Myxoma virus - classification, properties and replication
Role of the Serine-Threonine Kinase PAK-1 in Myxoma Virus Replication
... signaling properties of the various 3T3 ...MV replication occurred after virion binding, entry, and early gene expression rather than by any reduction in receptor-me- diated binding or ...
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<p>Impact of Induced Syncytia Formation on the Oncolytic Potential of Myxoma Virus</p>
... Syncytia Forming MYXV ’ s Maintain Their Interferon Mediated Cancer Tropism It has been previously shown that fusogenic oncolytic viruses can induce syncytia formation while still retaining their cancer- speci fi c ...
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Pathogenesis of myxoma virus
... between virus titres in the primary inoculation site in any of the ...attenuated virus and in rabbits with a degree of genetic ...delayed, virus does reach distal tissues but replicates to lower ...
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A Whole-Genome RNA Interference Screen for Human Cell Factors Affecting Myxoma Virus Replication
... of myxoma virus in human ...is, virus growth was stimulated by siRNAs targeting 711 genes and was inhibited by 333 siRNA ...of virus growth, and it suggests that organisms might encode a great ...
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Genetic Variability of Myxoma Virus Genomes
... Because of this deletion, M148R is severely truncated, and M149R, M150R, M151R, M152R, M153R, M154L, M156R, and M008.1R are deleted completely. All encoded proteins play a role in immunomodulation (21–28). ...
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Pharmacological Manipulation of the Akt Signaling Pathway Regulates Myxoma Virus Replication and Tropism in Human Cancer Cells
... MYXV replication in nonpermissive type III human cancer cells, only okadaic acid and endothall were found to be effec- tive at rescuing virus ...MYXV replication was correlated with elevated levels ...
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Deletion analysis of two tandemly arranged virulence genes in myxoma virus, M11L and myxoma growth factor.
... Deletion of M1lL had a profound effect on the ability of MYX to propagate in primary spleen cell cultures (Fig. 8), and the in vitro growth properties in spleen cell cultures of vMYX-Mll[r] ...
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Myxoma Virus Encodes an α2,3-Sialyltransferase That Enhances Virulence
... the myxoma virus EcoRI-G2 fragment located 24 kb from the right end of the 163-kb genome has been ...the myxoma virus genome encodes homologs of the vaccinia virus genes A51R, A52R, ...
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Structural studies of myxoma virus antagonists of innate immunity
... restricts replication in rabbit cells, but doesn’t affect viral replication in certain human cancer or primate cell lines 36 ...KO virus acting instead as a protective, non-replicating vaccine 36 ...
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RNA Polymerase Mutations Selected during Experimental Evolution Enhance Replication of a Hybrid Vaccinia Virus with an Intermediate Transcription Factor Subunit Replaced by the Myxoma Virus Ortholog
... the myxoma (MYX) virus ortholog exhibited de- creased ...hybrid virus with the MYXA8 gene, plaque sizes and virus yields ...of virus pop- ulations at passage 10 revealed high ...
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Development of a poxvirus vaccine : the myxoma virus/European rabbit model
... These are plagued with problems including a high incidence of side effects such as vaccine- induced disease, transmissibility and poor efficacy. This project has utilised the infection of European rabbits (Oryctolagus ...
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M135R Is a Novel Cell Surface Virulence Factor of Myxoma Virus
... disease virus (YLDV 136), but not Yaba monkey tumor virus (24), of the yatapox- ...fibroma virus, has a fragmented version of even this truncated open reading frame ...MV, virus ...
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Construction, replication, and immunogenic properties of a simian immunodeficiency virus expressing interleukin-2.
... The virus reisolated from one animal (7741-IL2) pro- duced IL-2 until at least 16 weeks ...SIV-IL2 virus, the percentage of CD29 1 CD4 1 cells was determined, since a drop in this population is an early ...
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Properties and use of novel replication competent vectors based on Semliki Forest virus
... The lack of detectable IRES-derived marker expression in the case of SFV4-d1EGFP-MEMCV and SFV4-d1EGFP- MCR could not be attributed to the position of IRES with respect to the native SG promoter, the length of spacer ...
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Myxoma Virus M083 Is a Virulence Factor Which Mediates Systemic Dissemination
... removing the majority of the m083l ORF, mRNA from the m083l gene itself was observed only in cells infected with MYXV Lausanne and vGFP (Fig. 1C). MYXV lacking M083 displays normal infection and morphogenesis. To deter- ...
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Oncolytic myxoma virus synergizes with standard of care for treatment of glioblastoma multiforme
... Infection was then monitored for differences in rate of infection, titer and rate of spread. Cel- lular death was measured by MTT assay and Caspase-3 colorimetric assay. Patient biopsies were harvested and treated under ...
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Myxoma virus therapy of human embryonal rhabdomyosarcoma in a nude mouse model
... wild-type virus, enable easy detection, and/or enhance immunogenicity in ...the virus to enhance apoptosis; however, this may not allow for maximal viral replication in ...
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The current status and future directions of myxoma virus, a master in immune evasion
... It has become clear over the years that developing effective vaccines and efficient vaccination strategies for rabbits are not as straightforward as originally thought. The major problems encountered are balancing the ...
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Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses
... of replication-competent recombinant viruses is their potential use in vivo, where multiple replication cycles are ...systemic virus replication in mice is transient and may only occur at ...
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Functional Properties of the Human Cytomegalovirus IE86 Protein Required for Transcriptional Regulation and Virus Replication
... Our analysis using transient transfection assays suggested that mutations at either amino acid 535 or 544 inhibited the ability of IE86 to activate the UL54 and UL112-113 promoters (Fig. 1). Since the products of both of ...
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