MRC-5
Enhanced detection of cytomegalovirus in MRC 5 shell vials
... RESULTS When the HCMV AD169 stock virus was grown in treated and untreated MRC-5 cells from three different commercial suppliers, the cells from Whittaker Bioproducts proved to be consid[r] ...
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Practical protocol for cytomegalovirus isolation: use of MRC 5 cell monolayers incubated for 2 weeks
... In the second segment, one tube of WI-38 cells was compared with one tube of MRC-5 cells over a 14-day period for susceptibility to CMV infection and speed of development of viral cytopa[r] ...
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MRC 5 fibroblast conditioned medium influences multiple pathways regulating invasion, migration, proliferation, and apoptosis in hepatocellular carcinoma
... of MRC-5-CM on Bel- 7402 and MHCC-LM3 cell viability, we evaluated the expression of cell cycle- and apoptosis-related ...results, MRC-5 induced G1 phase arrest in Bel-7402 cells and S phase ...
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Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells.
... Since even the most efficiently growing mutant MR3 did not fully reproduce the growth phenotype of the MRC-5/9 virus in cell culture, it seems likely that the MRC-5-specific mutations in[r] ...
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Original Article Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells
... Abstract: Human cytomegalovirus (HCMV) is major cause of birth defects upon congenital infection as well as mor- bidity in immunosuppressed populations and has evolved several mechanisms to evade IFN-I responses. ...
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Enhanced detection of cytomegalovirus in confluent MRC 5 cells treated with dexamethasone and dimethyl sulfoxide
... To determine whether dimethyl sulfoxide DMSO or dexamethasone DEX, which are known enhancers of HCMV, facilitates the detection of the virus in confluent cells, we tested both HCMV AD169[r] ...
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Varicella-Zoster Virus ORF12 Protein Activates the Phosphatidylinositol 3-Kinase/Akt Pathway To Regulate Cell Cycle Progression
... when MRC-5 cells were infected with ROka12DR (a rescued version of ROka12D in which a full-length copy of ORF12 is inserted into the ORF12 deletion mutant ROka12D [21]) and treated with the PI3K inhib- itor ...
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<p>Prostate Cancer Cellular Uptake of Ternary Titanate Nanotubes/CuFe<sub>2</sub>O<sub>4</sub>/Zn-Fe Mixed Metal Oxides Nanocomposite</p>
... Human prostate cancer PC-3 cell lines were obtained from the American-Type Culture Collection (ATCC, Rockville, MD). Mammalian cell lines: MRC-5 cells (Normal human Lung fi broblast cells) were acquired from ...
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Activities of ICP0 Involved in the Reversal of Silencing of Quiescent Herpes Simplex Virus 1
... genomes. MRC-5 cells were infected with d109 and incubated in reduced serum for 1 ...fluorescent MRC-5 cell is shown in each of the images of mock-, RF-, and RFBac-infected ...
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Seminal Plasma and Semen Amyloids Enhance Cytomegalovirus Infection in Cell Culture
... (v) 5 g of SEM/ml plus 5 g of ...and MRC-5 cells were carried out at an MOI of ...and MRC-5 (for HCMV) cells. As shown in Fig. 5, both MCMV (lower) and HCMV (upper) ...
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Vivek Vaidya, Snehal Agnihotri, Rajeev Dhere, Ravindra Muley, Sanjay Patil, Amit Patil, Arvind Panse
... of MRC-5 cells using both the recombinant trypsin solutions was comparable to conventional porcine ...on MRC-5 cells, sub-cultured till passage-60, using recombinant trypsin was comparable ...
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Middle East Respiratory Syndrome Coronavirus Infection Mediated by the Transmembrane Serine Protease TMPRSS2
... WI-38, MRC-5, and Calu-3 cells were infected with MERS-CoV in the presence of camostat (Camo; 10 M), EST (10 M), camostat plus EST (Camo ⫹ EST), or leupeptin (10 ...further 5 h at ...
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Parvovirus replication in normal and transformed human cells correlates with the nuclear translocation of the early protein NS1.
... We previously observed that H-1 infection of MRC-5 or W138 human diploid fibroblasts was nonproductive for infectious virus, but a net increase in virus could be obtained from the SV40-t[r] ...
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Direct inactivation of viruses by human granulocyte defensins.
... Finally, to test the possibility that HNP-1 may have some species-specific, interferonlike, cell-mediated antiviral activity, a human cell line, MRC-5, was treated with the peptide befor[r] ...
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Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking
... CHBE-Exo-treated MRC-5 cells were fixed with 1% formaldehyde for 10 ...follows: 5’-ACCTTATCTCT AATACACA-3’ (sense) and 5’-GGTGAAAAGAAA ATAATTACACA-3’ ...
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Sulforaphane attenuates pulmonary fibrosis by inhibiting the epithelial-mesenchymal transition
... of MRC-5 cells (a pulmonary fibroblast cell line) and sup- pressed the TGF-β1-induced overexpression of fibrogenic proteins (fibronectin, collagen I, and α-SMA) in these ...in MRC-5 ...in ...
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Glycosylation of herpes simplex virus type 1 gC in the presence of tunicamycin.
... The effect of tunicamycin on the synthesis of herpes simplex virus type 1 HSV-1 KOS glycoproteins was examined by analysis of the proteins synthesized in infected MRC-5 cell cultures mai[r] ...
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Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts
... An increase in the fibroblast cell population as well as an increase in collagen synthesis are key features of fibrotic dis- ease. Our observation that tryptase can stimulate both an in- crease in cell numbers and a ...
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Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen
... DISCUSSION The results of this study demonstrate that the use of a monoclonal antibody to a CMV early nuclear antigen in an IPA stain performed 48 h after inoculation of MRC-5 cell monol[r] ...
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Antiproliferative activity and induction of apoptotic by ethanolic extract of Alpinia galanga rhizhome in human breast carcinoma cell line
... are reporting for the first time that the ethanolic extract of galangal rhizome-induced cytotoxicity and apoptosis on MCF-7 cells versus MRC-5 cells. Much effort has been directed toward the effect of the ...
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