Neutrophil extracellular trap
Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro
... The aim of this study was to investigate the effects of recombinant human-soluble thrombomodulin (rTM) on lipopolysaccharide (LPS)-induced, platelet-dependent neutrophil extracellular trap (NET) ...
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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease
... Interleukin (IL)-8 is a chemoattractant and neutrophil activator and thus would be a candidate plasma factor involved in the phagocyte activation observed in BD. This possibility is favored by pieces of evidence, ...
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Superoxide induces Neutrophil Extracellular Trap Formation in a TLR-4 and NOX-Dependent Mechanism
... Oxidative stress is a defining feature of liver I/R; excess generation of ROS, such as superoxide, by liver epithelial cells, Kupffer cells and vascular endothelium results in a proinflammatory extracellular ...
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Neutrophil extracellular trap production contributes to pathogenesis in SIV infected nonhuman primates
... Neutrophils are central to the innate immune system, being involved in the defense against bacteria and fungi (1), and even against viruses, as recently reported (2). In addition to phagocytiz ing and killing ...
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A role for muscarinic receptors in neutrophil extracellular trap formation and levamisole-induced autoimmunity
... Levamisole engages neutrophil muscarinic receptors to trigger NETs. NET formation can be initiated by stimulation of various mem- brane and intracellular receptors expressed by neutrophils (e.g., TLRs, Fc γ ...
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Anti-neutrophil extracellular trap antibody in a patient with relapse of anti-neutrophil cytoplasmic antibody-associated vasculitis: a case report
... In order to determine the involvement of ANCA-NETs vicious cycle in this patient, we examined NET degradation and induction activities in sera obtained at the disease onset (Serum A; MPO[r] ...
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“In sickness and in health” – how neutrophil extracellular trap (NET) works in infections, selected diseases and pregnancy
... One of the negative effects of NET presence in extra- cellular environment is that NETs are involved in auto- immune diseases. During NET formation, citrullinated histones and antigens are released into ...
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Neonatal NET inhibitory factor and related peptides inhibit neutrophil extracellular trap formation
... (6). Neutrophil heterochro- matin decondensation is mediated by PAD4, which catalyzes con- version of histone arginines to citrullines with consequent weak- ening of histone-DNA binding and unwinding of ...
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Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis
... quantification. Neutrophil cultures were digested using micrococcal nuclease (Sigma Aldrich) to dismantle the NET ...min. Extracellular DNA was measured with a spectrofluorometer at 480-nm excitation and ...
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Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent
... For the inhibition of ERK 1/2 and p38 MAPK the follow- ing inhibitors were used respectively: UO126 (50 μM; Sigma-Aldrich) and SB 203580 (10 μM; Sigma-Aldrich). Therefore, PMN were preincubated with the inhibitors for 30 ...
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Enhanced neutrophil extracellular trap generation in rheumatoid arthritis: analysis of underlying signal transduction pathways and potential diagnostic utility
... an extracellular environ- ment by reducing the calcium requirement [33], their combination with NETs-associated PAD4 could lead to prodigious quantities of citrullinated ...the extracellular presence of ...
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Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation
... of neutrophil elastase (NE), citrulli- nated histone H3 (CitH3), and peptidylarginine deimi- nase 4 (PAD4) by confocal laser microscopy (CLSM) was performed using specimens adhered to glass cover- ...
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Neutrophil extracellular trap induced by HMGB1 exacerbates damages in the ischemic brain
... Interestingly, we found that some neutrophils in blood and the CSF of MCAO animals were already CitH3 + be- fore they infiltrated brain parenchyma. CitH3 + levels were markedly increased in peripheral blood after 12 h of ...
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A subset of patients with systemic lupus erythematosus fails to degrade DNA from multiple clinically relevant sources
... of neutrophil extracellular trap (NET) (a), primary (Prim-Nec) (b), and secondary (Sec-Nec) necrotic cell chromatin (c) in comparison to controls (Healthy ctrl) (a, n = 77; b, n = 50; c, n = ...
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Evidence of eosinophil extracellular trap cell death in COPD: does it represent the trigger that switches on the disease?
... Recently, neutrophil extracellular trap cell death (NETosis), a mechanism due to decondensation and extrusion of chromatin to form extracellular traps, has been demonstrated in ...eosinophil ...
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The Impact of HMG-CoA Reductase Inhibitors on Neutrophil Function in Patients Undergoing Colorectal Cancer Resection: An In-vitro Experimental Study
... assess neutrophil function were conducted outside of the biological context and consequently there are challenges in extrapolating the results and it must be acknowledged that they cannot be readily transposed to, ...
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ENTPD 1 disrupts inflammasome IL 1β–driven venous thrombosis
... deposition, neutrophil extracellular trap (NET) forma- tion, and canonical inflammasome activation, under control of NF-κB, the NOD-like receptor pyrin domain–containing 3 protein (NLRP3) ...
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Neutrophil Extracellular Traps: Formation and Involvement in Disease Progression
... In summary, neutrophils release NETs composed of chromatin and granule proteins. During NETosis, neutrophils throw its arsenals to trap the pathogen and kill with the help of free radicals and granule proteins. ...
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The role for neutrophil extracellular traps in cystic fibrosis autoimmunity
... In contrast, we note that another autoantibody type, ACarPA, demonstrates a moderate but note- worthy correlation with diminished pulmonary function (lower FEV1) and infection with P. aeruginosa. In contrast to anti-BPI ...
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Neutrophil extracellular traps induce aggregation of washed human platelets independently of extracellular DNA and histones
... NETs, extracellular histones, have been studied extensively and known to activate platelets and promote coagula- tion through multiple mechanisms [9, 17 – ...Furthermore, neutrophil granular enzymes that ...
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