negative control siRNA
miR-9 Enhances the Chemosensitivity of AML Cells to Daunorubicin by Targeting the EIF5A2/MCL-1 Axis
... eIF5A2 siRNA, and negative-control siRNA were obtained from Ribobio (Guangzhou, ...their negative control RNA were purchased from Fulengen (Guangzhou, China) (miR-9 mimic, ...
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The Cellular RNA Export Receptor TAP/NXF1 Is Required for ICP27-Mediated Export of Herpes Simplex Virus 1 RNA, but the TREX Complex Adaptor Protein Aly/REF Appears To Be Dispensable
... the negative (Neg) control siRNA pool for 48 h, Aly/REF siRNA pool for 48 h, or TAP/NXF1 siRNA pool for 24 h, followed by infection with WT KOS for 8 ...the negative siRNA ...
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Original Article Knock-down of B7-H3 by small interfering RNA promotes gemcitabine-induced apoptosis in pancreatic cell line Patu8988t
... the negative control siRNA and RNAi-mate tranfection reagents at a final concentration of 10 nmol/L, 20 nmol/L and 40 ...the negative control siRNA treated group did not show ...
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<p>Bag-1 Silence Sensitizes Non-Small Cell Lung Cancer Cells To Cisplatin Through Multiple Gene Pathways</p>
... “ control siRNA treated ” ...or negative control siRNA into A549 cells and found that Bag-1 siRNA effectively silenced Bag-1 expression in A549 cells and inhibited cell ...
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Hsp27 participates in the maintenance of breast cancer stem cells through regulation of epithelial mesenchymal transition and nuclear factor κB
... specific siRNA, the population of ALDH+ cells in AS-B145 or AS-B244 cells was significantly decreased to ...with negative control siRNA (Figure ...with negative control ...
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Original Article Silencing MAGE-C1/CT7 enhances the anticancer effects of bortezomib in multiple myeloma cell line
... in siRNA group (cells transfected with siRNA-MAGE-C1/CT7) significantly decreased and that of cells in siRNA+Bt group (cells transfected with siRNA-MAGE-C1/CT7 and treated with bortezomib) ...
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Stimulation of translation by human Unr requires cold shock domains 2 and 4, and correlates with poly(A) binding protein interaction
... after siRNA transfection, and cells were harvested 6 hours ...Unr siRNA treated ...Unr siRNA (Fig. 4c). The negative control siRNA had no effect on ...
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Resveratrol Inhibits the TRIF-Dependent Pathway by Upregulating Sterile Alpha and Armadillo Motif Protein, Contributing to Anti-Inflammatory Effects after Respiratory Syncytial Virus Infection
... alone. siRNA was used to knock down the SARM mRNA level and reduce the protein ex- pression level in resveratrol-treated ...SARM siRNA-GFP was able to function in lungs of treated mice, confocal microscopy ...
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Requirement for sphingosine 1–phosphate receptor 1 in tumor angiogenesis demonstrated by in vivo RNA interference
... 1 siRNA, but not a negative control siRNA, into established tumors inhibited the expression of S1P 1 polypeptide on neovessels while concomitantly ...
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Increased expression of SUMO1P3 predicts poor prognosis and promotes tumor growth and metastasis in bladder cancer
... We determined whether SUMO1P3 can inhibit cell apoptosis in bladder cancer. Bladder cancer 5637, T24 and UM-UC-3 cells were transfected with SUMO1P3 siRNA or negative control siRNA. The ...
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THE IMPACT OF HCV KNOCKDOWN ON STAT3 AND ADI1 EXPRESSION IN HUMAN CON1 CELLS
... star negative control siRNA (Qiagen, USA) at 10nM ...Breiefly, siRNA was diluted using serum free media and then 3ul of transfection reagent was added and incubated for 15 mins to form the ...
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Original Article Roles of MED27 in regulating glioma cells via NF-κB iNOS
... utilized siRNA approach to interfere with MED27 ex- pression, and found down- regulation of mRNA and pro- tein of MED27, accompanied with significant inhibition of glioma cell proliferation or invasion, plus ...
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<p>MicroRNA-143-3p suppresses tumorigenesis by targeting catenin-δ1 in colorectal cancer</p>
... the negative control, followed by the detection of relative luciferase activity at 48 hours after transfection with renilla luciferase activity as an endogenous ...
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Human antigen R enhances the epithelial-mesenchymal transition via regulation of ZEB-1 in the human airway epithelium
... Upon detection of the stability of ZEB-1 mRNA in cells transfected with siHuR, the half-life of ZEB-1 mRNA was significantly shortened in the HuR-silenced group versus that of the control group (Fig. 4A), suggest- ...
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Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization
... Technologies-Thermofisher), and exposed to hypoxia for 4 h where indicated. Total RNA was extracted from cells using Trizol (Life Technologies-Thermofisher), purified and enriched by Qiagen RNeasy columns for gene ex- ...
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Original Article Downregulation of CD147 inhibits skin squamous cell carcinoma cell proliferation, migration, and invasion by inhibiting phosphorylation of ERK
... and siRNA-Negative Control group (P < ...NA-Negative Control group at these three time points (P < ...and siRNA-Negative Figure ...
9
Formulation of Nonionic Surfactant Vesicles (NISV) prepared by microfluidics for therapeutic delivery of siRNA into cancer cells
... of siRNA requires the use of safe and effective delivery ...transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was ...of siRNA delivery to the ...
31
RNAi mediated knock down of Dab and Numb attenuate Aβ levels via γ secretase mediated APP processing
... trol siRNA treatment (Figure ...Numb siRNA treatment led to a 209% increase in the ratio of APP-C99 to FL-APP (Figure 4C, *p < ...to control siRNA treatment. Numb siRNA treatment ...
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Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy
... ARF6-GTP–pulldown assay. ARF6-GTP–pulldown assays were performed as previously described (14). Briefly, HRMECs were trans- fected with siRNA as described above. Cells were treated with VEGF (20 ng/ml) or vehicle ...
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<p>lncRNA RHPN1-AS1 Promotes Ovarian Cancer Growth and Invasiveness Through Inhibiting miR-1299</p>
... Figure 3 RHPN1-AS1 sponged miR-1299 in OC. (A) Diagram for the putative binding site in RHPN1-AS1 with miR-1299. The red color-highlighted sequence was the mutated site in RHPN1-AS1. (B) Luciferase reporter assay was ...
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