Myosin light-chain kinase
Integrin β1, myosin light chain kinase and myosin IIA are required for activation of PI3K-AKT signaling following MEK inhibition in metastatic triple negative breast cancer
... β1, myosin light chain kinase (MLCK), and myosin IIA to mediate activation of PI3K-AKT and sustained motility when the ERK signaling pathway is ...
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Altered expression levels of occludin, claudin-1 and myosin light chain kinase in the common bile duct of pediatric patients with pancreaticobiliary maljunction
... Methods: Specimens of the common bile duct were collected from 12 pediatric patients with PBM and 10 non-PBM controls. The expression of the tight junction-associated proteins occludin and claudin-1 in the epithelium was ...
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Myosin Light Chain Kinase Knockout Improves Gut Barrier Function and Confers a Survival Advantage in Polymicrobial Sepsis
... ring. Myosin light chain kinase (MLCK) phosphorylates the myosin regulatory light chain, resulting in contraction of the actin-myosin ring, subsequently increasing ...
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Myosins 1 and 6, myosin light chain kinase, actin and microtubules cooperate during antibody-mediated internalisation and trafficking of membrane-expressed viral antigens in feline infectious peritonitis virus infected monocytes
... that myosin light chain kinase (MLCK) and myosin 1 are crucial for the initiation of the ...and myosin 1 co-localise with antigens even before internalisation ...started. ...
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Role of myosin light chain kinase in intestinal epithelial barrier defects in a rat model of bowel obstruction
... detail. Myosin light chain kinase (MLCK) is actively involved in the contraction of epithelial perijunctional actinomyosin ring and thereby increases paracellular ...
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Inverse relationship of Rho kinase and myosin-light chain kinase expression in the aging human detrusor smooth muscle
... Rho kinase (ROCK) and myosin-light chain kinase (MLCK) in the aging human bladder and could demonstrate that ROCK contribution to detrusor contractility was positively correlated with ...
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Relative affinity constants by electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry: calmodulin binding to peptide analogs of myosin light chain kinase
... ABSTRACT Synthetic RS20 peptide and a set of its point-mutated peptide analogs have been used to analyze the interactions between calmodulin (CaM) and the CaM-binding sequence of smooth-muscle myosin light ...
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Epithelial myosin light chain kinase–dependent barrier dysfunction mediates T cell activation–induced diarrhea in vivo
... epithelial myosin II regulatory light chain (MLC), which has been correlated with tight junction regulation in vitro, increased abruptly after T cell activation and coincided with the development of ...
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Slit and Netrin-1 guide cranial motor axon pathfinding via Rho-kinase, myosin light chain kinase and myosin II
... and myosin light chain kinase (MLCK), which regulate myosin II activity, controlling actin retrograde flow in the growth ...and myosin II are required for Slit and ...
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A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart
... myocardium. Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v), which is involved in the development of human cardiomyopathy, is an important structural protein that affects ...
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Protein Kinase A Regulates beta-2 Integrin Avidity Activation and Subsequent Neutrophil Activation via Modulation of Myosin Light Chain Kinase.
... affected by other kinases and by myosin light chain phosphatase (Howe 2004, Smith et al 2003, Chew et al 1998, Lamb et al 1988, Blue et al 2002). Western blot analysis of lysates of PMN treated with ...
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Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin 2 and myosin light chain kinase 2
... MLCK2, a calcium/calmodulin (cam) dependent enzyme, which expresses in adult skeletal muscle, was expressed at a significantly higher level in sera from pan- creatic cancer than in the non-cancer-bearing control sera ...
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Contractile elements and myosin light chain phosphorylation in myometrial tissue from nonpregnant and pregnant women
... of myosin and actin per milligram of protein and per tissue cross-sectional area was similar between myometrium of nonpregnant and pregnant ...addition, myosin light chain kinase and ...
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The effect of histamine and cyclic adenosine monophosphate on myosin light chain phosphorylation in human umbilical vein endothelial cells
... phosphorylated myosin light chain indicated that myosin light chain kinase phosphorylated MLC20 in HUVECs under basal, cAMP-, and histamine-stimulated ...
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Effects of high glucose on human umbilical vein endothelial cell permeability and myosin light chain phosphorylation
... activation, which could affect MLC phosphorylation lev- els. The results showed that Y27632 and dnRhoA alone could induce a small amount of MLC phosphoryla- tion, but the mechanisms responsible for this phenom- enon are ...
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Attenuated platelet aggregation in patients with septic shock is independent from the activity state of myosin light chain phosphorylation or a reduction in Rho kinase-dependent inhibition of myosin light chain phosphatase
... The global impact of septic shock is profound; in 2012, the Surviving Sepsis Campaign Guidelines Committee stated that severe sepsis and septic shock affect ‘ millions of people around the world, killing one in four (and ...
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Shedding light on local kinase activation
... one the donor overlaps with the absorption spectrum of the other the acceptor. The elegant but simple logic behind ‘biosensors’ is to design a lightactivated protein so that it responds to an altered biochemical ...
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IIb or not IIb? Regulation of myosin heavy chain gene expression in mice and men
... Figure 3 Myosin heavy chain IIb isoform (MyHC-IIb) promoter responsiveness and messenger RNA (mRNA) expression in human muscle cells. (A) The mouse and human 1.0 kb MyHC-IIb promoter regions were equally ...
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Aberrant immunoglobulin synthesis in light chain amyloidosis Free light chain and light chain fragment production by human bone marrow cells in short term tissue culture
... with light chain amyloid (AL) deposition were examined by biosynthetic labeling ...monoclonal light chain (L-chain) synthesis even in those patients whose serum or urine contained no M ...
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Diagnosis and monitoring for light chain only and oligosecretory myeloma using serum free light chain tests
... The first commercially available sFLC assay (Freelite®) utilises sheep polyclonal antibodies highly specific for the epitopes exposed on LC that are not bound to heavy chain (Bradwell, et al 2001). The greater ...
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