miRNA mimics
MicroRNAs signatures, bioinformatics analysis of miRNAs, miRNA mimics and antagonists, and miRNA therapeutics in osteosarcoma
... various algorithms and tools such as miRDeep (miRD- eep/miRDeep2) and miRanalyzer [25, 34, 35] by which miRNA characteristics such as sequence conservation, structural properties (i.e., hairpin and minimum free ...
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The Function of miR 31 Expression in Differentiation of Neural Stem Cells
... Individual miRNA can be inhibited multiple target genes simulta- neously to regulated complex physiological and pathological ...development, miRNA Mimics and miRNA Mimics control are ...
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IL-10Rα expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma
... combination, affected the transcription of the pGL3-IL- 10Rα-mut vector (Fig. 3b). When blocking the expression of the three miRNAs with specific inhibitors, luciferase in- tensity markedly increased in cells transfected ...
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Identification of miR-30b-3p and miR-30d-5p as direct regulators of androgen receptor signaling in prostate cancer by complementary functional microRNA library screening
... 810 miRNA mimics to identify miRNAs that alter AR activity in complementary functional assays including protein lysate microarray (LMA) quantification of AR and PSA protein levels, AR transcriptional ...
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Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression
... miRNA mimics, siRNAs, NC mimics, NC siRNA, miR-137 antagomiR and antagomiR-NC were purchased from Dharmacon (GE Dharmacon, Lafayette, CO, United ...(50nM) miRNA mimics, siRNAs or ...
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Up-regulation of matrix metalloproteinases in a mouse model of chemically induced colitis-associated cancer: the role of microRNAs
... seven miRNA mimics (or miRNA inhibitors) into the murine colon cancer cell line ...miR-330 mimics reduced the levels of Mmp3, Mmp10, and Mmp13 mRNAs, respectively, whereas each of the ...
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Erratum to Hypoxia induced down regulation of microRNA 449a/b impairs control over targeted SERPINE1 (PAI 1) mRNA a mechanism involved in SERPINE1 (PAI 1) overexpression
... either miRNA-449a/b inhibitors or mimics, we noted the expected decrease or increase of the miRNA-449 subtype under investigation (Figure five) (figure 3 here), an effect only investigated under ...
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MiRNAs in cancer therapy: focusing on their bi-directional roles
... Meanwhile, miRNA-based therapy offers some advantages over other gene engineering strategies and protein-target-based immune modulation, for example, miRNAs could target multiple molecules simultaneously, and ...
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Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas
... well) were plated in triplicate in 24-well tissue culture plates. The next day, cells were transfected with the following: Psicheck™-2 plasmid, either wild type CHD5 3’-UTR, or mutated CHD5 3’-UTR miRNA along with ...
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Circular RNA CircEZH2 Suppresses Transmissible Gastroenteritis Coronavirus-induced Opening of Mitochondrial Permeability Transition Pore via Targeting MiR-22 in IPEC-J2
... miR-22 mimics (or miRNA mimics control, miR-22 inhibitor, miRNA inhibitor control) were co-transfected into IPEC-J2 ...miR-22 mimics and inhibitor did not affect psi-IL-6-Mut reporter ...
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Hsa-miR-574-5p negatively regulates MACC-1 expression to suppress colorectal cancer liver metastasis
... that miRNA could bind to the 3′UTR but not the mutant 3′ ...and miRNA mimics transfection on MACC-1 ex- pression in SW1116 and HCT116 cell lines showed that hsa-miR-574-5p negatively regulated MACC-1 ...
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Epigenetic inhibition of miR-663b by long non-coding RNA HOTAIR promotes pancreatic cancer cell proliferation via up- regulation of insulin-like growth factor 2
... miR-663b mimics transfection in cells with MUT IGF2 3’UTR reporter (Figure ...miR-663b mimics transfection significantly reduced the mRNA and protein expression levels of IGF2 in Panc-1 and ...scramble ...
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<p>miR-214-3p Regulates Multi-Drug Resistance and Apoptosis in Retinoblastoma Cells by Targeting ABCB1 and XIAP</p>
... Figure 4 ABCB1 and XIAP are direct target genes of miR-214-3p. (A) Predicted binding sites of miR-214-3p in the 3’UTR of ABCB1 and XIAP analyzed using TargetScan 7.2. (B) Protein expression level of ABCB1 and XIAP in ...
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<p>LncRNA PVT1 Enhances Proliferation and Cisplatin Resistance via Regulating miR-194-5p/HIF1a Axis in Oral Squamous Cell Carcinoma</p>
... candidate miRNA that could bind to PVT1 and HIF1a 3 ʹ UTR (Figure ...miR-194-5p mimics decreased the luciferase activity of WT vectors, but not Mut vectors (Figure 4B and ...miR-194-5p mimics only ...
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Original Article Expression of miR-34a and its role in human papillary thyroid carcinoma
... miR-34a mimics and scrambled miRNA was set as negative ...scrambled miRNA, while the percentage was ...miR-34a mimics (Figure 3A and ...scrambled miRNA, while the percentage was ...
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Recent progress in microRNA-based delivery systems for the treatment of human disease
... targets miRNA-122; it is conjugated to a multivalent N-acetylgalactosamine carbohydrate structure that was de- signed to enhance uptake via binding to the asialoglycopro- tein receptor on ...liposome-encapsulated ...
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The potential role of miRNAs in therapy of breast and ovarian cancers associated with BRCA1 mutation
... how miRNA-638 influenced sensi- tivity to UV-light and cisplatin in triple- negative breast cancer ...that miRNA-638 regulates BRCA1 ...of miRNA-638 can lead to an increased susceptibility of TNBC ...
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MicroRNAs AS BIOMARKERS FOR ACUTE MYOCARDIAL INFARCTION - SMALL MOLECULES WITH A HUGE POTENTIAL
... MiRNAs are small, evolutionary conserved small ribonucleic acid molecules (17-25 nucleotides in length) which have recently become the focus of inten- sive research effort among the different noncoding RNA variants (3, ...
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<p>MicroRNAs and Risk Factors for Diabetic Nephropathy in Egyptian Children and Adolescents with Type 1 Diabetes</p>
... of miRNA-377, miRNA-93, miRNA-25, miRNA-216a, and miRNA-21, in a sample of type 1 diabetic children and adolescents to explore their association with risk factors and other indices of ...
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MicroRNA-155 is a potential molecular marker of natural killer/T-cell lymphoma
... (miRNA-34a, miRNA-34b, miRNA-34c, miRNA-155, miRNA-21, miRNA-221), which were expressed at high, intermediate, and low levels in samples from the NKTCL cell lines SNK-6 and YKS, ...
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