Negative strand
5′-Proximal Hot Spot for an Inducible Positive-to-Negative-Strand Template Switch by Coronavirus RNA-Dependent RNA Polymerase
... of negative-strand antileader-containing templates used subsequently for subgenomic mRNA ...the negative-strand antigenome of the helper ...
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Structural comparisons of the nucleoprotein from three negative strand RNA virus families
... domain that are common among the N structures of the three virus families. This motif, which we have named the (5H+3H) motif, may be a common motif responsible for encapsidating RNA by the N protein of negative ...
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Initiation of Poliovirus Negative-Strand RNA Synthesis Requires Precursor Forms of P2 Proteins
... of negative-strand RNA ...examined negative-strand synthesis in PIRCs formed in reac- tions in which 2A and 2BCP3 were expressed ...of negative-strand RNA were synthesized on ...
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Synthesis of Vesicular Stomatitis Virus Negative-Strand RNA In Vitro: Dependence on Viral Protein Synthesis
... In these experiments the amount of negative-strand RNA synthesized in the complete system consisting of nucleocapsid templates and reticulocyte lysate programmed with saturating amounts [r] ...
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3′-Terminal Sequence in Poliovirus Negative-Strand Templates Is the Primary cis-Acting Element Required for VPgpUpU-Primed Positive-Strand Initiation
... As shown earlier, some of the RNAs containing mutations in stem a were unstable in the HeLa S10 translation-replication reactions. However, it was not possible to stabilize the Rz- RNAs by adding a 5 ⬘ cap to the ...
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Role of the Alfalfa Mosaic Virus Methyltransferase-Like Domain in Negative-Strand RNA Synthesis
... wt control in a few experiments. Therefore, the experiments were repeated four or five times to obtain the data shown in Table 1; Fig. 2 represents the data from a typical experiment. Accumulation of CP in leaves ...
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Subgenomic Negative-Strand RNA Function during Mouse Hepatitis Virus Infection
... subgenomic-length negative-strand RNAs contain antileader RNA sequences (31, ...MHV negative-strand RNA synthesis is rapidly inhibited by inhibitors of protein synthesis, ...
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Conversion of VPg into VPgpUpUOH before and during Poliovirus Negative-Strand RNA Synthesis
... oligonucleotides from the 5 ⬘ end of negative-strand RNAs (Fig. 3; unpublished data). Therefore, we see no quantitative evidence of paused elongation between VPg-poly(U) at the 5 ⬘ end of ...
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Constraints of Viral RNA Synthesis on Codon Usage of Negative-Strand RNA Virus
... ABSTRACT Negative-strand RNA viruses (NSVs) include some of the most patho- genic human viruses known. NSVs completely rely on the host cell for protein trans- lation, but their codon usage bias is often ...
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Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions
... plant-infecting negative- strand RNA viruses, ...36). Negative-strand viruses there- fore have to cope with the RNA silencing defense systems of both the plant and insect ...whether ...
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Poly(A) at the 3′ End of Positive-Strand RNA and VPg-Linked Poly(U) at the 5′ End of Negative-Strand RNA Are Reciprocal Templates during Replication of Poliovirus RNA
... negative-strand RNA migrated as double-stranded RF RNA in an agarose gel (Fig. 2A, lane 1). A 2 mM concentration of guanidine HCl, a reversible inhibitor of PV RNA replication (6), prevented the ...
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Identification of signal sequences that control transcription of borna disease virus, a nonsegmented, negative-strand RNA virus.
... Our results show that the BDV genome, similar to that of other nonsegmented, negative-strand RNA viruses, contains conserved sequences that are likely to serve as signals for transcripti[r] ...
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Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.
... of negative-strand RNA ...of negative-strand RNA synthesis ...both negative- and positive-strand RNA syn- thesis when added to reactions during the first 2 min of incu- bation ...
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Reduced synthesis of Sindbis virus negative strand RNA in cultures treated with host transcription inhibitors.
... Sensitivity of viral RNA synthesis to a-A' TCA-precipitable cpm x103 Treatment None a-A 49 SYNTHESIS OF SINDBIS VIRUS NEGATIVE STRAND RNA fraction of control in: CHO cells CHOama-1 72.0 [r] ...
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Mutation of Host dnaJ Homolog Inhibits Brome Mosaic Virus Negative-Strand RNA Synthesis
... to negative-strand RNA synthesis, 1a localizes to ER membranes and recruits both positive-strand BMV RNA templates and the polymerase-like 2a protein to ER ...mutant, negative-strand ...
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Poliovirus cre(2C)-Dependent Synthesis of VPgpUpU Is Required for Positive- but Not Negative-Strand RNA Synthesis
... VPg-primed negative-strand RNA ...for negative-strand synthesis ...for negative-strand synthesis ...into negative-strand ...initiate negative-strand ...
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Transcriptional Activation of Alpha/Beta Interferon Genes: Interference by Nonsegmented Negative-Strand RNA Viruses
... for negative-strand RNA viruses (for recent reviews, see references 19 and 62) has more recently also allowed the characterization of IFN antagonists from this virus group and the study of their relevance ...
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Poliovirus CRE-Dependent VPg Uridylylation Is Required for Positive-Strand RNA Synthesis but Not for Negative-Strand RNA Synthesis
... for negative-strand RNA synthesis during viral RNA ...viral negative-strand RNA synthesis requires RNA struc- tures from both the 5⬘ and 3⬘ NTRs, the template for viral ...
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Replication of murine coronavirus defective interfering RNA from negative-strand transcripts.
... of negative-strand transcripts of MHV DI RNA would also result in ...a negative-strand RNA copy of the MHV DI RNA. When the negative-strand transcripts of the DI RNA were ...
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Requirements for Assembly of Poliovirus Replication Complexes and Negative-Strand RNA Synthesis
... of negative-strand RNA from preinitia- tion complexes formed with PV⌬P1 RNA was observed, whereas none was detected from E5PV⌬P1 (data not ...of negative-strand RNA from E5PV⌬P1 RNA might be ...
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