monocyte-derived dendritic cells
Evaluation of Feline Monocyte-Derived Dendritic Cells Loaded with Internally Inactivated Virus as a Vaccine against Feline Immunodeficiency Virus
... Dendritic cells are the only antigen-presenting cells that can present exogenous antigens to both helper and cytolytic T cells and prime Th1-type or Th2-type cellular immune ...functions, ...
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DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
... dendritiform cells of an increased accessory potency showing reduced Mel ...into monocyte derived dendritic cells ...the cells will acquire most of DC criteria; functionally, ...
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Varying Effects of Different β-Glucans on the Maturation of Porcine Monocyte-Derived Dendritic Cells
... Since dendritic cells (DCs) are the first cells that are encountered after an antigen is taken up by the intestinal epithelial cell barrier, we decided to investigate the effect of two concentrations ...
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Nsp1α of Porcine Reproductive and Respiratory Syndrome Virus Strain BB0907 Impairs the Function of Monocyte-Derived Dendritic Cells via the Release of Soluble CD83
... of monocyte/macrophage cell development, a reduction in antiviral and inflam- matory cytokines, and an increased secretion of immunosuppressive cytokines ...in monocyte- derived dendritic ...
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SAMHD1 Limits HIV-1 Antigen Presentation by Monocyte-Derived Dendritic Cells
... Monocyte-derived dendritic cells (MDDC) stimulate CD8 ⴙ cytotoxic T lymphocytes (CTL) by presenting endogenous and exog- enous viral peptides via major histocompatibility complex class I ...
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Immunotherapy of Malignant Melanoma with Tumor Lysate Pulsed Autologous Monocyte Derived Dendritic Cells
... tumor cells were dispersed to create a single-cell suspension. The cells were lysed by four to five freeze cycles (on liquid nitrogen) and thaw cycles (room ...
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Generation of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison
... Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in ...autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC ...
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Quantitative Expression and Virus Transmission Analysis of DC-SIGN on Monocyte-Derived Dendritic Cells
... temperature. Cells were collected onto Brandel grade GF/B filters with wash buffer (same as binding buffer plus 150 mM sodium chloride and no BSA) using a cell ...DC-SIGN/DC-SIGNR-expressing cells were ...
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Foal Monocyte-Derived Dendritic Cells Become Activated upon Rhodococcus equi Infection
... Real-time reverse transcription (RT)-PCRs for cytokine mRNA expression. Isolation of total RNA from infected and noninfected mMOs and mDCs was performed with an RNeasy Mini Kit (Qiagen). The RNA product was treated with ...
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Alteration of T cell immunity by lentiviral transduction of human monocyte derived dendritic cells
... was derived and maintained in GM-CSF and IL-4 supplemented ...T cells for 10 days, the T cells were reactivated and analyzed for Th functions by ICCS to determine intracellular expression of IFN-γ ...
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HIV 2 infects resting CD4+ T cells but not monocyte derived dendritic cells
... diving cells, such as monocytes, macrophages, dendritic cells and non-activated CD4+ lymphocytes ...dividing cells such as activated CD4+ T cells, SAMHD1 is phosphorylated and does not ...
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Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods
... 0.01% of all blood cells). To overcome this problem, high amounts of human DCs are generated in vitro from pre- cursors, CD14 pos (CD14 + ) monocytes or CD34 + cells, isolated from peripheral blood [7]. ...
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Severe Acute Respiratory Syndrome Coronavirus Fails To Activate Cytokine-Mediated Innate Immune Responses in Cultured Human Monocyte-Derived Dendritic Cells
... A549 cells, mono- cyte-derived macrophages, and DCs, since SCV-specific sub- genomic mRNAs were ...expression. Monocyte-derived human DCs were infected with SCV (MOI, 5) followed by fixing of ...
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Infection of human monocyte derived dendritic cells by ANDES Hantavirus enhances pro inflammatory state, the secretion of active MMP 9 and indirectly enhances endothelial permeability
... antigen-presenting cells in the antiviral immune ...antigen-presenting cells (APC), a process essential for the initiation and modula- tion of T cell-mediated immune ...presenting cells (APCs) such ...
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Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis
... human dendritic cells and ...human monocyte derived DC phagocytose ...human monocyte-derived dendritic cells in vitro and infected DCs show some up regulation of ...
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S100A9 protein is a novel ligand for the CD85j receptor and its interaction is implicated in the control of HIV 1 replication by NK cells
... to dendritic cells, NK cells and T lym- phocytes ...tumor cells, has been suggested to be an escape mechanism of gamma delta T-cell antitumoral activity [13] or continuous ligation of CD85j on ...
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Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus
... To further understand the differential activation of DCs by RSV and HMPV, we investigated herein whether these viruses induce distinct profiles of microRNAs (miRNAs), a kind of small non-coding RNA molecules of 19-24 ...
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Complementary Antiviral Efficacy of Hydroxyurea and Protease Inhibitors in Human Immunodeficiency Virus-Infected Dendritic Cells and Lymphocytes
... Dendritic cells are susceptible to human immunodeficiency virus (HIV) infection and may transmit the virus to T cells in ...in dendritic cells because preclinical testing of ...
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HIV-1 Envelope Glycan Moieties Modulate HIV-1 Transmission
... to monocyte-derived macro- phages (MDMs), (iii) bound monocyte-derived dendritic cells (MoDCs), and (iv) trans-infected primary lymphocytes via ...TZMbl cells, MDMs, ...
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Fibroblast and T cells conditioned media induce maturation dendritic cell and promote T helper immune response
... T cells as represent- atives of type 1 and type 2 cytokine patterns, ...by monocyte derived DCs and respective primed T cells in the supernatant of mature DCs and MLR ...T cells ...
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