NCI-H292 cell
MUC5B Production Is Unaffected by Akt Inhibition in Human Lung Epithelial NCI H292 Cells
... by cell attachment and Akt was investigated in human lung epithelial NCI-H292 ...low cell attachment to culture plates induced the upregulated production of both MUC5B and ...MUC5AC. ...
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Effect of mucirin, a bioactive fraction of Acorus calamus l, as mucin regulator in human lung epithelial cultured cells
... Mucus hypersecretion can be triggered by various factors, including proinflammatory cytokines TNFα, interleukin, and neutrophil elastase [18,20,29], which subsequently activates pathway involving tumor necrosis factor-α- ...
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Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells
... human NCI-H292 airway epithelial cells and the primary cultures of normal nasal epithelial cells(PNEC) in response to HDAC inhibitors with or without poly (I:C) stimulation was assessed using real-time PCR ...
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Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrin β1 in NCI H292 human lung epithelial cells
... (B) NCI-H292 cells were cultured in 96-well plates, which were precoated with PBS (CNTL) , fibronectin (FN) or BSA (BSA) with control mouse IgG (−) or anti integrin β1 inhibitory antibody ...
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Citrus jabara Extracts Suppress MUC5AC Mucin Production in Human Lung Epithelial Cells
... The treated NCI-H292 cells and primary cells were cultured on the plates were lysed in a conventional SDS sample buffer (62.5 mM Tris, 10% glycerol, 2% SDS, 0.01% bromophenol blue, pH 6.8). The samples were ...
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RNA-binding protein KHSRP promotes tumor growth and metastasis in non-small cell lung cancer
... in NCI- H1299, A549, NCI-H358 and NCI-H292 cell ...and NCI-H292) (Additional file 1: Figure S2B, ...A549 cell line (Additional file 1: Figure S3A), and then the in ...
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Plasminogen activator inhibitor-1 enhances radioresistance and aggressiveness of non-small cell lung cancer cells
... made NCI-H460 cells more resistant to radiation, we analyzed the secretome from irradiated A549 ...and NCI-H460 ...NSCLC cell lines. Expression of PAI-1 increased in irradiated A549, NCI-H358, ...
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Computational master-regulator search reveals mTOR and PI3K pathways responsible for low sensitivity of NCI-H292 and A427 lung cancer cell lines to cytotoxic action of p53 activator Nutlin-3
... A, cell cycle regulation path- way and other pathways involved in regulation of different processes related to regulation of cell ...the cell line NCI-H292, the one among two of the ...
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<p>Se-Enriched <em>Cordyceps militaris</em> Inhibits Cell Proliferation, Induces Cell Apoptosis, And Causes G2/M Phase Arrest In Human Non-Small Cell Lung Cancer Cells</p>
... on cell apoptosis of NSCLC cells, fl ow cytometry assay was con- ducted using Annexin V-FITC/PI apoptosis detection kit (Beyotime Biotechnology, Shanghai, People ’ s Republic of China) according to the manufacturer ...
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Allergen induced IL 9 directly stimulates mucin transcription in respiratory epithelial cells
... exposed NCI-H292 cells to human tracheal aspirates diluted 1:6 with culture medi- um for 8 ...cultured cell RNA and mon- itored it for the presence of MUC5AC mRNA by ...
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Effect of Mutations in the Human Immunodeficiency Virus Type 1 Protease on Cleavage of the gp41 Cytoplasmic Tail
... (A) 293T cells were transfected with WT or the R236L mutant mo- lecular clones encoding WT or PIR PR. Cell and viral lysates were prepared as described previously (28) and subjected to immunoblot- ting with the ...
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CD47 blocking immunotherapies stimulate macrophage mediated destruction of small cell lung cancer
... Three cell lines (NCI-H524, NCI-1688, and NCI-H82) exhibited significant increases in phagocytosis when treated with the CD47-blocking antibody (Figure ...One cell line, NCI-H196 ...
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Original Article Targeting ROR1 inhibits epithelial to mesenchymal transition in human lung adenocarcinoma via mTOR signaling pathway
... tumor cell proliferation and anti-apoptosis properties via over-activation of the PI3K/AKT/mTOR signaling pathway in two lung adenocarcinoma cell ...non-small cell lung cancer [42- 46], so here we ...
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Activated Raf 1 causes growth arrest in human small cell lung cancer cells
... in NCI-H209: D Raf-1:ER cells and NCI-H510: D Raf-1:ER ...suspended cell clusters (9), adhered to the plastic substratum of the tissue culture flasks, formed multinucleated giant cells, proliferated ...
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Cells Lacking the RB1 Tumor Suppressor Gene Are Hyperdependent on Aurora B Kinase for Survival.
... Using this strategy, we also identified 104 genes that were synthetic lethal with RB1, based on sgRNA depletion in the pRB-deficient cells (DOX-On pRB; No DOX) compared to the pRB-proficient cells (DOX-On pRB; +DOX), ...
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BAD overexpression inhibits cell growth and induces apoptosis via mitochondrial-dependent pathway in non-small cell lung cancer
... 1;1000, Cell Signaling Technology, Beverly, USA), Bcl-xl (#24247, 1:1000, Signalway Antibody, Pearland, USA), Bcl-2 (#24246, 1:1000, Signalway Antibody), Bax (#24250, 1:1000, Signalway Anti- body), Caspase 3 ...
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Original Article Ophiopogonin Binduceshepatocellular carcinoma MHCC97-H cell apoptosis and decreases invasion through inhibition of the JAK2/STAT3 signaling pathway
... cells. Cell viability was assessed by CCK-8 assay. Cell cycle and apoptosis were measured with flow cytometry, ...the cell invasion of MHCC97-H cells induced by ...Furthermore, cell cycle was ...
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ARHGAP30 suppressed lung cancer cell proliferation, migration, and invasion through inhibition of the Wnt/β-catenin signaling pathway
... The coding sequence of ARHGAP30 was amplified with the following primers and cloned into a linearized pLVX-puro expression vector (Clontech, Palo Alto, CA, USA): ARH- GAP30 (NM_001025598.1): forward 5 ′ -GCGAATTCATGA ...
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A map of mobile DNA insertions in the NCI-60 human cancer cell panel
... the NCI-60 cell ...unrelated cell lines (novel polymorphic); and ( iii ...one cell line ...the cell line was derived as well as somatic insertions acquired during tumor development or ...
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<p>Mitofusin1 Is a Major Mediator in Glucose-Induced Epithelial-to-Mesenchymal Transition in Lung Adenocarcinoma Cells</p>
... uenced cell survival and growth, CCK-8 assay was ...enhanced cell viability was modestly inhibited after siMFN1 transfection (Figure ...silenced, cell BrdU incorporation was reduced accordingly ...
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