MSP-1
IMMUNOGENICITY OF THE MEROZOITE SURFACE PROTEIN-1 (MSP-1) OF HUMAN PLASMODIUM SP.
... using MSP-1 encounters numerous ...on MSP-1 are ...Plasmodium MSP-1 in non-human models , especially in rodent models, are unable to completely represent the safety, efficacy and ...
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Genetic diversity of Plasmodium falciparum isolates from Pahang, Malaysia based on MSP 1 and MSP 2 genes
... the MSP-1 positive cases harbored a single allele (monoclo- nal infection), and the polyclonal infection was reported in ...the MSP-2 posi- tive cases had monoclonal ...on MSP-1) of ...
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Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp 1 and msp 2 genes in Gublak, North West Ethiopia
... Data were entered and analysed using SPSS version 20 (SPSS Inc. Chicago, IL, USA). The msp-1 and msp-2 allelic frequencies were calculated. The mean multiplicity of infection (MOI) was calculated for ...
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Genetic diversity of the msp 1, msp 2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia
... Background: Determination of the genetic diversity of malaria parasites can inform the intensity of transmission and identify potential deficiencies in malaria control programmes. This study was conducted to characterize ...
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Temporal changes in genetic diversity of msp 1, msp 2, and msp 3 in Plasmodium falciparum isolates from Grande Comore Island after introduction of ACT
... (msp-1, msp- 2, and msp-3) have been widely used for characterization of parasite genetic diversity [9, ...of msp-1, msp- 2, and ...
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Solution structure of a Plasmodium falciparum AMA 1/MSP 1 chimeric protein vaccine candidate (PfCP 2 9) for malaria
... Malaria is one of the most serious life-threatening tropi- cal diseases in the world. Because of the rapid spread of drug-resistant parasites and insecticide-resistant mosqui- toes [1-4], new tools for control ...
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Genetic diversity of Plasmodium falciparum isolates based on MSP 1 and MSP 2 genes from Kolla Shele area, Arbaminch Zuria District, southwest Ethiopia
... and 1 unit /25 ul reaction volume of Taq DNA polymer- ase (Roche Applied Science, ...for 1 min; 50°C for 35 seconds, and 68°C for ...file 1: Figure ...falciparum msp-1 or msp-2 ...
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Imbalanced Distribution of Plasmodium falciparum MSP-1 Genotypes Related to Sickle-Cell Trait
... falciparum genotypes suggests that parasite fitness for a specific host is strain-dependent, which is consistent with our hypothesis that innate resistance might result from reduced fitn[r] ...
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Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate rich protein in rural and urban settings of southwestern Nigeria
... This study evaluated the genetic diversity of P. falciparum polymorphic markers (msp-1, msp-2 and glurp genes) in children from urban and rural communities of Oyo State, southwest Nigeria. ...
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Association of high Plasmodium falciparum parasite densities with polyclonal microscopic infections in asymptomatic children from Toubacouta, Senegal
... proteins MSP-1 (K1 parasite line) and MSP-2 (3D7), and the ring-infected erythrocyte surface antigen (RESA) was efficient against homolo- gous parasites harboring the 3D7 allelic family of ...
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Antibody responses to the merozoite surface protein 1 complex in cerebral malaria patients in India
... schizogony, MSP-1 is proteolytically processed from its ~190 kDa precursor into four major cleavage products: p83, p30, p38, and p42 [18] designated as MSP-1 83 , MSP- 1 30 , ...
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Genetic structure of Plasmodium falciparum field isolates in eastern and north eastern India
... Information on the nature and extent of genetic diversity within Plasmodium falciparum is essential in understand- ing the mechanism underlying the pathology of malaria, the acquisition of immunity, the spread of drug ...
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Genetic diversity of Plasmodium falciparum isolates from concurrent malaria and arbovirus co infections in Kedougou, southeastern Senegal
... all msp-1 allelic families (K1, MAD20 and RO33) and IC/3D7 allelic family of msp-2 were high (>70 %) and comparable between the two study ...three msp-1 allelic types or the two ...
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Multiplex Assay for Simultaneous Measurement of Antibodies to Multiple Plasmodium falciparum Antigens
... The simultaneous measurement of Abs to multiple malarial Ags has numerous advantages, especially when only small amounts of plasma are available. The use of SAT has practical applications for characterizing the immune ...
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Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru
... Peruvian isolates are less polymorphic than African and more like Indian populations. Although, conserved epitopes were found in Peru, the observation of uneven geographic distribution of polymorphisms and the high ...
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Variation in the immune responses against Plasmodium falciparum merozoite surface protein 1 and apical membrane antigen 1 in children residing in the different epidemiological strata of malaria in Cameroon
... (diluted 1 in 100 and 1 in 1000 for MSP-1 and AMA-1, respectively) along with positive control serum (a pool of sera from 8 adults in Muyuka with life- long exposure to malaria) and 10 ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo
... considering msp-1 and msp-2 genes sepa- rately, the overall multiplicity of infection was 2 and ...multiple msp-1 or msp-2 alleles did not show any impact on the parasite ...for ...
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... in E. coli MC1061 was isolated and subsequently transformed into L. lactis . Competent cells were grown in 0.2% L. lactis in 5 ml M17 broth + 0.5% glucose overnight at 30°C. Subsequently, the culture was incubated in 25 ...
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Age specific malaria seroprevalence rates: a cross sectional analysis of malaria transmission in the Ouest and Sud Est departments of Haiti
... or MSP-1, the estimated SCR of ...or MSP-1 response, the estimated SCR decreased to ...and MSP-1, ...and MSP-1 antibody titers are different, as previously sug- ...
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Human cerebral malaria and Plasmodium falciparum genotypes in Malawi
... previous msp- 1 and msp-2 data; importantly, they demonstrate that the molecular barcodes from peripheral parasitaemia mirror those generated from sequestered parasites in various tis- sues and this ...
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