N-Terminal Domain (NTD)
Cloning, expression, purification and in silico analysis of n terminal domain of pneumococcal surface protein a from all six clades
... PspA N-terminal fragments to check the conservation label of PspA across the ...of N-terminal domain in all 6 clades of PspA analyzed (Fig 2), while the conservation at secondary label ...
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The Ancestral N Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets
... ancestral N-terminal domain of Cg-BigDef1 was shown to drive bacterially triggered assembly of Cg-BigDef1 into nanonets, while Cg-BigDef1 appeared highly soluble and monomeric in ...The ...
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Dissecting the Role of the N-Terminal Domain of Human Immunodeficiency Virus Integrase bytrans-Complementation Analysis
... The N-terminal deletion mutant of HIV-2 IN (amino acids 50 to 293) was cloned by PCR using 95A2537 (59-CGGGGAACATATGCATGGGCAAGTA AATGCAGAAC-39) as the forward primer and a reverse primer identical to the T7 ...
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Characterization of an immortalizing N-terminal domain of polyomavirus large T antigen.
... 668 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary [r] ...
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An androgen receptor N terminal domain antagonist for treating prostate cancer
... AR NTD is a unique therapeutic target for CRPC and potentially other diseases of the androgen ...Functional NTD is necessary for AR transcriptional activity ...AR NTD; (c) EPI covalent binding was ...
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Characterization of the Redox Transition of the XRCC1 N-Terminal Domain.
... structural element. Peaks representing the amine and carbamate species for the calculation of the X1NTD oxidized carbimate adduct equilibrium constant are derived from 1 H- 15 N HSQCs of oxidized X1NTD, after the ...
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N terminal domain of prion protein directs its oligomeric association
... charged N terminus of the protein may be involved in directing the formation of distinct oligomeric states that are not readily accessible to the truncated molecule as residues within this region transiently ...
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Evolutionary Origin of SARS-CoV-2 (COVID-19 virus) and SARS viruses through the identification of Novel Protein/DNA Sequence Features Specific for Different Clades of Sarbecoviruses
... the N-terminal domain (NTD) of S-protein are uniquely shared by SARS-CoV-2, BatCoV-RaTG13 and most pangolin CoVs (SARS-CoV-2r cluster); another CSI supports a closer relationship of SARS-CoV-2 ...
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N terminal domain of Prion Protein directs its Oligomeric Association
... reported to be toxic to neuronal cell cultures (13, 29, 30, 66, 74, 75). The variety of cell types tested, the various preparations of recombinant PrP oligomers, and the considerable experimental variation may explain ...
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Non Stem Amino Acids Are Involved in the Phage P22 TSP NTD Stability
... the N-terminal domain (NTD, aa1-aa108), the β-helix (aa143-aa540) and the trimerization domain (aa541-aa666; see Figure ...TSP NTD binds to the crevice formed between the GP4 and ...
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Leader Proteinase of the Beet Yellows Closterovirus: Mutation Analysis of the Function in Genome Amplification
... The N-ATG-⌬N mutant was derived from the N-ATG variant via deletion of codons 58 through 442 (original numbering); mutant ORF 1a encoded only the C-terminal, proteinase do- main of L-Pro ...
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The dominant PNM2- mutation which eliminates the psi factor of Saccharomyces cerevisiae is the result of a missense mutation in the SUP35 gene.
... We also show that just as the PNM2 phenotype requires only an intact N-terminal domain, so SAL3 function only re- quires translation of an intact C-terminal domain and [r] ...
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Characterization of the Bacillus subtilis Penicillin Binding Protein PBP4
... Thermal denaturation analyses of PBP4* and its N-terminal domain (PBP4* ΔCTD) were performed by DSF (Differential Scanning Fluorimetry) using the Prome- theus NT.48 (NanoTemper Technologies), a ...
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Intra-ER sorting of the peroxisomal membrane protein Pex3 relies on its luminal domain.
... The N-terminal 45 amino acid residues of ScPex3 contain the short luminal (17 amino acids) segment and the transmembrane segment followed by a few amino acid ...Pex3 domain has been shown to be ...
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The Hypervariable Domain of the Murine Leukemia Virus Surface Protein Tolerates Large Insertions and Deletions, Enabling Development of a Retroviral Particle Display System
... the domain was removed, and tolerance for small insertions was ...hypervariable domain consists of 41 amino acids, residues 244 to 284. The N-terminal section of this domain appeared to ...
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The RNase III Family: A Conserved Structure and Expanding Functions in Eukaryotic dsRNA Metabolism
... Rnt1p functions as a 108 kDa homodimer (Lamontagne et al., 2000; Nagel and Ares, 2000) that forms through dimerization signals located at the N-terminal domain and the dsRBD. Rnt1p cleaves RNA by ...
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The N Terminal Membrane Spanning Domain of the Escherichia coli DNA Translocase FtsK Hexamerizes at Midcell
... The N-terminal membrane-spanning domain of FtsK is required for cell division, whereas the C-terminal domain is a fast double- stranded DNA (dsDNA) translocase that brings the ...
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Retroviral retargeting by envelopes expressing an N-terminal binding domain.
... the N-terminal insertion of polypeptides able to bind either Ram-1 phosphate transporter (the first 208 amino acids of amphotropic murine leukemia virus surface protein) or epidermal growth factor receptor ...
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Crystal Structure of the Oligomerization Domain of the Phosphoprotein of Vesicular Stomatitis Virus
... tral domain positions these two functional domains at the opposite ends and brings multiple copies of P (such as a tetramer) in close proximity through ...central domain interacts directly with any other ...
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The bovine papillomavirus type 1 E2 transactivator and repressor proteins use different nuclear localization signals.
... The E2 gene of bovine papillomavirus type 1 encodes several proteins that are required for regulation of transcription of early viral promoters and for replication of the viral genome (reviewed in references 12, 15, and ...
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