Nested real-time PCR
Detection and Measurement of Fungal Burden in a Guinea Pig Model of Invasive Pulmonary Aspergillosis by Novel Quantitative Nested Real Time PCR Compared with Galactomannan and (1,3) β d Glucan Detection
... fungal PCR diagnos- tics exist, including resistance of fungal cells to common DNA isolation protocols; unknown timing of DNA release; the small amount of the fungal target in clinical samples, which reaches the ...
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Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
... Figure 3 Sequences of the E6-1 and E6-2 amplicons generated from pHV101. (A) Diagram of the HPV16 long control region (LCR) and the E6 and E7 genes, whose sequences overlap partially. The E6-1 amplicon (645 bp) is ...
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Novel Technique of Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA
... single-step PCR assays revealed posi- tive results in only two “confirmed” cases ...single-step PCR technique. In contrast, both the con- ventional nested PCR and the novel QNRT-PCR ...
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Novel Wide Range Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA: Development and Methodology
... This suggests that indoctrination is particularly effective where it can exploit preexisting stereotypes and beliefs 12, leading to a “magnification effect.” Data: Modern-Day Geographica[r] ...
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A nested real time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
... the nested qPCR assay described herein with those from an in- dependent protocol [15] provides confidence that the nested qPCR assay described here generates results that may be directly comparable to other ...
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Development of Real Time PCR Assays for Genotyping of Chlamydia trachomatis
... a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... in PCR mixes at ten different ...unconcentrated. Nested PCR and a TaqMan nested real time PCR assay, targeting the 18S rRNA gene, was used to detect ...to PCR and ...
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PCR Diagnosis of Invasive Candidiasis: Systematic Review and Meta Analysis
... different PCR primers) or reference standard was assessed, we extracted data separately for each test and reference ...of PCR (real time, nested, or conventional) and methods used (DNA ...
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Comparison of Conventional, Nested, and Real Time PCR Assays for Rapid and Accurate Detection of Vibrio vulnificus
... the PCR assays, it takes at least 6 h to detect the ...C- PCR (9 h), its sensitivity is known to be better than that of C-PCR ...three PCR methods (C- PCR, N-PCR, and ...
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Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... of real-time PCR to detect and quantitate viral load, and have shown its utility as an indicator of the extent of active infection ...are time consuming and labor inten- sive and have inherent ...
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Comparison of Conventional, Nested, and Real Time Quantitative PCR for Diagnosis of Scrub Typhus
... treatment. PCR assays have been widely used for rapid identification of fastidious organisms or rickettsiae that are difficult to ...more time than Q-PCR or C-PCR, and there is a higher risk ...
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BLV CoCoMo qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm
... Results: Degenerate primers were designed from 52 individual BLV long terminal repeat (LTR) sequences identified from 356 BLV sequences in GenBank using the CoCoMo algorithm, which has been developed specifically for the ...
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A new real time PCR protocol for detection of avian haemosporidians
... our real-time protocol uses a single reaction to screen for Leucocytozoon in addition to Plasmodium and Haemoproteus ...with nested PCR a separate set of nested PCR ...
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Diagnosis of Bartonella Endocarditis by a Real Time Nested PCR Assay Using Serum
... one-step PCR amplification from such a specimen may not achieve sufficient sensitivity ...a nested PCR assay. To avoid contamination by PCR products issued from previous amplifications, this ...
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Development of a Real Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis
... Real-time PCR and nested PCR yielded identical ...by nested PCR and P. falciparum plus P. ovale infection by real- time PCR; four ...by ...
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... Multiplex PCR kit (Qiagen, Hilden, Germany) was used in the sensitivity ...plex PCR Master Mix, ...added. PCR amplifi- cation was performed on the CFX96 Touch (Bio-Rad) and the conditions were: a 15 ...
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Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels
... Examples of the typical results of the real-time PCR efficiency plot and melt curve analysis are shown in figures 1 and 2 respectively. Taken as a whole, using the manufactured molecule template, the ...
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Molecular Evolution Analysis and Geographic Investigation of Severe Acute Respiratory Syndrome Coronavirus-Like Virus in Palm Civets at an Animal Market and on Farms
... Both real-time and nested RT-PCR for the N and P genes showed that all of the palm civets and raccoon dogs sampled at the Xinyuan animal market prior to culling in January 2004 were posi[r] ...
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Real Time PCR Assay Compared to Nested PCR and Antigenemia Assays for Detecting Cytomegalovirus Reactivation in Adult T Cell Leukemia Lymphoma Patients
... over, real-time PCR revealed high viral load, even in samples assessed as negative by the antigenemia assay, and CMV DNA viral load did not differ between groups 1 and 2 ...that ...
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PCR Assays for Identification of Coccidioides posadasii Based on the Nucleotide Sequence of the Antigen 2/Proline Rich Antigen
... conventional nested PCR and a real-time LightCycler PCR assay for detection of Coccidioides posadasii DNA were designed and tested in 120 clinical ...first PCR demonstrated ...
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