Mouse embryonic stem cells (mESCs)
Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells
... Mouse embryonic stem cells (mESCs) derived from the inner cell mass of blastocysts are considered multi- pluripotent with the characteristic of rapid self-renewal ...study embryonic ...
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Mapping molecular pathways for embryonic Sertoli cells derivation based on differentiation model of mouse embryonic stem cells
... Sertoli cells are as follows: the cells have a great variety in genital ridges and coelomic epithelium, the relevant factors have complicated interaction, the target genes are hard to manipulate in vivo ...
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Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex
... E14 cells were transfected with control oligos (NC) or three different oligos targeting Ddx56 (si-1, si- ...h, cells were collected, and knockdown efficiency was de- tected by RT – ...E14 cells. (B) ...
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TET dependent regulation of retrotransposable elements in mouse embryonic stem cells
... Background: Ten-eleven translocation (TET) enzymes oxidise DNA methylation as part of an active demethylation pathway. Despite extensive research into the role of TETs in genome regulation, little is known about their ...
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Testing of a culture protocol for converting mouse embryonic stem cells into neurons
... the cells obtained by this culture protocol resembled ...0 cells, that had just been thawed and plated, were stained with this marker, they did not ...the cells towards neuronal ...neuron-like ...
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Visualizing centrosome movement in mouse embryonic stem cells
... the cells over time in 3D structures, which we aim to do in the future with our gastruloids ...expressing cells, the best clone needs to be selected before experiments can ...
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Temporal specification of blood progenitors from mouse embryonic stem cells and induced pluripotent stem cells
... the cells dissociated and the appropriate populations isolated by cell ...sorted cells were either cultured at 250,000 cells/ml in 24-well ULA dishes (Costar) or at 30,000 cells/100 ml in ...
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Generation of thalamic neurons from mouse embryonic stem cells
... 20,000 cells per neonate). The grafted cells were observed in subcortical regions such as caudate putamen, white matter and cortical ...these cells were not labeled with an oligodendrocyte marker, ...
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Investigating the nephrogenic potential of mouse embryonic stem cells and their derivatives
... Embryonic stem cells (ESC) are derived from the inner cell mass (ICM) of blastocyst at stage 4 to 5 days of the developing ...First, mouse ES cells were isolated in 1981 independently ...
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Rabbit antiserum to mouse embryonic stem cells delays compaction of mouse preimplantation embryos
... feeder cells were removed by feeder-free culture and harvested by ...8 cells in PBS were emulsified in Freund's complete adjuvant (FCA) [1 volume antigen: 2 volumes FCA] and injected into multiple ...
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Germline potential of parthenogenetic haploid mouse embryonic stem cells
... epiblast stem cells (EpiSCs) (Brons et ...diploid cells was already apparent and after one passage the fraction of haploid cells had been lost ...ESCs. Mouse ESCs are normally excluded ...
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Specialized mouse embryonic stem cells for studying vascular development
... female mouse was sacrificed by cervical ...on mouse embryonic fibroblasts (MEF). The cells were initially fed ESC Culture Medium consisting of Knockout Dulbecco’s Modified Eagle’s Medium ...
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Moesin Is Not a Receptor for Measles Virus Entry into Mouse Embryonic Stem Cells
... CHO cells or the murine or human cell lines tested by flow cytometry with MAbs M22 and CR22 (data not ...moesin-positive cells and examined the syncytia ...positive cells (data not shown), consistent ...
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Genome wide gene expression analysis in mouse embryonic stem cells
... the cells exhibited the properties of stem cells ...that cells were capable of long-term ...whether cells could be subcultured after freez- ing, thawing and ...MES3 cells were ...
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Functional properties of motoneurons derived from mouse embryonic stem cells
... ES cells is a critical step to- ward the development of cell replacement therapies for neurodegenerative diseases in which discrete populations of neurons are ...ES cells exposed to RA and Hh-Ag express ...
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Genome-wide barcoded transposon screen for cancer drug sensitivity in haploid mouse embryonic stem cells.
... pH 8. Primary PCR was carried out using Q5 polymerase (NEB) in a 50 μl reaction volume using 20 μl ligated DNA. Primer sequences and cycling conditions can be found in Supplementary Data. Samples were paired-end ...
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Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells
... Cell lysate of mouse ESCs in Co-IP buffer containing 10 mM Hepes (pH 7.6), 250 mM sodium chloride, 0.1% Nonidet P-40, 5 mM EDTA, 1 mM phenylmetha- nesulfonyl fluoride and 1 mM sodium fluoride was col- lected and ...
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Isolation and differentiation of mouse embryonic stem cells
... the cells in 96-well dishs up to 3 more passages every 3 days until the ES colony morphology appear in the expansion ...the cells became confluent enough for passage in 4-well dish ...the cells must ...
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The effect of mouse embryonic fibroblast in direct differentiation of mouse embryonic stem cells
... ES cells are capable of differentiating into many cell ...these cells for medical applications, including cellular transplantation, has attracted more attention in the field of ES cells isolation and ...
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Differentiation roadmap of embryonic Sertoli cells derived from mouse embryonic stem cells
... differentiated cells spreading around were left, a clear growth trend of FasL − /AMH + cells and FasL + /AMH + cells was observed ...+ cells rapidly declined when eSCs had an obvious increase ...
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