molecular clone
Construction of a molecular clone of ovine enzootic nasal tumor virus
... ENTV-1 molecular clone was generated from two large overlapping PCR products, we investigated whether mutations might have been acquired during the amplification step, which could have affected protease ac- ...
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Molecular and biological characterization of a neurovirulent molecular clone of simian immunodeficiency virus.
... the molecular determinants of neurovirulence, we constructed an infectious simian immunode- ficiency virus (SIV) molecular clone, SIV/17E-Fr, that contained the 3 * end of a neurovirulent strain of ...
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Construction and Characterization of an Infectious Molecular Clone of Koala Retrovirus
... Construction of an infectious molecular clone of strain Aki. The genomic DNA of HEK293T cells infected with KoRV strain Aki was iso- lated by using a QIAamp DNA Blood Kit (Qiagen, Valencia, CA). We ...
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Characterization of an infectious molecular clone of human T-cell leukemia virus type I.
... a molecular clone of HTLV-I was derived from RH/K30 (K30 f ) and subcloned into a plasmid vector (K30p) ...this clone appears to encode all of the known structural, enzymatic, and accessory genes ...
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Distinguishing features of an infectious molecular clone of the highly divergent and noncytopathic human immunodeficiency virus type 2 UC1 strain.
... Phylogenetic analysis of the UClmc Gag and Env proteins demonstrated that this is the first completely sequenced full-length infectious molecular clone of an HIV-2 strain in the second H[r] ...
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Thymic Lesions in Cats Infected with a Pathogenic Molecular Clone or an ORF-A/2-Deficient Molecular Clone of Feline Immunodeficiency Virus
... ORF-A/2-deficient molecular clone. The infectious FIV mo- lecular clone JSY3 was obtained from Wayne ...JSY3⌬ORF-A/2 molecular clone was con- structed from JSY3 by site-specific ...The ...
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Development and Characterization of an In Vivo Pathogenic Molecular Clone of Equine Infectious Anemia Virus
... of molecular clone 19- 2-6A from nucleotide position 1 in the 59 LTR to the NcoI site at position 4889 (data not ...during molecular cloning ...
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Equine infectious anemia virus derived from a molecular clone persistently infects horses.
... TRONICK2 Department of Microbiology, Pathology & Parasitology, North Carolina State University, Box 8401, College of Veterinary Medicine, Raleigh, North Carolina 276061; Laboratory of Ce[r] ...
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Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice.
... The recombinant clones of minute virus of mice were infectious when transfected into monolayers of human 324K cells and produced virus plaques with an efficiency of about 6% that obtaine[r] ...
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Identification of novel monocistronic HTLV 1 mRNAs encoding functional Rex isoforms
... HTLV-1 molecular clone ACH (Figure 3b), and in PBMCs from HTLV-1-infected ATLL Figure 2 Coding potential of the novel mRNA species encoding Rex-protein ...
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Adaptation of Chimeric Retroviruses In Vitro and In Vivo: Isolation of Avian Retroviral Vectors with Extended Host Range
... To better understand the nature of the mutation in the env gene of the adapted retroviral vector RCASBP-M2C4070A, we transfected the DF-1 cells with the infectious molecular clone of the[r] ...
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Mutations in the tat Gene Are Responsible for Human Immunodeficiency Virus Type 1 Postintegration Latency in the U1 Cell Line
... context of virus infection, this mutation was introduced into the HIV molecular clone, pILIC (16) by site-directed mutagen- esis. A PvuII-PvuII fragment (nt 4501 to 7231; 11 5 mRNA start site) containing ...
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Differential glycosylation, virion incorporation, and sensitivity to neutralizing antibodies of human immunodeficiency virus type 1 envelope produced from infected primary T-lymphocyte and macrophage cultures.
... The finding that gp120 produced in PBMCs and MDMs is differentially glycosylated was intriguing since it has been pre- viously reported that (i) envelope glycosylation can influence the ability of antibodies to ...
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Simian immunodeficiency viruses from African green monkeys display unusual genetic diversity.
... To investigate this hypothesis, we determined the entire nucleotide sequence of an infectious proviral molecular clone of SIVagm 155-4 and partial sequences long terminal repeat and Gag [r] ...
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The caprine arthritis encephalitis virus tat gene is dispensable for efficient viral replication in vitro and in vivo.
... infectious molecular clone of CAEV were used to study their replication after transfection or infection of primary goat synovial membrane cells and of blood-derived mononuclear cells or ...
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Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection.
... To characterize nef functionally, we created chimeric viruses that contain nef derived from long-term survivors. Commonly, this process is accomplished by enzyme digestion and ligation (21). However, this technique is ...
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Disease Induction by Virus Derived from Molecular Clones of Equine Infectious Anemia Virus
... Sequence analysis. The env region sequences of clones p19/ wenv16 and p19/wenv17 were determined and were compared to the sequence of the env region of the avirulent molecular clone, pSPeiav19. As the ...
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Molecular cloning and characterization of the RNA packaging-defective retrovirus SE21Q1b.
... To determine the specificity of RNA packaging of virus derived from the SE21Q1b molecular clone and the SE2lQlb/ASLV hybrid provirus constructs, viral particles were harvested from SE21Q[r] ...
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Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.
... We have used these clones to further define regions of nucleic acid homology between CAEV and a molecular clone of visna virus 25 by heteroduplex mapping and molecular hybridization anal[r] ...
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Molecular Epidemiology over an 11 Year Period (2000 to 2010) of Extended Spectrum β Lactamase Producing Escherichia coli Causing Bacteremia in a Centralized Canadian Region
... A study was designed to assess the importance of sequence types among extended-spectrum  -lactamase (ESBL)-producing Escherichia coli isolates causing bacteremia over an 11-year period (2000 to 2010) in a centralized ...
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