mouse PrP
Species-Independent Inhibition of Abnormal Prion Protein (PrP) Formation by a Peptide Containing a Conserved PrP Sequence
... experiments, PrP conversion was inhibited by a peptide composed of hamster PrP residues 109 to 141, suggesting that this region of the PrP molecule plays a crucial role in the conversion ...used ...
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Glycosylphosphatidylinositol Anchor-Dependent Stimulation Pathway Required for Generation of Baculovirus-Derived Recombinant Scrapie Prion Protein
... of mouse PrP in a baculovirus-insect cell ...of mouse PrP was amplified by PCR from mouse brain cDNA using the forward primer 5⬘-CGGGATCCGCCACC ATGGCGAACCTTGGCTAC-3 ⬘ and reverse primer ...
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Multiple Amino Acid Residues within the Rabbit Prion Protein Inhibit Formation of Its Abnormal Isoform
... L42-positive PrP-res and did not affect the accumulation of total PrP-res ...to PrP- res and interfered with the accumulation of total PrP-res ...total PrP-res accumulation ...of ...
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Cells Expressing Anchorless Prion Protein Are Resistant to Scrapie Infection
... anchorless mouse PrP con- taining the epitope to mouse monoclonal antibody 3F4 (Mo3F4 GPI ⫹ PrP-sen and Mo3F4 GPI ⫺ PrP-sen, respectively) have been previously described ...20). ...
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Preparation and characterization of antibodies against mouse prion protein (PrP) peptides.
... of mouse prion protein (PrP), which were synthesized by using a multiple antigenic peptide (MAP) ...to PrP were examined by an enzyme-linked immunosorbent assay (ELISA), Western immunoblotting (WB), ...
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Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors
... recombinant mouse PrP fibrils, both recombinant bank vole PrP molecules were able to form amyloid fibrils with virtually no lag phases, through with different kinetics ...vole PrP proteins ...
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Identification of Two Prion Protein Regions That Modify Scrapie Incubation Time
... full-length mouse PrP and MHM2(⌬23–88), (ii) decreased prion conversion effi- ciency caused by removal of the N terminus, (iii) insufficient expression of the MHM2(⌬23–88) transgene, and (iv) de- creased ...
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Cell Biological Studies of the Prion Protein
... of PrP, we have expressed in transfected CHO cells mouse PrP molecules in which N- glycosylation has been blocked either by treatment with the drug tunicamycin, or by substitution of alanine for ...
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A single hamster PrP amino acid blocks conversion to protease-resistant PrP in scrapie-infected mouse neuroblastoma cells.
... a mouse PrP gene from which the NaeI-BstEII fragment had been ...hamster PrP except for two mouse PrP amino acids in the NaeI-BstEII fragment and are shown in Table ...
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PrP Knockout Cells Expressing Transmembrane PrP Resist Prion Infection
... WT mouse PrP open reading frame (a kind gift from Ryuichiro Atarashi, Nagasaki University, Nagasaki, Japan) flanked by HindIII and XbaI sites on the 5= and 3= ends, respectively, and cloned into a ...WT ...
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Biological Effects and Use of PrPSc- and PrP-Specific Antibodies Generated by Immunization with Purified Full-Length Native Mouse Prions
... SPR analysis. Binding kinetics was determined on a Biacore 1000 instrument (Biacore AB, Uppsala, Sweden). Recombinant mouse PrP (1 M) was diluted in 10 mM sodium acetate (NaOAc) (pH 4.5) and was ...
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Deletion of β-Strand and α-Helix Secondary Structure in Normal Prion Protein Inhibits Formation of Its Protease-Resistant Isoform
... that PrP- res formation occurs either on the cell surface and/or along the endocytic pathway (3, 8, 10, 34, ...inhibit PrP-res ...heterologous PrP-sen. The higher the ratio of het- erologous to ...
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Biochemical Fingerprints of Prion Infection: Accumulations of Aberrant Full-Length and N-Terminally Truncated PrP Species Are Common Features in Mouse Prion Disease
... -infected mice have additional N-terminally truncated PrP species. We also immunoblotted each brain homogenate with MAb 8H4, which recognizes all described PrP species. When immunoblotted with MAb 8H4, all ...
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Clinical Experience With Haemophilus influenzae Type b Conjugate Vaccines
... Three conjugate vaccines have been tested for immunogenicity in infants in Finland: PRP-D, a conjugate of PRP with diphtheria toxoi&2; HbOC, a conjugate of PRP-derived oligosaccharid[r] ...
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Iodine contrast agents do not influence Platelet-Rich Plasma function at an early time point in vitro
... intra-articular PRP infiltration of the pathological femoral-patellar ...of PRP were sampled before intra-articular injection. First, PRP samples were mixed with 50 μ l of 2 widely used ICA: ...
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Ex vivomammalian prions are formed of paired double helical prion protein fibrils
... vivo PrP rods are physically associated with prion ...digestion. PrP rods (shown here immunogold-labelled with an anti-PrP monoclonal antibody) are the only visible protein ...of PrP rods (RML ...
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Haemophilus influenzae Type b Conjugate Vaccine (Meningicoccal Protein Conjugate): Immunogenicity and Safety at Various Doses
... effect of dose of PRP-OMPC on anti-PRP antibody levels in children and infants as young as 2 months of age.. In addition, the safety of PRP-OMPC at different doses was.[r] ...
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Mouse-Adapted Scrapie Infection of SN56 Cells: Greater Efficiency with Microsome-Associated versus Purified PrP-res
... purified PrP-res does not alter levels of long-term PrP-res ...purified PrP-res preparations were supple- mented with brain microsomes prepared from normal mice in amounts normalized for total ...
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Mouse Prion Protein (PrP) Segment 100 to 104 Regulates Conversion of PrPC to PrPSc in Prion-Infected Neuroblastoma Cells
... chicken PrP was obtained by PCR using primer set 2 and genomic DNA from a broiler chicken as the ...chicken PrP ORF into a ...chicken PrP tagged by the 3F4 epitope (designated C4) ...chicken ...
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Positive effect on spinal fusion by the combination of platelet-rich plasma and collagen-mineral scaffold using lumbar posterolateral fusion model in rats
... inside PRP [15]. Many studies examine the efficacy of PRP on bone fusion; however, the results were not consistent [16, ...Concentrate PRP fibrin gel is suitable for use inside the bone cavity but ...
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