N2a cell
Activated β-catenin Forces N2A Cell-derived Neurons Back to Tumor-like Neuroblasts and Positively Correlates with a Risk for Human Neuroblastoma
... of N2A cell-derived neurons ...of N2A cells-derived neurons were gradually changed as doses of BIO increased ...presented cell death-like phenotype as nucleus was broken down when high doses ...
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Entry of Challenge Virus Standard (CVS) 11 into N2a cells via a clathrin mediated, cholesterol , dynamin , pH dependent endocytic pathway
... in N2a cells. a Quantification of cytotoxic effects of M β CD on N2a cells ranging from 0 to 20 mM was examined by MTT ...b N2a cells were pretreated with increasing concentrations (0 mM, ...e ...
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Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains
... drugs that indirectly targeted cholesterol esterification by affecting steps of cholesterol metabolism/trafficking other than biosynthesis, were associated with a reduc- tion of the CE pool and selective anti-prion ...
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Cultured Cell Sublines Highly Susceptible to Prion Infection
... prion-infected cell lines. For unknown reasons, most cell lines are resistant to prion infection (4, ...Prion-infected N2a cells are traditionally re- ferred to as ...hypothalamic cell line, ...
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Cellular Chaperonin CCTγ Contributes to Rabies Virus Replication during Infection
... mouse N2a cells as a ...mouse N2a cells infected with RABV or cotrans- fected with RABV genes encoding nucleoprotein (N) and phosphoprotein (P), confocal microscopy demonstrated that upregu- lated cellular ...
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Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells
... Fig. 3 As- and LbCpf1 nucleases can efficiently cleave randomly picked targets. Percentages of GFP positive cells counted above the background level, resulted by the action of various nucleases. Eleven randomly picked ...
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Evaluation of the cytotoxic potential of Annona muricata Linn leaf extract against N2a-neuroblastoma cells
... programmed cell death, was focused in the present ...over N2a cell lines (neuroblastoma cells) was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase ...
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Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity
... dopaminergic N2A cells remains to be deci- ...dopaminergic N2A cells induces an NRF2 antioxi- dant response, and blocks the toxicity of A β oligo- mers in neuronal cultures ...in cell cultures, and ...
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Inhibition of Protease-Resistant Prion Protein Formation in a Transformed Deer Cell Line Infected with Chronic Wasting Disease
... mouse N2a cells for five passes as measured by detection of PrP-res by immunoblotting (data not ...mouse N2a cells can be rapidly lost in some lots of OptiMEM and occasional lots of Invitrogen-certified FBS ...
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Detergent resistant membrane-associated IDE in brain tissue and cultured cells: Relevance to Aβ and insulin degradation
... Nearly confluent N2a cells grown in 100 mm dishes were washed in PBS/9% sucrose/protease inhibitor cocktail. Cellular homogenates were obtained after 10 strokes in a dounce homogenizer and 10 passages though a 22 ...
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Neurite outgrowth stimulatory effects of culinary medicinal mushrooms and their toxicity assessment using differentiating Neuro 2a and embryonic fibroblast BALB/3T3
... if any, of culinary-medicinal mushrooms. Birth defects have been identified as a growing social and healthcare issue. Congenital diseases are present in 2–3% of human newborns [11]. About 20% of the birth defects are due ...
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Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co-localization with TLR4 and nucleolin
... N2a cells were harvested for Western blot analysis at different time points after RSV infection, and uninfected normal cells served as the control group. Briefly, cells were washed with PBS and lysed in lysis ...
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Phosphorothioate Oligonucleotides Reduce PrPSc Levels and Prion Infectivity in Cultured Cells
... PS-DNA, N2a cells were pre-incubated with recHuM Fabs directed against a variety of PrP epitopes ...in N2a and ScN2a cells (15,16,46), we chose an incu- bation time and antibody concentration that would not ...
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Gaali, Steffen Paul-Günter (2012): Design and synthesis of selective ligands for the FK506-binding protein 51. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... hippocampal cell cultures from FKBP12 knockout mice FK506 retained neurotrophic activity, thus devalidating the prototypic FKBP12 as the relevant target in this ...mouse N2a cells is favored by FKBP52 ...
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Antiretroviral medications disrupt microglial phagocytosis of β-amyloid and increase its production by neurons: Implications for HIV-associated neurocognitive disorders
... ART regimens are typically comprised of two major drug classes: protease inhibitors (PIs) and inhibitors of reverse transcriptase. The latter is subdivided into nucleoside reverse transcriptase inhibitors (NRTIs) and ...
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Prion Infection of Muscle Cells In Vitro
... of cell lines susceptible to prion infection are neuronal in origin or are derived from a prion-infected ...(N2A) cell line (12, 45), a murine hypothalamic (GT1) cell line (49), a murine ...
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Inhibition of miR 128 Abates Aβ Mediated Cytotoxicity by Targeting PPAR γ via NF κB Inactivation in Primary Mouse Cortical Neurons and Neuro2a Cells
... and N2a cells ...and N2a cells, as reflected in in- creased cell viability and reduced apoptotic rate in Aβ-treated cells following the addition of Tro ...in cell viability and a notable ...
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5,5 Dimethyl 3 (5 methylisoxazol 3 yl)cyclohex 2 enone
... Geometry . All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of ...
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A Single Codon in the Nucleocapsid Protein C Terminus Contributes to In Vitro and In Vivo Fitness of Edmonston Measles Virus
... or N2a) were established by stable transfection to constitutively ex- press ...these cell lines were performed in a manner identical to that previously described for human astroctyoma cells (ATCC HTB-17, ...
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Combination of carcinoembryonic antigen with the American Joint Committee on Cancer TNM staging system in rectal cancer: a real-world and large population-based study
... N1, N2a, and N2b stages) and C stage (C0 and C1, based on CEA levels), to compare the interaction between these two stages in patients with nonmetastatic rectal ...
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