Mosquito cells
The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex
... the mosquito cell lines (C6/36 and Aag2) and the vertebrate cell line BHK-21, used for comparisons, was measured using the reduction of tetrazolium salts to examine proliferation in cells treated with ...
25
Synthesis of Genomic and Subgenomic RNA in Mosquito Cells Infected with Two Sindbis Virus nsP4 Mutants: Influence of Intracellular Nucleoside Triphosphate Concentrations
... in mosquito cells that had also been treated with ...SVstd-infected cells, SG RNA was made in excess of G RNA ...SVpzf-infected cells, more G RNA was made than SG RNA (lane ...SVstd-infected ...
9
Comparative analysis of DNA vectors at mediating RNAi in Anopheles mosquito cells and larvae
... Fig.·3. Quantification of RNA interference in A. gambiae Sua 4.0 cells and A. stephensi larvae. (A) A. gambiae Sua 4.0 cells co- transfected with pMinEGFP and one of the dsRNA-expressing plasmids. (B) ...
7
The Host Range Phenotype Displayed by a Sindbis Virus Glycoprotein Variant Results from Virion Aggregation and Retention on the Surface of Mosquito Cells
... differences were noted between these viruses in the cell agita- tion assay. Specifically, agitation of infected mosquito cells had a much greater effect on the extracellular titer of NE2G216 than on that of ...
9
S-Phase-Dependent Enhancement of Dengue Virus 2 Replication in Mosquito Cells, but Not in Human Cells
... stall cells in S-phase do not inhibit other cellular metabolic processes, such as translation and membrane biogenesis (38, ...prokaryotic cells (37, ...malian cells (30, 38), although whether ...
13
Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells
... their replication, which may induce RNAi in the host cells. A new explanation for mosquitoes’ non-pathogenic and persistent infections of DEN is that RNAi could be an important modulator [19]. Exogenous long ...
8
Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
... the cells for viral titer estimations, and fresh medium supplemented with the inhibitor was added back to the ...the cells were monitored visually throughout infection in the absence of the inhibitor; ...
10
La Crosse Bunyavirus Nonstructural Protein NSs Serves To Suppress the Type I Interferon System of Mammalian Hosts
... a mosquito-transmitted member of the Bunyaviridae family that causes severe encephalitis in ...mammalian cells had suggested two different functions, namely induction of apoptosis and inhibition of RNA ...
9
Mosquito Rasputin interacts with chikungunya virus nsP3 and determines the infection rate in Aedes albopictus
... and mosquito cells the cellular protein Ras-GAP SH3 domain binding proteins (G3BPs) were found in nsP3-granules [28, ...and cells expressing nsP3 were unable to ...
15
Differential RNA Sequence Requirement for Dengue Virus Replication in Mosquito and Mammalian Cells
... in mosquito cells, we de- signed mutants carrying single substitutions in each position of the loop and evaluated viral replication in both cell ...into mosquito cells showed that mutants Mut ...
8
Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus.
... infected cells of each ...that mosquito cells persistently infected with Sindbis virus are broadly able to exclude other alphaviruses but that exclusion is restricted to members of the alphavirus ...
5
Biological and Phylogenetic Characteristics of Yellow Fever Virus Lineages from West Africa
... liver cells, it is surprising that the growth of strain 333 more closely resembles that of the reference strains isolated from YF patients than that of the other mosquito ...male mosquito or a female ...
13
Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells
... in mosquito and mammalian ...in mosquito cells form around acidic vesicles that contain the viral ...mammalian cells, the double-stranded RNA (dsRNA)-containing RCs do not associate with ...
16
A Bunyamwera Virus Minireplicon System in Mosquito Cells
... in mosquito cells expressing T7 RNA polymerase. C6-IBT7/3 cells were transfected with various combinations of pT7AcL, pT7AcN, and pT7riboBUNMREN (⫺) along with pT7AcLuc as an internal transfection ...
7
Does Japanese encephalitis virus share the same cellular receptor with other mosquito borne flaviviruses on the C6/36 mosquito cells?
... C6/36 cells as a candi- date DV 2, 3, and 4 receptor ...midgut cells of Aedes aegypti and C6/36 cells have been recently identi- fied as receptors for the four serotypes of DV ...C6/36 cells, ...
7
Role of Bunyamwera orthobunyavirus NSs protein in infection of mosquito cells
... in mosquito cells To analyse how BUNV spreads in mosquito cells, ...U4.4 cells were infected with recombinant BUNV expressing green fluorescent protein [41], either rBUNGc- eGFP or ...
12
Comparative Characterization of the Sindbis Virus Proteome from Mammalian and Invertebrate Hosts Identifies nsP2 as a Component of the Virion and Sorting Nexin 5 as a Significant Host Factor for Alphavirus Replication
... It is of special interest that junctional adhesion molecule C (JAMC), claudin (CLDN), and myosin are all components of the epithelial cell tight junctions (TJ) that provide a physical barrier between cells (59). ...
17
The Asian bush mosquito Aedes japonicus japonicus (Diptera: Culicidae) in Europe, 17 years after its first detection, with a focus on monitoring methods
... tiger mosquito Aedes (Stegomyia) albopictus (Skuse, 1895) and the yel- low fever mosquito Aedes (Stegomyia) aegypti (Linnaeus, 1762) were considered responsible for several disease outbreaks in Europe after ...
13
Ultrastructural Characterization and Three-Dimensional Architecture of Replication Sites in Dengue Virus-Infected Mosquito Cells
... C6/36 cells. At 48 h p.i., infected cells were fixed and reacted with antibodies specific for the following viral proteins: C, prM, E, NS1, NS3, and ...infected cells compared to mock-infected ...
11
Host-dependent evolution of the Sindbis virus promoter for subgenomic mRNA synthesis.
... BHK-21 cells at 30 8 C. Growth and RNA analysis in C7-10 cells. Cells were infected at an MOI of 5 (titers determined with C7-10 cells) for growth and RNA ...
7