nonstructural protein 1
Structural Study of the C-Terminal Domain of Nonstructural Protein 1 from Japanese Encephalitis Virus
... envelope protein [E]) and seven nonstructural pro- teins, including nonstructural protein 1 (NS1), NS2A, NS2B, NS3, NS4, NS4B, and ...
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Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1
... Nonstructural protein 1 (NS1) is one of the major factors resulting in the efficient infection rate and high level of virulence of influenza A ...NS1 protein that is modified by SUMO1, except ...
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Rotavirus-Encoded Nonstructural Protein 1 Modulates Cellular Apoptotic Machinery by Targeting Tumor Suppressor Protein p53
... in nonstructural protein-1 (NSP1) mutant strain A5-16, p53 degradation was not observed, suggesting a role of NSP1 in this ...p53 protein was stabilized only in the presence of MG132, ...
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Bovine Coronavirus Nonstructural Protein 1 (p28) Is an RNA Binding Protein That Binds Terminal Genomic cis-Replication Elements
... Nonstructural protein 1 (nsp1), a 28-kDa protein in the bovine coronavirus (BCoV) and closely related mouse hepatitis coronavirus, is the first protein cleaved from the open reading ...
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West Nile Virus Nonstructural Protein 1 Inhibits TLR3 Signal Transduction
... treatment, protein extracts were prepared in cell lysis buffer (300 mM NaCl, 50 mM Tris-HCl, ...and protein concentrations were determined using a detergent-compatible protein assay kit ...of ...
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The C Terminus of the Core β-Ladder Domain in Japanese Encephalitis Virus Nonstructural Protein 1 Is Flexible for Accommodation of Heterologous Epitope Fusion
... Using a direct transposon-mediated random-insertion tech- nique, YFV NS1 was previously found to have only one position, at residue 236, that could accommodate a 56-aa epitope derived from influenza virus M2e, and the ...
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Dependence of Coronavirus RNA Replication on an NH2-Terminal Partial Nonstructural Protein 1 in cis
... FIG 7 Point mutations that change amino acids within the WAPEFPWM domain block DI RNA replication, whereas mutations that change the codons but keep the WT amino acids enable replication. (A) Alignment of the first 62 ...
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Role for Nonstructural Protein 1 of Severe Acute Respiratory Syndrome Coronavirus in Chemokine Dysregulation
... S protein from the SARS-CoV was shown to induce IL-8 through the AP-1 ...nsp1 protein from two other human coronaviruses, HCoV- 229E and HCoV-OC43, were weak inducers of the chemo- kines, with ...
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Delayed and highly specific antibody response to nonstructural protein 1 (NS1) revealed during natural human ZIKV infection by NS1 based capture ELISA
... Results: The analyses of the sequential serum samples from ZIKV infected individuals showed NS1 specific Abs appeared 2 weeks later than E specific Abs. Notably, human sera from ZIKV infected individuals did not contain ...
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Reverse Transcription PCR Enzyme Linked Immunosorbent Assay for Rapid Detection and Differentiation of Alphavirus Infections
... the nonstructural protein 1 gene, sequence-specific, biotin- labeled probes targeted against Venezuelan, eastern, and western equine encephalitis or Mayaro virus genes were used for the detection of ...
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Establishment of a Chimeric, Replication-Deficient Influenza A Virus Vector by Modulation of Splicing Efficiency
... export protein (NEP) expression for efficient viral ...NS1, nonstructural protein 1; NS2/NEP, nonstructural protein 2/nuclear export protein; IgK-SP, partial mouse Ig ...
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Processing of Nonstructural Protein 1a of Human Astrovirus
... ICN Biomedicals, Irvine, Calif.) per well for 15 min. Cell lysates were prepared, and nsP1a-specific products were immunoprecipitated with MAb ␣His (Qiagen) as described previously (5). The immunoprecipitated products ...
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Theiler's murine encephalomyelitis protein 2C and its effect on membrane trafficking
... viral protein synthesis occurs at the ER. Protein 2C then redistributes to accumulate in a juxtanuclear site, possible the Golgi complex area, suggesting that this may be the viral replication ...but ...
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Low Sensitivity of NS1 Protein Tests Evidenced during a Dengue Type 2 Virus Outbreak in Santos, Brazil, in 2010
... IgG avidity. The IgG avidity test was performed on acute- and conva- lescent-phase samples that were IgG positive by use of an in-house ELISA. In brief, antigens were prepared from Aedes albopictus C6/36 cells infected ...
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Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival
... To determine whether an intact vimentin pathway is required for FMDV infection, we utilized overexpression plasmids con- taining either the wild-type or a truncated form of vimentin and showed that in the presence of ...
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Gain, Preservation, and Loss of a Group 1a Coronavirus Accessory Glycoprotein
... AY342160), 1-71 (EF056487), Insavc1 (D13096), CB/05 (DQ112226), and 229/05 (un- published data), TGEV strain Purdue (DQ811789), and FCoV-II strains 79-1683 (Y13921) and 79-1146 ...and 1-71 IGRs, an internal ...
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Enzyme Linked Immunosorbent Assay Specific to Dengue Virus Type 1 Nonstructural Protein NS1 Reveals Circulation of the Antigen in the Blood during the Acute Phase of Disease in Patients Experiencing Primary or Secondary Infections
... Up to now, no particular marker has been unambiguously related to the severity of the disease. Controversial results have accumulated over the last few decades about the role of anti- bodies in dengue pathogenesis (2, ...
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Approaches to hepatitis C treatment and cure using NS5A inhibitors
... possible AH–D1a linker conformations related to drug binding. We found the PDB ID 1ZH1 dimer template to provide better AH alignments, based on chemical genetics-derived distance constraints (J Nettles, unpublished data, ...
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Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
... positions 1 to 3385 within the genome) was reverse transcribed and PCR amplified using previously described downstream (P1Rev) and upstream (P1Fwd) primers (5=-CTT GGCCACTCAGGATGATT-3= and ...
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Role of Nonstructural Protein NS2A in Flavivirus Assembly
... Flavivirus NS2A is a small (231 amino acids), hydrophobic, multifunctional, membrane-associated protein involved in RNA replication (2, 21). NS2A binds with high specificity to the 3 ⬘ untranslated region (UTR) of ...
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