Neuronal Cultures
Generation of three-dimensional human neuronal cultures: application to modeling CNS viral infections
... A-3D neuronal cul- tures relies on the ability of neural stem cells/neural pro- genitor cells to self-assemble into ...A-3D neuronal cultures is described in the Methods ...A-3D cultures was ...
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Varicella-Zoster Virus Glycoprotein I Is Essential for Spread in Dorsal Root Ganglia and Facilitates Axonal Localization of Structural Virion Components in Neuronal Cultures
... in neuronal cultures and explanted ...SH-SY5Y neuronal cell cultures with a VZV gI gene deletion strain (VZV rOka⌬gI) showed a large reduction in the percentage of cells infected and ...
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Prion Strain- and Species-Dependent Effects of Antiprion Molecules in Primary Neuronal Cultures
... example, neuronal connectivity and viability. As such, primary neuronal cultures are valuable tools routinely used for neurotrophic and antiapoptotic drug evalu- ation in neurodegenerative as well as ...
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Characterization of single non inactivating potassium channels in primary neuronal cultures of Drosophila
... Permeability and gating properties of single, non-inactivating K + channel currents in neurons of eag, a mutant which has defects in the delayed rectifier K + channel, were indistinguish[r] ...
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Daidzein induces neuritogenesis in DRG neuronal cultures
... (positive control) for 24 h. Compared to DMSO-treated control cultures, daidzen treatment significantly enhanced neurite extension and branching of large DRG neurons (Figures 1, 2A-B). Quantitative analysis of ...
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Oleic Acid and Cholinergic dysfunction in Down Syndrome Models of the Central Nervous System
... not observe an increase in ChAT expression in neuronal cultures from transgenic mice upon treatment with oleic acid. In agreement with this: the presence of oleic acid did not promote profound changes in ...
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MSK1 regulates transcriptional induction of Arc/Arg3 1 in response to neurotrophins
... Fig. 4. MSK1 promotes histone H3 phosphorylation at the Arc/Arg3.1 promoter. (A) The genomic structure of the Arc/Arg3.1 gene is shown including the positions of the SRE and Egr binding site. The positions of the three ...
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Characterization of nerve growth factor-dependent herpes simplex virus latency in neurons in vitro.
... Expression of infectious virus during the establishment of latent infections and during the reactivation of HSV after NGF deprivation from neuronal cultures inoculated with HSV in the pr[r] ...
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Ginkgolides protect against amyloid-β1–42-mediated synapse damage in vitro
... In summary we show that sub-toxic concentrations of Aβ 1–42 damaged synapses in cultured cortical and hippoc- ampal neurons. We propose that these changes model the early stages of AD, where there are significant ...
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Stem cell factor stimulates neurogenesis in vitro and in vivo
... cortical cultures prepared from embryonic mouse brains were deprived of ...immature neuronal markers and that lacked evidence of DNA damage or caspase-3 ...normoxic cultures. The SCF receptor, c-kit, ...
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Proteomic Analysis Reveals Selective Impediment of Neuronal Remodeling upon Borna Disease Virus Infection
... regulating neuronal growth and remodeling ...in neuronal plasticity, transport, and ...the neuronal growth cone have been described ...of neuronal extracts from control and BDV-infected ...
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Neuronal toll-like receptor 4 signaling induces brain endothelial activation and neutrophil transmigration in vitro
... thelial cultures induced significant activation of JNK and ERK1/2 (Figure ...glial cultures (LPS Glia CM) induced a ...LPS-treated neuronal cultures (LPS Neu CM) induced neutrophil ...
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iPSC-derived neuronal models of PANK2-associated neurodegeneration reveal mitochondrial dysfunction contributing to early disease
... To generate neurons, we subjected the iPSCs to a cortical differentiation protocol due to the very high efficiency of differentiation (>95%) [27,31]. All lines tested from the patient- derived iPSCs were able to ...
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Prion Propagation and Toxicity Occur In Vitro with Two-Phase Kinetics Specific to Strain and Neuronal Type
... strain-specific neuronal tropism and toxicity remain largely ...primary neuronal cultures to assess in vitro neuronal tropism and toxicity of different prion strains (scrapie strains 139A, ...
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Inducible Cyclic AMP Early Repressor Produces Reactivation of Latent Herpes Simplex Virus Type 1 in Neurons In Vitro
... in neuronal cultures harboring latent ...shown). Neuronal cultures infected with recombinant EGFP-expressing adenoviruses confirmed expression of the transgenes ...and neuronal ...
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Conformational templating of α-synuclein aggregates in neuronal-glial cultures
... mouse neuronal-glial cultures, we tested whether overexpression of wild-type human αS mediated by rAAV2/1 vectors and addition of amino-terminally truncated exogenous recombinant wild-type (21–140) αS ...
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The PPAR-gamma agonist pioglitazone protects cortical neurons from inflammatory mediators via improvement in peroxisomal function
... Peroxisome proliferator-activated receptor-g (PPAR-g) is a ligand-activated nuclear transcription factor [12] that is predominantly expressed in adipose tissue, the immune system [13] and also in primary rat microglial ...
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Presenilin-1 regulates induction of hypoxia inducible factor-1α: altered activation by a mutation associated with familial Alzheimer's disease
... primary neuronal cultures HIF-1a was induced normally in response to cobalt chloride but insulin induction of HIF-1a was impaired even though activation of the PI3K/Akt pathway by insulin proceeded normally ...
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Synaptic UNC13A protein variant causes increased neurotransmission and dyskinetic movement disorder
... murine neuronal cultures and functional analyses in Caenorhabditis elegans revealed that the UNC13A variant causes a distinct dominant gain of function that is characterized by increased fusion propensity ...
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Recent advances in modelling of cerebellar ataxia using induced pluripotent stem cells
... questions remain about the observed species differences in disease phenotypes and the effectiveness of potential drugs in clinical trials. To help translate research from animal models into novel treatments for ataxia ...
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