N-linked glycans
Comprehensive Functional Analysis of N Linked Glycans on Ebola Virus GP1
... only N-linked glycans, whereas the MLD contains both N- and O-linked ...disrupt N-linked glycan sites to gain an understanding of their role in GP structure and ...15 ...
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Stereoselective Construction of β‐Mannopyranosides by Anomeric O‐Alkylation: Synthesis of the Trisaccharide Core of N‐linked Glycans
... Abstract: A new and efficient approach for direct and stereoselective synthesis of β-mannopyranosides via anomeric O- alkylation has been developed. This anomeric O-alkylation of mannopyranose-derived lactols was ...
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Relative Quantification of N-linked Glycans in Complex Mixtures via Stable Isotope Labeling and Enhanced Analysis by Liquid Chromatography Coupled Online to Mass Spectrometry.
... of N-linked glycans from proteins allows access to the glycan’s reducing terminus where the terminal sugar resides in equilibrium between the closed hemi-acetal and reactive aldehyde forms 15 ...of ...
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<p>Increased branching and sialylation of N-linked glycans correlate with an improved pharmacokinetic profile for BAY 81–8973 compared with other full-length rFVIII products</p>
... The N-linked glycans or terminal carbohydrates were enzymatically removed from immobilized BAY 81–8973, rFVIII-FS, and rAHF-PFM, and analyzed using high- performance liquid chromatography (HPLC) to ...
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Stereoselective Construction of β‐Mannopyranosides by Anomeric O‐Alkylation: Synthesis of the Trisaccharide Core of N‐linked Glycans
... In conclusion, an efficient approach for stereoselective synthesis of challenging b-mannopyranosides has been devel- oped by anomeric O-alkylation of mannopyranoside-derived lactols. It was believed that this type of ...
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Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins
... 56). N-linked glycans of viral envelope proteins can play a role in the entry functions of these ...certain glycans located near the receptor-binding site on the top of the hem- agglutinin ...
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Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Proteins Incorporated into Infectious Virions
... 11 N-linked glycans on E1 and E2, ...some glycans play a role in protein folding, virus entry, and protection against ...these glycans in the context of authentic infectious ...at ...
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Role of N-Linked Glycans on Bunyamwera Virus Glycoproteins in Intracellular Trafficking, Protein Folding, and Virus Infectivity
... of N-linked glycans: one site (N60) on Gn and two (N624 and N1169) on ...Gc glycans remain endo H sensitive. The roles of the N-glycans in intracellular trafficking of the ...
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Redirecting Lentiviral Vectors Pseudotyped with Sindbis Virus-Derived Envelope Proteins to DC-SIGN by Modification of N-Linked Glycans of Envelope Proteins
... four N-linked glycans (9, ...process N- glycans (3). Therefore, the structures of N-glycans differ be- tween the virus produced in insect cells and that produced in ...
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N-Linked Glycans in the Variable Domain of IgG Anti-Citrullinated Protein Antibodies Predict the Development of Rheumatoid Arthritis
... These glycans are biantennary N- linked glycans that are fully galactosylated and sialylated ...domain N- glycosylation observed in IgG ACPA, which is consistent with our previous ...
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Mutational Analysis of the N-Linked Glycans onAutographa californica Nucleopolyhedrovirus gp64
... Transport of mutant gp64s to the cell surface and incorpo- ration into BV progeny. One possible explanation for the re- duced levels of infectious progeny production by the recom- binant viruses was that mutant gp64s ...
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Stereoselective Construction of β‐Mannopyranosides by Anomeric O‐Alkylation: Synthesis of the Trisaccharide Core of N‐linked Glycans
... To a solution of known compound S5 3 (0.9 g, 2.0 mmol) in 30 mL acetone and 2.0 mL water cooled at 0℃ was added N-bromosuccinimide (1.1 g, 6.0 mmol). The resulting mixture was stirred at 0 o C for 15 minutes. ...
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Role of N-Linked Glycans in a Human Immunodeficiency Virus Envelope Glycoprotein: Effects on Protein Function and the Neutralizing Antibody Response
... valently linked to the ectodomain of gp41 (29). All 24 potential N-linked sites are glycosylated on gp120 from the HIV IIIB strain expressed in Chinese hamster ovary (CHO) cells, includ- ing 13 that ...
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Role of N-linked glycans in antigenicity, processing, and cell surface expression of bovine herpesvirus 1 glycoprotein gIV.
... Radiolabelled extract of LMTK- cells infected with recombinant VVs expressing wild-type gIV in the presence e or absence a of tunicamycin and gNl b, gN2 c and gN1N2 d proteins were immun[r] ...
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Yeast-Elicited Cross-Reactive Antibodies to HIV Env Glycans Efficiently Neutralize Virions Expressing Exclusively High-Mannose N-Linked Glycans
... spacing of high-mannose glycans on Env with a high enough avidity to allow binding of native Env trimers and virus neutralization. If they are not, then a greater understanding of the factors that lead to domain ...
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Role of N-linked glycans of envelope glycoproteins in infectivity of human immunodeficiency virus type 1.
... Because recombinant proteins may display properties that are different from those of viral proteins in solution or included in the viral envelope, we have investigated here whether immun[r] ...
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Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase
... Shi X, Brauburger K, & Elliott RM 2005 Role of N-linked glycans on Bunyamwera virus glycoproteins in intracellular trafficking, protein folding, and virus infectivity.. Bowden TA, et al.[r] ...
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Alteration of liver N glycome in patients with hepatocellular carcinoma
... oligomannose N-linked glycans (high-mannose) and then transported to the ...of N-linked saccharides: high-man- nose oligosaccharides, complex oligosaccharides and hy- brid ...an ...
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Genome-Wide Analysis of Whole Human Glycoside Hydrolases by Data-Driven Analysis in Silico
... glycoside hydrolases that only degraded N-linked glycans (Figure 2d).. Our analysis showed that.[r] ...
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Suggestive Evidence for Darwinian Selection against Asparagine-Linked Glycans of Plasmodium falciparum and Toxoplasma gondii
... Toxoplasma occurs by two mechanisms. First, because Asn is encoded by AAT/C and the coding sequences of Toxoplasma are AT poor, the density of sequons in its secreted proteins (red triangles) and nucleus- encoded ...
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