Multiplex Polymerase Chain Reaction (PCR)
Multiplex polymerase chain reaction detection of enteropathogens in sewage in Norway
... ® multiplex polymerase chain reaction (PCR) system with the gastrointestinal (GI) ...® multiplex PCR when used on collective sewage samples in outbreak ...using ...
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Differentiation of Avian Pathogenic Escherichia coli Strains from Broiler Chickens by Multiplex Polymerase Chain Reaction (PCR) and Random Amplified Polymorphic (RAPD) DNA
... using multiplex polymerase chain reaction (PCR) and random amplification of polymorphic DNA (RAPD) ...tests. Multiplex PCR was reported in a previous study [7] [10] [11] ...
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Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia
... Each PCR was conducted in a final reaction volume of 20 µL containing 3 µL of cDNA, 3 µL of 8-methoxy- psoralen (MOP) solution, 4 µL of 5×RV Primer, and 10 µL of 2×master ...The PCR protocol was 94˚C ...
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A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
... for polymerase chain reaction ...uniplex PCR protocols, followed by a single multiplex polymerase chain reaction-restriction fragment length polymorphism ...
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Point-of-care multiplex PCR promises short turnaround times for microbial testing in hospital-acquired pneumonia – an observational pilot study in critical ill patients
... It was the aim of this study to evaluate the performance and turnaround times of a new point-of-care multiplex polymerase chain reaction (mPCR) system for rapid identification of pathoge[r] ...
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Ocular mycobacteriosis—dual infection of M. tuberculosis complex with M. fortuitum and M. bovis
... The patient was started on antitubercular treatment (ATT), i.e., rifampicin 600 mg, isoniazid 300 mg, pyra- zinamide 1500 mg, ethambutol 800 mg, and pyridoxine 10 mg. In view of presence of dexamethasone implant ...
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Molecular and Microbiological Assays for Characterization of Multi-Drug Resistant Tuberculosis (MDR-TB) in Clinical Samples- Clinical Relevance for the Disease Diagnosis and Treatment
... by Multiplex Polymerase Chain Reaction method - Clinical implementation for the Disease Diagnosis and ...and PCR set up for multiplex PCR, detection of amplicons using ...
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Multiplex PCR for the simultaneous detection of the Enterobacterial gene wecA, the Shiga Toxin genes (stx1 and stx2) and the Intimin gene (eae)
... Several multiplex polymerase chain reaction (PCR) methods for the detection of virulence factors and sero- group markers associated with human gastrointestinal diseases have been ...
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Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription polymerase chain reaction
... the PCR annealing temperature to 62 °C, which produced amplicons of the appropriate size for each genotype target without extraneous banding ...the multiplex PCR. In the future, a multiplex ...
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Multiplex real-time reverse transcription polymerase chain reaction for diff erential detection of H5, N1, and N8 genes of highly pathogenic avian infl uenza viruses
... transcription polymerase chain reac- tion (RRT-PCR) is a powerful tool for the sensitive and specifi c detection of viral nucleic acids in clini- cal samples, and it is faster and more specifi c than ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for the simultaneous and the vector species Culex ...
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DETECTION OF HUMAN INTESTINAL PROTOZOA BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City
... A multiplex PCR reduces labour time, reagent costs and the risk of cross-contamination, and offers the possibility of detecting multiple targets in a single multiplex ...A multiplex PCR ...
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DETECTION AND DIFFERENTIATION OF HUMAN PROTOZOAN PARASITES IN STOOL SPECIMENS BY USING MULTIPLEX ALLELE SPECIFIC POLYMERASE CHAIN REACTION (MAS-PCR) In New Damietta City, Egypt
... .The Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) assay for the detection of Cryptosporidium, Dientamoeba, ...the multiplex allele specific polymerase ...
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MOLECULAR ANALYSIS OF MLL GENE REARRANGEMENTS IN CHILDHOOD ACUTE LEUKEMIA: A HOSPITAL BASE STUDY
... Paired multiplex reverse-transcriptase polymerase chain reaction (PMRT-PCR) technique was performed for molecular ...that multiplex PCR as a sensitive molecular technique ...
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Detection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
... a multiplex allele-specific polymerase chain reaction (MAS)-PCR assay to simultaneously detect mutations in the first and third bases of the embB gene codon 306 ATG in ethambutol (EMB) ...
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Frequency of BCR-ABL Fusion Transcripts in Iranian Azeri Turkish patients with Chronic Myeloid Leukemia
... Should polymerase chain reaction analysis to detect minimal residual disease in patients with chronic myelogenous leukemia be used in clinical decision making? ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... nested polymerase chain reaction (PCR) technique shows promising sensitivity for the detection of malignant tumor cells (Mou et ...nested PCR could further improve sensitivity to liver ...
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5′ Nuclease PCR Assay To DetectYersinia pestis
... conventional PCR assays reported by Hinnebusch and Schwan (7, 8), 90% of engorged fleas were positive; preparation of the fleas was simple, involving homog- enization in 50 ml of brain heart infusion broth, ...
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Metabolic characteristics and genomic epidemiology of Escherichia coli serogroup O145 : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
... Table 2.1: PCR primer sequences and resulting amplicon lengths ........................ 22 Table 2.2: Reference genomes used to identify LEE pathogenicity island integration sites. ...
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SCIENTIFIC CONCEPTS OF POLYMERASE CHAIN REACTION (PCR)
... Therefore, knowing of the sequence of one strand will reveal the sequence of the complementary strand. Amplification is necessary because there are 3.9 billion bases, and although there is a lot of total DNA, there is ...
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