N/C-terminal heptad repeat
Conserved Salt Bridge between the N- and C-Terminal Heptad Repeat Regions of the Human Immunodeficiency Virus Type 1 gp41 Core Structure Is Critical for Virus Entry and Inhibition
... an N-terminal heptad repeat (NHR) (also called HR1), a C-terminal heptad repeat (CHR) (also called HR2), and a tryptophan-rich ...A C peptide known as T20 ...
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Six helix bundle completion in the distal C terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection
... Background: The native pre‑fusion structure of gp120/gp41 complex of human immunodeficiency virus type 1 was recently revealed. In the model, the helices of gp41 (α6, α7, α8, and α9) form a four‑helix collar underneath ...
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Mutations of Glu560 within HIV 1 Envelope Glycoprotein N terminal heptad repeat region contribute to resistance to peptide inhibitors of virus entry
... to N‑ and C‑terminal heptad repeat regions (HR1 and HR2, respectively) of gp41 can inhibit HIV‑1 infection in a dominant negative manner by interfering with refolding of the viral HR1 ...
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Nonneutralizing Antibodies Induced by the HIV-1 gp41 NHR Domain Gain Neutralizing Activity in the Presence of the HIV Fusion Inhibitor Enfuvirtide: a Potential Therapeutic Vaccine Strategy
... FIG 2 Schematic representation of the cooperative effect of combining enfuvirtide and NHR-specific antibodies in activity against gp41-mediated membrane fusion. Once gp120 binds to CD4 and a coreceptor such as CCR5, the ...
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Heptad Repeat 2-Based Peptides Inhibit Avian Sarcoma and Leukosis Virus Subgroup A Infection and Identify a Fusion Intermediate
... distinct heptad repeat domains within the transmembrane ...two heptad repeat domains of the avian sarcoma and leukosis virus subgroup A (ASLV-A) TM subunit of the envelope protein were ...the ...
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Mutations of Gln64 in the HIV-1 gp41 N-Terminal Heptad Repeat Render Viruses Resistant to Peptide HIV Fusion Inhibitors Targeting the gp41 Pocket
... the N-terminal region of gp41 NHR domain exhibited high resistance to T20 and low resistance to C34 but was sensitive to CP32M and C52L (Table ...the N-terminal region of the gp41 NHR ...
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The C Terminus of the B5 Receptor for Herpes Simplex Virus Contains a Functional Region Important for Infection
... Sutter, N. McLaren, and A. Oveta Fuller, J. Virol. 79:7419–7430, 2005). Heptad repeats found in the B5 C terminus are predicted to form an ␣ -helix for coiled coil ...the heptad repeat ...
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Conserved Leucines in N-Terminal Heptad Repeat HR1 of Envelope Fusion Protein F of Group II Nucleopolyhedroviruses Are Important for Correct Processing and Essential for Fusogenicity
... HR regions have been widely detected in membrane fusion proteins of paramyxoviruses, influenza virus, coronaviruses, and retroviruses and have been shown to play an essential role in viral fusion and infectivity (15, ...
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Oligomerization of the hydrophobic heptad repeat of gp41.
... hydrophobic heptad repeat of gp41 to direct protein oligomerization, we have cloned this region of HIV-1 gp41 into the bacterial expression vector pRIT2T, a plasmid which encodes the ...
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Inhibition of Murine Leukemia Virus Envelope Protein (Env) Processing by Intracellular Expression of the Env N-Terminal Heptad Repeat Region
... Immunoprecipitation. Cells were harvested 24 or 48 h after transfection, washed twice with ice-cold PBS, removed from the plates in 5 mM EDTA in PBS, centrifuged to pellet cells, and lysed with 10 volumes of prechilled ...
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The Heptad Repeat C Domain of the Respiratory Syncytial Virus Fusion Protein Plays a Key Role in Membrane Fusion
... the N terminus of the ...the N-terminal region of HRC in parainfluenza virus 5 (PIV5) is among the first sections of the protein head to move in the prefusion structure along with the fusion peptide, ...
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Peptides Trap the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Fusion Intermediate at Two Sites
... two heptad repeat motifs (HR) in the ectodomain of gp41 self-assemble into a trimer-of-hairpins (3, 7, 17, 24, ...The N-terminal HR (N-HR) folds into a parallel, trimeric coiled-coil ...
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An Antibody Directed against the Fusion Peptide of Junín Virus Envelope Glycoprotein GPC Inhibits pH-Induced Membrane Fusion
... the N- terminal portion of GPC (D260, K283, K292, H297, E300, D303, R306, and D309) were replaced with alanine, and the mutant proteins were expressed in Vero ...the N-terminal GPC ...the ...
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Functional characterization of the N-terminal and C-terminal domains of a sesame group II phytocystatin
... For over-expression of non-fusion SiCYS in E. coli, the recombinant plasmid pET-28a containing SiCYS was ob- tained from our previous work (Shyu et al. 2004). In this study, two sets of primers with the restriction ...
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Morbitzer, Robert (2013): Erstellung und Analyse von TALE-Transkriptionsfaktoren mit benutzerdefinierter Erkennungsspezifität. Dissertation, LMU München: Fakultät für Biologie
... bars. Repeat units, NLSs, and AD are displayed as black ovals, white diamonds, and white arrowheads, ...a C-terminal GFP-tag that facilitates analysis of expression in ...fi c primers (SI ...
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Potential chimeric peptides to block the SARS-CoV-2 Spike RBD
... Identification of a minimal peptide derived from heptad repeat (hr) 2 of Spike protein of sars-cov and combination of hr1- derived peptides as fusion inhibitors. Hepta[r] ...
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Requirement of the N-Terminal Activation Domain of Herpes Simplex Virus ICP4 for Viral Gene Expression
... D14 cells were plated onto 100-mm dishes. Cells were infected with the indicated viruses at an MOI of 10 PFU/cell for 1 h at room temperature. Following adsorption, fresh DMEM plus 5% FBS was added to each plate, and the ...
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Structure of the reovirus cell-attachment protein: a model for the domain organization of sigma 1.
... A region in the middle of the T1L, T2J, and T3D oi amino acid sequences in which conserved apolar residues conform to alternating repeat patterns: heptad repeats followed by alternating [r] ...
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Mutational Analysis of Residues in the Coiled-Coil Domain of Human Immunodeficiency Virus Type 1 Transmembrane Protein gp41
... Pseudovirion lysates and supernatants from pelleted pseu- dovirion stocks were also assessed for gp120 and gp160 by im- munoprecipitation with CD4-IgG (Fig. 4B and C). All mutants showed gp120 bands in both pellet ...
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Molecular basis for the control of motor-based transport of MHC class II compartments
... The N-terminal portion of ORP1L, consisting of the ankyrin repeat region (ANK; aa 1–237; ...the N-terminal Rab7- interacting ANK region of ORP1L suffi ces to specify this locali- ...
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