Non-Human Genome Editing
Human germline genome editing: On the nature of our reasons to genome edit
... of human embryos would be person ...that genome editing is highly unlikely to be person affecting for the foreseeable future and, as a result, will neither benefit nor harm edited ...
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Unveiling human non-random genome editing mechanisms activated in response to chronic environmental changes.
... of non- random, “on demand” mutation, in which environmental changes through a biotic “pollutant”, drive the cells to edit specific exons of a specific eukaryotic gene, leaving the rest of the B cell genome ...
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Consecutive Genome Editing in Non-Human Primate Achieves Durable Production of Human Alpha-1 Antitrypsin and Reduction of the Native Protein
... Reduction of Cyno A1AT upon Gene Knockout Using NHP-specific Guide • ~70% decrease in cyno A1AT levels upon cyno gene knockout, stable out to 18 weeks post-treatment • No effect on human A1AT expression in animals ...
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CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications
... (three genes) was integrated into rDNA repeats clus- ter of O. polymorpha (Fig. 3c). This multi-copy pathway integration enabled a 21-fold higher resveratrol pro- duction (97 mg/L) compared to the single copy path- way ...
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A non-viral genome editing platform for site-specific insertion of large transgenes
... a non-viral genome insertion tool for safe and functional delivery of large seamless DNA cargo into the human genome that can enable novel designer cell-based ...pluripotent human ...
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Nuclease-based editing in the porcine genome : a strategy to facilitate porcine-to human xenotransplantation
... DISCUSSION A hallmark of many renal disease states is the urinary loss of essential macromolecules like serum albumin. Traditionally this has been associated with insult to the filtration barrier. In renal ...
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In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model
... the human genome using gene-editing ...and human hepa- tocytes in vivo, leading to reduced plasma concentra- tions of PCSK9 and cholesterol [22, ...by non-homologous end-joining (NHEJ) ...
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Genome editing of human pancreatic beta cell models : problems, possibilities and outlook
... or genome editing of type 2 diabetes risk alleles or deleting regulatory regions surrounding these variants in human beta cell models, followed by RNA sequencing (RNAseq) and/ or implementation of ...
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Dual-active genome-editing reagents
... and human gene therapy. Precise genome-editing requires the introduction of a specific DNA double-stand break at a locus of interest, in turn inducing host DNA repair pathways to cause gene knockout ...
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Comparative analysis of A-to-I editing in human and non-human primate brains reveals conserved patterns and context-dependent regulation of RNA editing
... regulating editing are influenced by the envir- onment and can be dynamically tuned throughout life providing adaptive ...relatively non-malleable and dictated by a molecular balance coded uniquely in each ...
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Systematic evaluation of CRISPR Cas systems reveals design principles for genome editing in human cells
... precise genome editing typically occurs at low ...cise genome edits in human cells efficiently when ssODNs are used as donor templates ...the non-target strand ...
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THE FIRST CASE oF HuMAn GEnoME EDITInG:
... The Convention prohibits the use of technologies aimed at rendering medical aid to continue a person’s family line, and choosing the gender of a future child, except for such cases where it is done to prevent inheriting ...
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CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
... lower editing efficiency com- pared with the CRISPR-Cas9 system; (2) it is difficult to deliver multi-effector components to cells or tissues; and (3) there are concerns about the immunogenicity of several bacterial ...
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Use of RNA-Protein Complexes for Genome Editing in Non-albicans Candida Species
... (CRISPR)-Cas9 genome modification systems have greatly facilitated the genetic analysis of fungal ...CRISPR-Cas9 genome editing methods designed for use in Candida al- bicans, DNAs that encode the ...
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Why are NGOs sceptical of genome editing?
... problem framing of food security; the focus on intensive agriculture and technological 38.. solutions to the problem of food security; and the motivations for adopting agricultu[r] ...
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Plant genome editing with TALEN and CRISPR
... For all HDR applications, efficiency will need to be improved. Increasing the efficiency of SSN delivery will greatly help genome editing, including HDR applica- tions. If a higher percentage of plants or ...
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Designed nucleases for targeted genome editing
... Targeted genome-editing technology using designed nucleases has been evolving rapidly, and its applications are widely expanding in research, medicine and ...this genome- modifying technology, ...
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The societal opportunities and challenges of genome editing
... literally, genome-edited animals and plants are gen- etically modified organisms — GMOs — but they differ from the controversial genetically engineered crops cur- rently under ...contrast, genome ...
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Why are NGOs sceptical of genome editing?
... argue cause systemic food vulnerability. If intensive monocultures are the problem, then 146.. genome editing is not the solution. Instead, NGO participants argued for the need to consid[r] ...
Genome Editing Approaches for Crop Improvements
... global human population is projected to exceed more than 9 billion by 2050 requiring crops with higher quality yields with multiple bene icial ...like genome editing offers the potential to transform ...
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