MRC-5 cells
Original Article Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells
... in MRC-5 cells was very high (Figure ...in MRC-5 cells transfected with miR-146a mimics or miR-146a ...in MRC-5cells (Figure ...
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Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells.
... Since even the most efficiently growing mutant MR3 did not fully reproduce the growth phenotype of the MRC-5/9 virus in cell culture, it seems likely that the MRC-5-specific mutations in[r] ...
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Enhanced detection of cytomegalovirus in confluent MRC 5 cells treated with dexamethasone and dimethyl sulfoxide
... To determine whether dimethyl sulfoxide DMSO or dexamethasone DEX, which are known enhancers of HCMV, facilitates the detection of the virus in confluent cells, we tested both HCMV AD169[r] ...
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Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking
... CHBE-Exo-treated MRC-5 cells were fixed with 1% formaldehyde for 10 ...follows: 5’-ACCTTATCTCT AATACACA-3’ (sense) and 5’-GGTGAAAAGAAA ATAATTACACA-3’ ...
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Varicella-Zoster Virus ORF49 Functions in the Efficient Production of Progeny Virus through Its Interaction with Essential Tegument Protein ORF44
... and MRC-5 cells; however, an effective trans-complementation system for ORF44 as for ORF49 was not successfully established, and at what step(s) in the lytic infection ORF44 essentially functions ...
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Varicella-Zoster Virus ORF12 Protein Activates the Phosphatidylinositol 3-Kinase/Akt Pathway To Regulate Cell Cycle Progression
... transfected cells can phosphorylate Akt ...VZV-infected cells. MRC-5 cells were infected with VZV ROka or ROka12D for 24 h and then were left untreated or were treated with the PI3K ...
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Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth
... in MRC-5 cells between 0 and 3 h, however, it induced a shift towards a lower free / bound NADH ratio over time in IMR-32 ...treated MRC-5 ...IMR-32 cells that have high ...
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Practical protocol for cytomegalovirus isolation: use of MRC 5 cell monolayers incubated for 2 weeks
... In the second segment, one tube of WI-38 cells was compared with one tube of MRC-5 cells over a 14-day period for susceptibility to CMV infection and speed of development of viral cytopa[r] ...
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Biologic and Immunologic Effects of Knockout of Human Cytomegalovirus pp65 Nuclear Localization Signal
... segregation; 5 genes involved in the cell cycle check point; and ...from MRC-5 cells transduced with rAAV pp65mIINLSKO, rAAV pp65mII, or rAAV pp65N was collected at various times ...
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<p>Prostate Cancer Cellular Uptake of Ternary Titanate Nanotubes/CuFe<sub>2</sub>O<sub>4</sub>/Zn-Fe Mixed Metal Oxides Nanocomposite</p>
... Human prostate cancer PC-3 cell lines were obtained from the American-Type Culture Collection (ATCC, Rockville, MD). Mammalian cell lines: MRC-5 cells (Normal human Lung fi broblast cells) were ...
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Neoplastic Transformation-Associated Stimulation of the In Vitro Resolution of Concatemer Junction Fragments from Minute Virus of Mice DNA
... these cells proved to be as active as extracts from MRC- 5V1 cells in sustaining the 39-to-39 junction resolution in the present assay (data not ...to cells express- ing the SV40 large T ...
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Prokaryotic expression, purification of a novel candidate tumor suppressor gene FUS1 and characterization of its polyclonal antibodies
... from MRC-5 cells was amplified by RT-PCR and cloned into prokaryotic expression vector ...for 5 h at ...and cells by western blot and immuno- histochemistry, Available purified rFUS1 ...
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Enhanced detection of cytomegalovirus in MRC 5 shell vials
... RESULTS When the HCMV AD169 stock virus was grown in treated and untreated MRC-5 cells from three different commercial suppliers, the cells from Whittaker Bioproducts proved to be consid[r] ...
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A three-nucleotide deletion in the UL97 open reading frame is responsible for the ganciclovir resistance of a human cytomegalovirus clinical isolate.
... 60-mm-diameter dish 16 to 20 h before transfection. Full-length infectious AD169 DNA (2 mg) and the UL97 fragments (0.2 mg) were cotransfected into MRC-5 cells showing a 75% degree of confluency by ...
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Activities of ICP0 Involved in the Reversal of Silencing of Quiescent Herpes Simplex Virus 1
... of MRC-5 cells with a virus that does not express immediate-early proteins, followed by superinfection with various viral mutants to quantify the ability of ICP0 variants to reactivate gene ...
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Vivek Vaidya, Snehal Agnihotri, Rajeev Dhere, Ravindra Muley, Sanjay Patil, Amit Patil, Arvind Panse
... of MRC-5 cells’ using 175 cm 2 tissue culture flaks was done using recombinant trypsin solutions from two different manufacturers as described earlier (Set-I & ...
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In vitro biological responses to nanofibrillated cellulose by human dermal, lung and immune cells: surface chemistry aspect
... Background: Nanocellulose, and particularly nanofibrillated cellulose (NFC), has been proposed for a diversity of applications in industry and in the biomedical field. Its unique physicochemical and structural features ...
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Genistein mediates the selective radiosensitizing effect in NSCLC A549 cells via inhibiting methylation of the keap1 gene promoter region
... A549 cells instead of MRC-5 cells (Figure ...A549 cells had low levels of GSH and GSH to GSSG ratio in both the basal and inducible groups; but in MRC-5 cells, due ...
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Sulforaphane attenuates pulmonary fibrosis by inhibiting the epithelial-mesenchymal transition
... epithelial cells, and proliferation and differentiation into myofibroblasts in fibroblasts [3, 4, 6, ...of MRC-5 cells (a pulmonary fibroblast cell line) and sup- pressed the TGF-β1-induced ...
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Inhibition of Dengue and Chikungunya Virus Infections by RIG-I-Mediated Type I Interferon-Independent Stimulation of the Innate Antiviral Response
... with 5=pppRNA inhibits CHIKV replication in a RIG-I-dependent manner. (A) MRC-5 cells were treated with ...or 5=pppRNA from 1 h prior to infection to 24 h postinfection with CHIKV ...
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