Molecular hybridization
Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization
... Hepatitis A virus HAV RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA.. Hybridization was approximately 10-[r] ...
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SYNTHESIS AND PRELIMINARY ANTICANCER STUDY OF 5-FLUOROURACIL-PHTHALIMIDE CONJUGATE UTILIZING MOLECULAR HYBRIDIZATION APPROACH
... world. Molecular hybridization is very interesting approach in design of drug and development depends on the combination of pharmacophoric moieties of two pharmacologically active compounds results in a new ...
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Divergence of baboon endogenous type C virogenes in primates: genomic viral RNA in molecular hybridization experiments.
... Divergence of Baboon Endogenous Type C Virogenes in Primates: Genomic Viral RNA in Molecular Hybridization Experiments LAWRENCE DONEHOWER, FLOSSIE WONG-STAAL, AND DAVID GILLESPIE* Labora[r] ...
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Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes
... Enteroviruses were specifically detected in crude clinical specimens or in cell cultures in which the viruses amplified by dot hybridization by using poliovirus type 1-derived, subgenomi[r] ...
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Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization.
... DISCUSSION Filter hybridization in RNA excess before liquid hybridization in DNA excess was used to generate a viral RNA probe enriched for sequences complementary to reiterated viral DN[r] ...
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Detection of Baboon Type C Viral Sequences in Various Primate Tissues by Molecular Hybridization
... No nucleic acid sequences homologous to [3H ]DNA transcripts prepared from type C viruses isolated from tumor tissue of a woolly monkey and a gibbon ape could be detected in any primate [r] ...
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Conserved Region of Mammalian Retrovirus RNA
... It is generally accepted that under stringent conditions of molecular hybridization, type C viruses of different mammalian species rarely Downloaded from http://jvi.asm.org/ on November [r] ...
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Screening for hepatitis B virus in healthy blood donors by molecular DNA hybridization analysis
... A DNA molecular hybridization technique employing a purified adw subtype hepatitis B virus HBV cloned DNA of 3.2 kilobase pairs as a probe was used to screen for the presence of HBV DNA [r] ...
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Molecular genetic analysis of cervical cancer using fluorescence in situ hybridization and comparative genomic hybridization
... The concept of applying molecular hybridization directly to cytological material was initially pioneered by Gall and Pardue, (1969). During the following decade, application was restricted to highly ...
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Analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology.
... In situ molecular hybridization for detection of Aleutian mink disease parvovirus DNA by using strand-specific probes: identification of target cells for viral replication in cell cultur[r] ...
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Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.
... We have used these clones to further define regions of nucleic acid homology between CAEV and a molecular clone of visna virus 25 by heteroduplex mapping and molecular hybridization anal[r] ...
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Prospective target assessment and multimodal prediction of survival for personalized and risk-adapted treatment strategies in multiple myeloma in the GMMG-MM5 multicenter trial
... prospective molecular analysis and reporting, as opposed to being conducted in a pro- spective clinical ...on molecular diagnostics such as GEP or ...
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Prophage Carriage as a Molecular Epidemiological Marker in Streptococcus pneumoniae
... Variation of the lytA hybridization pattern with clonal type. A number of clinical isolates representing a variety of sero- types and antibiotypes and six different PFGE types and 18 different PFGE subtypes were ...
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Physiological effects of KDM5C on neural crest migration and eye formation during vertebrate development
... We further investigated the roles of KDM5C at the molecular level during eye development by coinjecting kdm5c MO and β-galactosidase mRNA unilaterally into one dorsal blastomere of eight-cell stage embryos. WISH ...
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Submicroscopic copy-number variations associated with 46,XY disorders of sex development
... NR0B1 is a transcription factor gene isolated from the dosage-sensitive sex reversal adrenal hypoplasia congenita critical region on Xp21 [24]. Overdosage of NR0B1 is a well-known cause of syndromic 46,XY DSD in ...
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PAX5 fusion genes in t(7;9)(q11.2;p13) leukemia: a case report and review of the literature
... Results: Using conventional cytogenetics, fluorescence in situ hybridization (FISH), and molecular methods, an additional case with a der(9)t(7;9)(q11.23;p13) resulting in the expression of a PAX5-ELN ...
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Evolutionarily new sequences expressed in tumors
... Southern hybridization analysis reveals only homology sequences in chicken genome for AA166653- specific ...Southern hybridization that a sequence homologous to the human AA166653-specific ...
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Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.
... The gene coding for this processed protein and its lower-molecular-weight precursor was mapped within the small unique region of the genome by hybridization of mRNA to cloned fragments o[r] ...
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Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization
... through E3, were derived from conserved sequences in the 5'-nontranslated region of the enterovirus genome, while the CVB3 genome-specific primers, B3-1 and B3-2, were derived from the V[r] ...
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Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding
... complex of agronomic parameters e.g. grain quality, resistance to biotic and abiotic stresses. Progress in the field of biochemistry and molecular biology has enabled to apply another type of biochemical- genetic ...
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