nonviral gene delivery
Polyethylenimine<sub>600</sub>-β-cyclodextrin: a promising nanopolymer for nonviral gene delivery of primary mesenchymal stem cells
... plasmid delivery agent with low toxicity and ideal transfection efficiency on primary ...the nonviral gene delivery of MSCs because of its ideal inclusion ability and proton sponge effect, and ...
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The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts
... Gene delivery approaches provide a mechanism to dir- ectly alter gene expression within a cell ...current delivery systems have limited the use of gene delivery for therapeutic, ...
11
An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives
... for gene therapy, such as for ischemic ...as nonviral gene- delivery ...vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify safety and transfection ...
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Magnetic ternary nanohybrids for nonviral gene delivery of stem cells and applications on cancer therapy
... Advanced therapeutic strategies such as sustained drug release by using GLIADEL® have been approved as a co-treatment after surgical resection with only moderate improvement [53]. Therefore, development of novel ...
13
Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications
... is nonviral gene delivery of pro-survival or anti-apoptotic factors to ...HO-1 gene that are recognized by transcription factors ...miRNA delivery can regulate gene expression ...
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An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery
... The search for efficient and safe carriers to deliver genes into cells remains a great challenge in gene therapy. Inorganic nanoparticles are especially suitable for this purpose because they can be ...
7
Gene expression profiles in primary duodenal chick cells following transfection with avian influenza virus H5 DNA plasmid encapsulated in silver nanoparticles
... effective nonviral gene delivery system against avian influenza virus (AIV) that induced cytokine expression, the hemagglutinin (H5) gene of AIV, A/Ck/Malaysia/5858/04 (H5N1) and green ...
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Degradable gene delivery systems based on Pluronics-modified low-molecular-weight polyethylenimine: preparation, characterization, intracellular trafficking, and cellular distribution
... as nonviral gene delivery systems, and a large number of different polymers and copolymers of linear, branched, and dendrimeric architectures have been tested in terms of their suitability and ...
12
Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized <em>Porphyra yezoensis</em> polysaccharide nanoparticles
... novel gene vector, cationized PPS (CPPS) nanoparticles, for the nonviral gene delivery of a plasmid DNA encoding Wnt3a into human umbilical cord mesenchymal stem cells (HUMSCs) in ...
11
Nonviral gene therapy in vivo with PAM-RG4/apoptin as a potential brain tumor therapeutic
... possible nonviral carrier for the treat- ment of malignant ...enhanced gene expression levels with low cytotoxicity in various cells making PAM-RG4 a suitable gene delivery carrier candidate ...
14
Gene therapy for C-26 colon cancer using heparin-polyethyleneimine nanoparticle-mediated survivin T34A
... the mice were sacrificed, and their tumors were excised and preserved. The ms-T34A-HPEI treatment resulted in regres- sion of tumor growth by 74.8%, 71.2%, and 66.2% com- pared with the normal saline, HPEI, and null-HPEI ...
9
PubMedCentral-PMC5512420.pdf
... Clinically, it has been suggested that the number of CXCR4 + CRC cells in a patient can be used as a biomarker to determine which patients may have a more aggressive and higher rate of liver metastasis (4, 7). Therefore, ...
27
TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
... employing gene therapy as a cure for HCC is the ability to design an efficient vector that is able to deliver therapeutic genes specifically into the cancer cells but not the surrounding benign ...the gene ...
11
Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer
... In this study, PEG-g-PEI-SPION and scFv CD44v6 were cho- sen as two essential components to construct a targeted siRNA delivery vector with MRI imaging functions. ScFv CD44v6 was generated by phage display ...
10
Multicomponent nanoparticles as nonviral vectors for the treatment of Fabry disease by gene therapy
... for gene therapy, reporter genes such as EGFP, ß-galactosidase, or luciferase plasmids are frequently used since its evaluation, both in vitro and in vivo, is simple and ...
8
Recent advances in gene therapy for lysosomal storage disorders
... for gene therapy because delivery of a single gene into a small percentage of the appropriate target cells may be sufficient to impact the clinical course of the dis- ...for gene therapy of ...
13
Feasibility of gene therapy for tooth regeneration by stimulation of a third dentition
... stated, gene therapy consists of the insertion of genes into an individual’s cells directly or indirectly with a matrix to promote a specific biological ...effect. Gene therapy can be used to induce a more ...
25
Utilization of Nonviral Sequences for Minus-Strand DNA Transfer and Gene Reconstitution during Retroviral Replication
... Using a series of MLV vectors with modified LTRs, we demonstrated that a nonviral sequence, a portion of GFP, could efficiently mediate minus-strand DNA transfer. This finding is in contrast with the in vitro ...
9
Long-term gene therapy with Del1 fragment using nonviral vectors in mice with explanted tumors
... A number of molecules have been developed as agents for cancer treatment. Unfortunately, many of these have become ineffective due to tolerance or have failed to produce satisfac- tory results. Thus, treatment with E3C1 ...
14
Aldehyded dextran and ε-poly(l-lysine) hydrogel as nonviral gene carrier
... This study has confirmed the feasibility of gene delivery using a novel self-biodegradable hydrogel. The ald-dex/PLL hydrogel showed a possibility to be used as a nonviral carrier for controlled ...
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