mutant p53 gain of function
ERβ decreases the invasiveness of triple-negative breast cancer cells by regulating mutant p53 oncogenic function
... on mutant p53 signaling since p53 is frequently mutated in TNBC and mutant p53 proteins promote tumor metastasis [10, 12, 17, ...of mutant p53 ...
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Mutant p53–associated myosin X upregulation promotes breast cancer invasion and metastasis
... and mutant p53 are coexpressed in breast ...has gain-of-function properties ...strong p53 immunostaining is associated with the presence of mutant TP53 (36, ...and p53 ...
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Overcoming the Dominant Negative Effect of Mutant p53+/R172H Mice.
... Mutant p53 proteins accumulate in tumors, function as dominant negative inhibitors to block wild type p53 activity and some mutant p53 proteins display ...
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RECQ1 expression is upregulated in response to DNA damage and in a p53 dependent manner
... in p53-deficient tumor cells that are much more reliant than normal cells on pathways that resolve replication ...the mutant p53-expressing MDA-MB-231 cells and this induction is lost upon targeted ...
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Mutant p53 accumulates in cycling and proliferating cells in the normal tissues of p53 R172H mutant mice
... missense p53 R172H mutation which corresponds to the p53 R175H hot spot mutation in human tumours, and is associated with Li- Fraumeni Syndrome [23, ...the p53 DNA-binding domain results in a protein ...
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Gain-of-function p53 mutants have widespread genomic locations partially overlapping with p63.
... -175 p53 GOF mutant ...the mutant p53 alleles and the cellular context used here are different: this raises the possibility that the set of TFs tethering mutp53 might be specific for mutp53 ...
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Characterization of a new mouse p53 variant: loss-of-function and gain-of-function
... reported p53 variant was not able to regu- late p21 or other target genes because of the loss of its transactivation ...wild-type p53 for a dominant-negative ...new p53 variant could suppress the ...
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Gain of function mutant p53 proteins cooperate with E2F4 to transcriptionally downregulate RAD17 and BRCA1 gene expression
... Figure 7: Reduced expression of DNA repair genes correlates with mutant p53 expression in cancer patients. (A, B) BRCA1 and RAD17 mRNAs were analyzed by RT-qPCR on a group of 63 HNSCC tumor samples and on ...
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Suppression of gain-of-function mutant p53 with metabolic inhibitors reduces tumor growth in vivo
... follows: p53, β-actin, p70S6K, MAPK/ ERK kinases 1 and 2(MEK)1/2, ERK, HSP90 and HSC70 from Santa Cruz (Dallas, TX); and PARP, cyclin D3, CDK4, p-AMPK, p-raptor, p-p70S6K, p-4EBP1, cleaved PARP, p27, p-MEK1/2, ...
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The Gain of Function of p53 Mutant p53S in Promoting Tumorigenesis by Cross-talking with H-RasV12
... criminate between different p53 responsive elements [17]. By assessment of its transactivation capacities in yeast assays [7], N239S was classified as a non-functional mutant. The computational analysis and ...
10
An inducible mouse model for skin cancer reveals distinct roles for gain and loss of function p53 mutations
... 5, the presence of the neo cassette in intron 4, and primers R172H reverse and R172H forward (PM1 and PM2, respectively; see Methods), which were used for PCR analysis of the p53 allele. The neo cassette prevents ...
10
The mutant p53 ID4 complex controls VEGFA isoforms by recruiting lncRNA MALAT1
... splicing factor SRSF1 bridges MALAT1 to mutant p53 and ID4 proteins in breast cancer cells... Mutant p53 and ID4 delocalize MALAT1 from nuclear speckles and favor its association with.[r] ...
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Activation of the human immunodeficiency virus type 1 long terminal repeat by transforming mutants of human p53.
... Since the promoters that are activated by a mutant p53 have specialized transcription factor-binding sites, we tested whether the mutant p53-281G can activate a minimal promoter with a T[r] ...
8
Drosophila Gain-of-Function Mutant RTK Torso Triggers Ectopic Dpp and STAT Signaling
... Overactivation of receptor tyrosine kinases (RTKs) has been linked to tumorigenesis. To understand how a hyperactivated RTK functions differently from wild-type RTK, we conducted a genome-wide systematic survey for genes ...
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Tumor driven by gain-of-function HER2 H878Y mutant is highly sensitive to HER2 inhibitor
... Due to its high rate of deregulation (either overexpression or mutation) in various tumor types, HER2 is among the most intensely investigated drug targets. Mutations of HER2 within kinase domain are roughly grouped into ...
12
Gain of function mutant of angiotensin II receptor, type 1A, causes hypertension and cardiovascular fibrosis in mice
... this mutant receptor expression in homozygous mice recapitulate its in vitro characteristics: the pressor response is more sensitive to Ang II and longer ...
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Quaternary structure of the specific p53 DNA complex reveals the mechanism of p53 mutant dominance
... bound p53 particles, we used a DNA target with several contiguous p53 binding ...consecutive p53 CON REs (Figure ...revealed p53–DNA complexes with 3–4 protein particles attached to DNA ...
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CP 31398 restores mutant p53 tumor suppressor function and inhibits UVB induced skin carcinogenesis in mice
... Effects of CP-31398 treatment on cell-cycle progression in human epi- dermoid carcinoma A431 cells. (A) CP-31398 induces cell-cycle arrest in A431 cells. (B) Western blot showing the expression of p53 and its ...
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DNA-Mediated Oxidation of Transcription Factor p53
... factor p53 is warmly referred to as the guardian of the genome, since it plays a critical role in sensing cellular stress and appropriating an according ...by p53 it must contain a p53 response ...
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<p>6,7-Dihydroxy-2-(4′-hydroxyphenyl)naphthalene induces HCT116 cell apoptosis through activation of endoplasmic reticulum stress and the extrinsic apoptotic pathway</p>
... well in six-well plates, incubated overnight, and then treated with PNAP-6 at different concentrations for 24 or 48 hours or with 20 μ M PNAP-6 at different time intervals. The cells were washed with PBS and lysed in ...
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