NCI-H292 cells
MUC5B Production Is Unaffected by Akt Inhibition in Human Lung Epithelial NCI H292 Cells
... In the human airway, the gel-forming mucin subtypes MUC5B and MUC5AC play important roles in biophylaxis. However, the regulation of MUC5B production is less clear than that of MUC5AC. Therefore, the regulation of MUC5B ...
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Effect of mucirin, a bioactive fraction of Acorus calamus l, as mucin regulator in human lung epithelial cultured cells
... Mucus hypersecretion can be triggered by various factors, including proinflammatory cytokines TNFα, interleukin, and neutrophil elastase [18,20,29], which subsequently activates pathway involving tumor necrosis factor-α- ...
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Citrus jabara Extracts Suppress MUC5AC Mucin Production in Human Lung Epithelial Cells
... The treated NCI-H292 cells and primary cells were cultured on the plates were lysed in a conventional SDS sample buffer (62.5 mM Tris, 10% glycerol, 2% SDS, 0.01% bromophenol blue, pH 6.8). ...
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6-Mercaptopurine reduces cytokine and Muc5ac expression involving inhibition of NFκB activation in airway epithelial cells
... NCI-H292 cells, we hypothesized that 6-MP inhibits the NFκB pathway in NCI-H292 ...cells. NCI-H292 cells were serum-starved for 24 h and pretreated with 6-MP ...
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Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells
... epithelial cells. LL37 in human NCI-H292 airway epithelial cells and the primary cultures of normal nasal epithelial cells(PNEC) in response to HDAC inhibitors with or without poly ...
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Extracellular ATP is involved in dsRNA-induced MUC5AC production via P2Y2R in human airway epithelium
... in NCI-H292 cells and differentiated airway epithelial cells from COPD ...in NCI-H292 ...differentiated cells from COPD patients was significantly higher than those from ...
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Arginine deficiency augments inflammatory mediator production by airway epithelial cells in vitro
... NHBE cells, at least in vitro, are less responsive to ...between NCI-H292 and NHBE ...by NCI-H292 cells, we found no effect of poly-L-arginine on arginine uptake by NHBE ...
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Allergen induced IL 9 directly stimulates mucin transcription in respiratory epithelial cells
... and NCI- H292 ...epithelial cells had the greatest degree of immunoreactivity, but staining was also associated with the endothelium of small blood vessels and smooth mus- cle fibers in the lamina ...
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<p>Se-Enriched <em>Cordyceps militaris</em> Inhibits Cell Proliferation, Induces Cell Apoptosis, And Causes G2/M Phase Arrest In Human Non-Small Cell Lung Cancer Cells</p>
... from NCI-H292 and A549 cells treated with varying concentrations of Se-enriched Cordyceps militaris (0, ...A549 cells, and 0, 4, 8, and 12 mg/mL for NCI-H292 cells) using ...
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Shp2 positively regulates cigarette smoke-induced epithelial mesenchymal transition by mediating MMP-9 production
... Cigarette smoke (CS) is a major risk factor for the development of lung cancer and chronic obstructive pulmonary disease (COPD). Epithelial-mesenchymal transition (EMT) commonly coexists in lung cancer and COPD. CS ...
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Plasminogen activator inhibitor-1 enhances radioresistance and aggressiveness of non-small cell lung cancer cells
... made NCI-H460 cells more resistant to radiation, we analyzed the secretome from irradiated A549 ...A549 cells were assessed by a silver-staining assay, and the identities of several proteins were ...
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Original Article Targeting ROR1 inhibits epithelial to mesenchymal transition in human lung adenocarcinoma via mTOR signaling pathway
... inhibitor (Roche, Basel, Switzerland). The pro- tein concentration was confirmed in accor- dance with the BCA Protein Assay Kit (Beyotime, Shanghai, China) with bovine serum albumin utilized as a standard. After the ...
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Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells
... mesothelioma cells which did not show any increase of the relevant enzyme ...PEM-resistant cells with a microarray analysis and confirmed the expression levels with Western blot ...PEM-resistant ...
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Computational master-regulator search reveals mTOR and PI3K pathways responsible for low sensitivity of NCI-H292 and A427 lung cancer cell lines to cytotoxic action of p53 activator Nutlin-3
... Based on our drug sensitivity measurements, we chose one of the most Nutlin-3 sensitive cell lines - H1944, and two less sensitive (we call them as insenistive) cell lines - A427 and NCI-H292 for further ...
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Intrinsic fluorescence of the clinically approved multikinase inhibitor nintedanib reveals lysosomal sequestration as resistance mechanism in FGFR-driven lung cancer
... cancer cells towards nintedanib suggests that protonation-based lysosomal sequestration represents a cell-intrinsic pro- tection mechanism against this FGFR ...
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Doxycycline reverses epithelial-to-mesenchymal transition and suppresses the proliferation and metastasis of lung cancer cells
... levels. NCI-H446 and A549 cells were treated with different doses of doxycycline for 24 ...of NCI-H446 and A549 by ...both NCI-H446 and A549 cells ...in cells decreased in both ...
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TRIM11 overexpression promotes proliferation, migration and invasion of lung cancer cells
... Frozen tissue sample (about 0.1 g) was ground into powder using liquid nitrogen. Protein was extracted from frozen tissue powder and cultured cells by using RIPA lysis buffer (JRDUN Biotech., Shanghai, China) with ...
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The Expression and Regulation of Human Endosulfatase 1 (HSulf-1) in Human Lung Epithelial Cells and Fibroblasts: Its Role in Cell Proliferation, Survival, and Responses to Cadmium-induced Injury In Vitro.
... hAT2 cells were unaffected (Figure ...on H292 cells and results showed that apoptosis was induced proportional to the HSulf-1 adenovirus MOIs (Figure ...in H292 cells, more genes were ...
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Phoyunnanin E inhibits migration of non small cell lung cancer cells via suppression of epithelial to mesenchymal transition and integrin αv and integrin β3
... spindle-like cells and alteration of proteins (including the E-cadherin to N-cadherin switch; and increase of vimentin, snail, and slug) ...cancer cells, we next investigated EMT in the lung cancer ...
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Differential protein stability of EGFR mutants determines responsiveness to tyrosine kinase inhibitors
... in NCI-H3255, HCC827 and NCI-H1975 cells (Figure ...and NCI-H3255 cells EGFR is internalized within an hour of drug exposure followed by its disappearance between 3-6 h ...contrast, ...
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