mouse embryonic fibroblast cells
Lack of p53 function promotes radiation-induced mitotic catastrophe in mouse embryonic fibroblast cells
... 12(1) cells (Figure 5) shows that following 10 Gy irradiation, similar transient delays late in the cell cycle are present as it is in the mutant p53 MEF 10(1) cells, with the exception that the increase in ...
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Biocompatibility effects of biologically synthesized graphene in primary mouse embryonic fibroblast cells
... a mouse fibroblast cell line (L-929) to assess the cytotoxicity; the results suggested that the cells adhered and proliferated on graphene film well than carbon nanotubes, which indi- cated that the ...
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In vitro apoptotic and DNA damaging potential of nanobarium oxide
... Even though there are existing studies on the toxicity of barium nanoparticles, the underlying machinery inducing toxicity remains unexplored till now. Moreover none of the studies, so far, have explored the adverse ...
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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells
... stem cells (ESC) were first derived from the inner cell mass of the murine blastocyst in 1981 ...origin. Mouse embryonic fibroblast (MEF) feeder cells were traditionally used to support ...
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Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells
... stem cells, Pringle and colleagues mixed Matrigel and collagen to drive differentiation of cultured spheres into branching and lobular structures ...stem/progenitor cells and drive its acinar-like ...
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Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells
... of mouse ESCs at a ground state of self-renewal in the absence of LIF and serum was recently reported using two inhibitors (2i) of fibroblast growth factor/extracellular signal-related kinase 1/2 and ...
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FRS2α is Essential for the Fibroblast Growth Factor to Regulate the mTOR Pathway and Autophagy in Mouse Embryonic Fibroblasts
... The results suggest that the autopha- gy-suppressing signals of the FGF signaling axis are different from the FiRE-activating signals that are mediated by the four Grb2-binding sites additively, since substitution of ...
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The effects of 17 alpha-estradiol to inhibit inflammation in vitro
... Briefly, mouse embryonic fibroblast (MEF) cells were generated from C57BL/6J WT and total body ERα knock- out (ERKO) ...°C. Cells were passaged once they reached ~ 90% ...
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Reprogramming of Mouse Embryonic Fibroblasts to Mouse Hematopoietic Progenitor Cells
... stem cells (HSCs) show great potential for clinical applications, although obtaining matched HSCs for specific applications and efficiently expanding HSCs in vitro are ...somatic cells into hematopoietic ...
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Differentiation roadmap of embryonic Sertoli cells derived from mouse embryonic stem cells
... mES cells to eSCs [6, 8, 9, 23–25, 34, 39, ...mES cells by replacing the mouse embryo fibroblast (MEF) feeder with TM4 feeder cells, providing a big num- ber of quality mES ...mES ...
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LMP1 of Epstein-Barr Virus Induces Proliferation of Primary Mouse Embryonic Fibroblasts and Cooperatively Transforms the Cells with a p16-Insensitive CDK4 Oncogene
... primary cells, the inactivation of Rb, and the suppression of p53 activities through direct ...rodent fibroblast cell lines, do not effectively immortalize primary cells without the additional ...
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Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
... of cells i.e., mouse embryonic fibroblast (NIH3T3), primary cul- tured normal human dermal fibroblast (nHDF), and hu- man fibrosarcoma cells ...in cells to assess the ...
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The effect of mouse embryonic fibroblast in direct differentiation of mouse embryonic stem cells
... ES cells showed that several types of cells can be enriched in culture either by the addition of growth factors or by introduction of transcription factors ...of mouse ES cells into different ...
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Transient accumulation of 5 carboxylcytosine indicates involvement of active demethylation in lineage specification of neural stem cells
... and mouse embryonic sections using human adult-derived fibroblasts and adult mouse brain tissue as controls (Figures ...the fibroblast cultures (Figure 1A). Likewise, although some ...
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Isolation and differentiation of mouse embryonic stem cells
... ES cells early in expansion stage and later in colony formation ...the cells to save time and money. It was previously reported that mouse ES cells in contrast to human ES cells only ...
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Visualizing centrosome movement in mouse embryonic stem cells
... Single cells of the selected population were put into a single well of a 96-well ...single cells could grow into bigger ...Therefore, cells were trypsinated and placed in ESC medium with 10% DMSO and ...
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Estrogenic in vitro assay on mouse embryonic Leydig cells
... respectively. Cells were incubated over night at 4 ° C with anti-StAR (1:250) (Santa Cruz, USA, ...BSA. Cells were washed and transferred in secondary antibodies, anti-rabbit (StAR), anti-goat (MIS) and ...
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Cytomegalovirus InducesInterferon-Stimulated Gene Expression and Is Attenuated by Interferon in the Developing Brain
... of mouse neuronal cultures, human astrocytes, and human primary brain cultures with the double-stranded RNA poly(I:C) protects cells from mCMV or hCMV ...in cells, including microglia through ...
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Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex
... Considering that Ddx56 was found in Oct4-related pro- tein in mESCs by mass spectrometry, we then tried to confirm the interaction between Ddx56 and Oct4. We overexpressed Flag-Ddx56 in mESCs and performed im- ...
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Alginate-based Hydrogels for Central Nervous System Tissue Regeneration
... control embryonic stem cell identity, they found that reprogramming of mouse and human embryonic fibroblasts (MEF) into induced pluripotent stem cells (iPSCs) can be achieved by the forced ...
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