MTT assay
Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
... the MTT test, leading to incon- sistent results between cell number and cell ...the MTT assay occurred only in cultured cells, concomitantly with a decrease in the energy ...same MTT ...
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AN ASSESSMENT OF IN VITRO CYTOTOXICITY OF CYPERMETHRIN ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY
... Cypermethrin, an analogue of pyrethrins, is a pesticide used in pest control. This study dealt with the investigation of in vitro cytotoxicity of Cypermethrin on human peripheral blood lymphocytes (PBL) at 2 hrs and 18 ...
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CYTOTOXICITY STUDY OF BASELLA ALBA LEAVES EXTRACT ON BRINE SHRIMP LETHALITY BIOASSAY AND MTT ASSAY
... of MTT assay showed the maximum cell viability ...63.71%. MTT assay exhibited no lethal toxicity with the concentrations range from ...
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In vitro cytotoxicity evaluation of aqueous fruit and leaf extracts of Grewia asiatica using MTT Assay
... (MTT) assay using cell lines HEK-293 (Epidermal Kidney Cancer cell line), NCI-H522 (Cell Lung cancer cell line), HELA (Cervical Cancer cell line), Hep – 2 (Laryngeal Cancer cell line), and MCF-7 (Breast ...
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) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer
... by MTT analogue assays and to understand the major technical reasons for these ...dilution assay to objectively evaluate the chemoresponsiveness of cancer cells at different seeding ...in MTT ...
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EVALUATION OF IN VITRO CYTOTOXICITY OF DIMETHOATE ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY
... Dimethoate has been used in Jabalpur for the protection of crops from pests for last many years. Its aqueous solution is sprayed by the farmers in the fields. They come to its exposure while applying it. Hence this study ...
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IN VITRO ANTI CANCEROUS EFFECT OF THE TAXITHELIUM NEPALENSE (SCHWÄGR) BROTH STUDIED BY MTT ASSAY
... The extract of Taxithelium nepalense (Schwägr) Broth. in various solvents are screened for phytochemical compounds and the acetone extract is screened for the anti-cancerous effect on HeLa cells (cervical cancerous ...
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CHEMOPREVENTIVE EFFECTS OF PHENETHYL ISOTHIOCYANATE IN A GRAPHENE OXIDE BASED NANOCOMPOSITE AGAINST HUMAN LUNG, BREAST AND COLON CANCER CELL LINES
... using MTT assay revealed that the formulated nanocomposite did not exert any cytotoxicity (data not shown) which proves that the nanocomposite does not have toxic effects on normal ...
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ADAMTS8 targets ERK to suppress cell proliferation, invasion, and metastasis of hepatocellular carcinoma
... Notes: (A) Western blotting assays of erK, stat3, akt, p-erK, p-stat3, and p-akt protein expressions in hepg2 cells. β-actin was used as an internal loading control. (B) Western blotting assays of p53 and p21 protein ...
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Original Article ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299
... by MTT assay and colony formation assay, G0/G1 arrest of cell cycle and slightly increased proportion of apoptotic cells assessed by flow cytometry assay, decreased population of migrating ...
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INVESTIGATION ON THE IN VITRO ANTIOXIDANT, ANTIMUTAGENIC AND CYTOTOXIC POTENTIAL OF THYMUS VULGARIS L HYDRO ALCOHOLIC EXTRACT
... The cytotoxic effect of the hydro-alcoholic Thyme extract against the Hep2 and HepG2 cell lines were assessed by the MTT assay. The Thyme extract at a concentration of 50 µg/ml, remarkably reduced the ...
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DESIGN, SYNTHESIS AND EVALUATION OF ANTITUBERCULAR ACTIVITY OF AMINO AZETIDINONES FROM ISONIAZID
... ABSTRACT: Azetidin-2-one analogues are reported to exhibit various pharmacological activities like cholesterol absorption inhibitory activity, human tryptase, thrombin and chymase inhibitory activity, vasopressin V1a ...
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Evaluation of Clerodendrum inerme (L.) Gaertn. on Burkitt’s Lymphoma Cancer
... found that the hydroalcoholic extract of Clerodendrum inerme extract has cytotoxic effect which demonstrated by Brine shrimp lethality bioassay and anti-proliferative effect has shown by MTT assay and ...
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Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
... The results of MTT assay indicated that down-regula- tion of miR-27a promoted drug sensitivity of gastric cancer cells. ADR was then used as probe to evaluate drug accumulation and retention in cancer ...
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<p>Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system</p>
... MTT assay showed a decrease in cell viability in thyroid cancer line owing to the action of clove bud oil-based ...V-FITC assay pro- vides results to indicate later stage apoptosis and early ...
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In-vitro toxicity study of poly(alkylphenol) as vulcanizing agent
... As shown in Fig. 4, red cells were observed in many of the TCPS wells, but most of the cells in the TCPS were green after each of the predetermined culture times. This finding indicates that most of the cells remained ...
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Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells
... MB-231. MTT assay and Celigo analysis showed that knockdown of CCT3 inhibited the proliferation of breast cancer cells, which is consistent with reports in other ...
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<p>The Anti-Breast Cancer Effect and Mechanism of Glimepiride-Metformin Adduct</p>
... effect. MTT assay showed that GMA dose- dependently inhibited the proliferation of ER+ positive breast cancer cell line MCF-7, and triple negative breast cancer cell lines MDA-MB-231, CAL-148 and MDA-MB ...
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Deletion of low-density lipoprotein-related receptor 5 inhibits liver Cancer cell proliferation via destabilizing Nucleoporin 37
... TOPflash assay show- ing the individual roles of LRP5 and LRP6 in regulating Wnt/ β -catenin ...PCR assay (A), as well as western blot assay ...S6. MTT assay (A) and Photoimages (B) of ...
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Original Article Predictive value of miR-27b as a potential biomarker for head and neck squamous cell carcinoma
... evaluated. MTT assay, cell counting assay and invasion assay were used to assess the effects of miR-27b upregulation or downregulation on the proliferation and invasion of HNSC ...
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