Suv39h1 promotes facet joint chondrocyte proliferation by targeting miR-15a/Bcl2 in idiopathic scoliosis patients
... Bcl2-targeted miR-15a was downregulated in IS patients, and the level of H3K9me3 in the promoter region of the miR-15a host gene was remarkably increased in IS patients compared with the ...
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Dysregulation of miR-15a and miR-214 in human pancreatic cancer
... both miR-15a and miR-214 in pancreatic cancer cells, we predicted and validated potential targets for both ...of miR-15a chosen for experimental validation were PIM1, CDC25A, BCL2L2, ...
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Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells
... of miR-15a/16-1 could deduce WT1 expression but downregulation of WT1 by siRNA could not increase the expression of miR-15a/16-1 in leukemic cells Our previous data showed overexpression of ...
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Reduction of gastric cancer proliferation and invasion by miR- 15a mediated suppression of Bmi-1 translation
... our observation that knock-down Bmi-1 expression by miR-15a reduces gastric cancer cell proliferation (Figure 4A–4B). Our results suggest that there is a good possibility that a relative large number of ...
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miR-15a/16 reduces retinal leukostasis through decreased pro-inflammatory signaling
... Total RNA was isolated and purified using the TRIzol method, and the purity and quantity of RNA were mea- sured using Synergy HTX multi-mode reader (BioTek; Winooski, VT). For polyA tailing reverse-transcriptase PCR, 5 ...
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MiR 15a/ 16 inhibit angiogenesis by targeting Tie2 coding sequence : therapeutic potential of a miR 15a/16 decoy system in limb ischemia
... of miR-15a/16 Improves Blood Flow (BF) Recovery, Post-ischemic Neovascularization, and Toe Survival following Limb Ischemia Limb ischemia was induced in mice by femoral artery ligation, and ...
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Computational Identification of microRNA 15a predicted to regulate Brain Derived Neurotrophic Factor involved in Type 2 Diabetic Retinopathy
... It was concluded that the sequence retrieval of hsa-miR-15a-5p was the first and most important step for further sequence analysis. In miR-15a annotation, sequence retrieval helped identify ...
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Hepatitis B Virus Regulates Apoptosis and Tumorigenesis through the MicroRNA-15a-Smad7-Transforming Growth Factor Beta Pathway
... of miR-15a to regulate TGF-  ...as miR-15a sponges and increase the expression level of Bcl-2, which is one of the miR-15a ...of miR-15a. HBV mRNAs reduced the ...
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Availability of Circulating MicroRNAs as a Biomarker for Early Diagnosis of Diffuse Large B Cell Lymphoma
... hsa- miR-21, hsa-miR-155, hsa-miR-181a, and hsa-miR-210) were selected as candidate biomarkers (Table ...as miR-16, miR-39, miR-24, RNU6B, and RNU48, have previously been ...
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