Nonstructural protein-1
Bovine Coronavirus Nonstructural Protein 1 (p28) Is an RNA Binding Protein That Binds Terminal Genomic cis-Replication Elements
... Nonstructural protein 1 (nsp1), a 28-kDa protein in the bovine coronavirus (BCoV) and closely related mouse hepatitis coronavirus, is the first protein cleaved from the open reading ...
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Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1
... Nonstructural protein 1 (NS1) is one of the major factors resulting in the efficient infection rate and high level of virulence of influenza A ...NS1 protein that is modified by SUMO1, except ...
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Structural Study of the C-Terminal Domain of Nonstructural Protein 1 from Japanese Encephalitis Virus
... envelope protein [E]) and seven nonstructural pro- teins, including nonstructural protein 1 (NS1), NS2A, NS2B, NS3, NS4, NS4B, and ...
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The C Terminus of the Core β-Ladder Domain in Japanese Encephalitis Virus Nonstructural Protein 1 Is Flexible for Accommodation of Heterologous Epitope Fusion
... These protein samples were prepared by either being boiled to detect mono- meric or being left unboiled to detect dimeric NS1-EV71 epitope fusion ...lanes 1 and 3), appropriate homodimers/het- erodimers in ...
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Rotavirus-Encoded Nonstructural Protein 1 Modulates Cellular Apoptotic Machinery by Targeting Tumor Suppressor Protein p53
... The versatility of the ubiquitin-proteasome system in control- ling cellular homeostasis through posttranslational protein mod- ification makes it an attractive target for viruses. Viruses manipu- late this system ...
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Delayed and highly specific antibody response to nonstructural protein 1 (NS1) revealed during natural human ZIKV infection by NS1 based capture ELISA
... The cross-reactivity of DENV-immune sera to ZIKV-NS1 is a concern for serological test in ZIKV and DENV co-circulating areas. It was reported that 36–40% plasma samples from ZIKV-naïve DENV-immune do- nors including ...
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Dependence of Coronavirus RNA Replication on an NH2-Terminal Partial Nonstructural Protein 1 in cis
... FIG 7 Point mutations that change amino acids within the WAPEFPWM domain block DI RNA replication, whereas mutations that change the codons but keep the WT amino acids enable replication. (A) Alignment of the first 62 ...
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Role for Nonstructural Protein 1 of Severe Acute Respiratory Syndrome Coronavirus in Chemokine Dysregulation
... By blocking the IKK/NF- B pathway, we demonstrated that the SARS-CoV nsp1-induced dysregulation of chemokines is mediated by NF- B activation (Fig. 4B to E). Our results were consistent with a previous report that ...
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West Nile Virus Nonstructural Protein 1 Inhibits TLR3 Signal Transduction
... of proinflammatory cytokines in response to virus infection including tumor necrosis factor alpha, IL-6, or monocyte che- motactic protein 1 (18, 21, 29, 61). However, the outcome in terms of pathogenesis ...
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Reverse Transcription PCR Enzyme Linked Immunosorbent Assay for Rapid Detection and Differentiation of Alphavirus Infections
... the nonstructural protein 1 gene, sequence-specific, biotin- labeled probes targeted against Venezuelan, eastern, and western equine encephalitis or Mayaro virus genes were used for the detection of ...
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Gain, Preservation, and Loss of a Group 1a Coronavirus Accessory Glycoprotein
... S protein? The function of gp3 is not known, but based upon its biochemical properties, it may act either in the infected cell within the compartments of the exocytotic route or in the extracellular ...
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Enzyme Linked Immunosorbent Assay Specific to Dengue Virus Type 1 Nonstructural Protein NS1 Reveals Circulation of the Antigen in the Blood during the Acute Phase of Disease in Patients Experiencing Primary or Secondary Infections
... Up to now, no particular marker has been unambiguously related to the severity of the disease. Controversial results have accumulated over the last few decades about the role of anti- bodies in dengue pathogenesis (2, ...
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Establishment of a Chimeric, Replication-Deficient Influenza A Virus Vector by Modulation of Splicing Efficiency
... export protein (NEP) expression for efficient viral ...NS1, nonstructural protein 1; NS2/NEP, nonstructural protein 2/nuclear export protein; IgK-SP, partial mouse Ig ...
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Nonstructural Protein σ1s Is Required for Optimal Reovirus Protein Expression
... NaCl, 1 mM EDTA, 1% sodium dodecyl sulfate, 1% desoxycholate, 1/100 IGEPAL ...Total protein in each sample was quantified using the DC protein assay (Bio-Rad), and equivalent ...
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H-1 Parvovirus-Associated Replication Bodies: a Distinct Virus-Induced Nuclear Structure
... major nonstructural protein (NS1) of H-1 virus which is essential for viral DNA amplification colocalized with virus-specific DNA sequences and sites of ongoing viral DNA replication in distinct ...
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Laboratory Diagnosis of Dengue Infection: Current Techniques and Future Strategies
... E protein, as its name suggests, is the principal surface protein of the virus particle (virion), and is involved in host cell attachment, typically to keratinocytes, the predominant cell type in human ...E ...
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Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival
... Bioinformatic analysis of FMDV 2C. The FMDV 2C amino acid se- quence was queried with BLAST (33) and HHpred (34) against the PDB and HHpred PDB70 database, respectively. The BLAST search of the 2C amino acids sequence ...
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In vitro assembly of infectious virions of double-stranded DNA phage phi 29 from cloned gene products and synthetic nucleic acids.
... Properties of tail protein gp9. The f 29 tail is composed of multiple subunits of gp9 proteins. A question remaining to be answered was whether the entire tail is preassembled before attaching to the DNA-filled ...
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Rotavirus Nonstructural Protein NSP3 Is Not Required for Viral Protein Synthesis
... cellular protein synthesis, while ensuring at the same time the translation of viral mRNAs, has been generated by experiments in vitro or by expression of the NSP3 gene in uninfected ...reduced protein ...
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ATP Hydrolysis and AMP Kinase Activities of Nonstructural Protein 2C of Human Parechovirus 1
... parechovirus 1 (HPEV1) is a human pathogen as- sociated with gastrointestinal and respiratory symptoms and occasionally with infections of the central nervous system ...and nonstructural proteins involved ...
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