Multiplex PCR assay
Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination
... a multiplex PCR assay to identify the most commonly encountered Vibrio iso- lates ...The multiplex PCR primarily targets variation in conserved house- keeping ...the multiplex ...
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Fraud Identification of Undeclared Milk Species in Composition of Sheep Yogurt and Cheese Using Multiplex PCR Assay
... Nowadays, the majority of retail yogurts and cheeses are made from cow’s milk. Pure sheep yogurt and cheese is popular because of their special taste and flavor. However, the supply of these products fluctuates with the ...
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Sequential multiplex PCR assay for determining capsular serotypes of colonizing S pneumoniae
... S. pneumoniae isolates were serotyped by Quellung reaction performed by 2 different experimenters. Thirty- three different serogroups/types were identified [30]. Thirty isolates were assigned as non-typeable pneumo- ...
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Development of a Multiplex PCR Assay for Detection and Genogrouping of Neisseria meningitidis
... the multiplex PCR-based assay. The developed multiplex PCR-based assay was tested with the 15 reference strains that belonged to the 12 ...the multiplex PCR ...
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Comparison of Multiplex PCR Assay with Culture for Detection of Genital Mycoplasmas
... Multiplex PCR assay for genital mycoplasma ...Mo.). Multiplex PCR was performed with primers specific for highly conserved regions in the urease gene of Ureaplasma ...Hot-start ...
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Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... a multiplex PCR assay that could supplant hemagglutination for Ad species ...Whereas PCR assays described for identification of Ad species and serotypes have targeted the hexon (42), pIX (3), ...
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Detection of virulence genes in Malaysian Shigellaspecies by multiplex PCR assay
... Virulence genes responsible for the pathogenesis of shig- ellosis may be located in the chromosome or on the inv plasmid borne by the organism. They are often multifac- torial and coordinately regulated, and the genes ...
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Utility of a Multiplex PCR Assay for Detecting Herpesvirus DNA in Clinical Samples
... as PCR provide the potential for rapid and sensitive detection of serious, treatable virus infections, such as those caused by the herpes group of ...specimen. PCR has the potential to overcome each of ...
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Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis
... absence of an allele, as the determinant of the difference between serovars 5 and 12 increased by typing antisera. In the United King- dom, the Porcilis Glässer vaccine cross-protects (18) between se- rovars 5 and 12, ...
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“Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence
... Ideally, any new research tool or diagnostic test should be compared against an accepted “gold standard” assay. This enables meaningful comparisons of sensitivity, specificity, and overall accuracy for the new ...
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Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus ...the multiplex PCR ...
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Original Article Development and application of a rapid molecular method for detection of fusion genes in pediatric leukemia
... Clinical specimens might inhibit enzyme-based nucleic acid amplification by containing inhibi- tors. A few studies have indicated that internal control increased the sensitivity of this assay [26-28]. However, ...
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Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes
... a multiplex PCR assay that includes all 16 SNPs among the nine genes of the innate immune system (Table ...best multiplex primers and probes pairs with the less secondary structures possible ...
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Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay
... one assay). 13 viruses in one sample was the maximum demand on the multiplex PCR ...of multiplex PCR assay, because nearly all multiplex assays performed ter- ribly ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... MQR PCR assay is able to independently quantify CMV and human DNAs in the same specimen, allowing for normalization of the CMV viral load to the measured amount of human DNA ...the assay can be ...
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Field evaluation of the photo induced electron transfer fluorogenic primers (PET) real time PCR for the detection of Plasmodium falciparum in Tanzania
... PCR assay that utilizes self-quenching primers for the detection of Plasmodium ...this PCR assay does not require internal dual-labelled probes, which are usually expensive, or use ...
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... mOTNRT-PCR assay Sensitivity analysis of mOTNRT-PCR assay was carried out using 10-fold dilutions of mixed plasmid ranging from 10 8 to 10 0 copies .../μl. Multiplex PCR kit ...
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Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real Time PCR
... a multiplex, real-time PCR assay that can identify 93% of the pathogenic mycobacteria in our laboratory in two parallel ...this assay include the Mycobacterium tuberculosis complex (MTC), the ...
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... increase in sensitivity and specificity (because of using specific probes) are advantages of this method. We did not observe non-specific interactions between primers and the human genome, HBV or other pathogens. Also, ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... as PCR (29) and reverse transcription (RT)-PCR (RT- PCR) are highly sensitive techniques for the detection of nu- cleic acid sequences from viruses and bacteria in clinical spec- imens (21, ...with ...
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