Mirna profiling
Evaluation of a new high-dimensional miRNA profiling platform
... filing system has been described previously, showing the accuracy of the platform by rt-PCR and digital sequencing [21] using RNA from four cell lines (the same cell lines were used in this report) and purchased human ...
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miRNA profiling of NurOwn®: mesenchymal stem cells secreting neurotrophic factors
... a miRNA was below the level of detection for the arrays, a nominal intensity value is given to these data points to avoid errors arising from non-computable mathematical operations during subsequent data ...
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Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma
... example, miRNA-125b expression is decreased in hepatocellular carcinoma (HCC) tissues and HCC cell lines ...leukemia, miRNA-125b levels are elevated, implying that it functions as an oncogene in these ...
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Candidate microRNA biomarkers in human epithelial ovarian cancer: systematic review profiling studies and experimental validation
... were miRNA expression profiling studies in ovarian cancer patients or ovarian cancer cell lines; ② they used tissue samples obtained from surgically removed EOC specimens or epithelial ovarian cancer cell ...
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Challenges and standardization of microRNA profiling in serum and cerebrospinal fluid in dogs suffering from non-infectious inflammatory CNS disease
... of miRNA levels between cases and ...recent miRNA investigations of canine CNS ...circulating miRNA profiling, including pre‑, post‑ and analytical methods based on our experience, in order to ...
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Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes
... vivo miRNA profiling, it is the combinatorial nature of miRNA action in vivo that seems to be most ...the miRNA field, namely that of interpretation of biological consequences of in vivo ...
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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
... circulating miRNA biomarkers by qRT-PCR profiling that is composed of four steps: (1) sample collection and preparation; (2) global miRNA profiling using qRT-PCR; (3) data normalization and ...
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Microarray analysis of MicroRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1)
... comprehensive miRNA profiling in peripheral blood mononuclear cells (PBMCs) from critically ill patients with swine-origin influenza pandemic H1N1 (2009) virus infection via miRNA microarray and ...
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miR-151-5p, targeting chromatin remodeler SMARCA5, as a marker for the BRCAness phenotype
... RNA profiling and BRCA1 promoter methylation analyses could be able to characterize familial non-BRCA1/2 tumors and to discriminate BRCA1-like BCs displaying the so-called “BRCAness” phenotype, intending with this ...
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miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation specific miRNAs
... between miRNA profiling platforms (unpublished observations), and our study used LNA™ technology-based microarrays to quantify miRNA expression in the cell lines whereas Cimino and collea- gues used ...
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Integration of microRNA signatures of distinct mammary epithelial cell types with their gene expression and epigenetic portraits
... and miRNA profiling of 101 pri- mary breast tumors revealed differential expression of miRNAs between these different subgroups ...large-scale miRNA profiling of breast cancers carried out ...
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Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
... global miRNA profiling describes, for the first time, the miRNA expression profile in the human endometrium dur- ing the luteal phase following COS for IVF and luteal sup- port with steroid ...
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MicroRNA 330 5p as a putative modulator of neoadjuvant chemoradiotherapy sensitivity in oesophageal adenocarcinoma
... specimens to predict pCR in patients who received neo-CRT. The likelihood of pCR increased in a near-linear relationship with the MEP score, and identified groups of patients with high probability ( 80%) and low ...
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Micro-RNAs as Potential New Molecular Biomarkers in Oncology: Have They Reached Relevance for the Clinical Imaging Sciences?
... Minimally invasive biomarkers for early cancer detection and monitoring of personalized therapies are of high importance to further improve prognosis in oncological disease. MicroRNAs (miRNAs) are small regulatory RNAs ...
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miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors
... To identify mRNA transcripts that can be potentially regulated by miR-411, mRNA profiles generated using the same total RNA samples used for miRNA profiling were analyzed to identify potential mRNA targets ...
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MicroRNA and mRNA expression profiling in rat acute respiratory distress syndrome
... The miRNA profiling was used to identify the miRNAs involved in the pathogenesis of various lung diseases such as ventilator-induced lung injury [15], bronchopulmonary dysplasia (BPD) [16,17], chronic ...
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Dynamic Regulation of miRNA Expression by Functionally Enhanced
... miRNA profiling of invaded naïve placenta-derived mesenchymal stem cells (PD-MSCs). 433[r] ...
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A contrasting function for miR-137 in embryonic mammogenesis and adult breast carcinogenesis
... mammary gland region) at E13.5 was dephosphorylated with ~15 Units of calf intestine alkaline phosphatase (CIP), followed by RNA denaturation with ~40% DMSO and a 10 min incubation at 100 °C. Dephosphorylated RNA was ...
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Expression profiling of miRNA-145 and miRNA-338 in serum and sputum of patients with COPD, asthma, and asthma–COPD overlap syndrome phenotype
... that miRNA could be important in obstructive diseases, because it may modulate the expres- sion of different genes responsible for many biological pro- cesses such as development of airway epithelial cells, stress ...
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Tumor heterogeneity in the recurrence of epithelial ovarian cancer demonstrated by polycomb group proteins
... GAG CAG AAG CAG GGA G-3 ′ ; reverse primer, 5 ′ -GGT GTC GTG GAG TCG-3 ′ . Primers for miR-4261 were as follows: forward primer, 5 ′ -ACA CTC AGC TGG GAG GAA ACA GGG ACC C-3 ′ ; reverse primer 5 ′ -GGT GTC GTG GAG TCG-3 ...
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