normal human dermal fibroblasts
Prostaglandin E2 induces Skin Aging via E-prostanoid 1 in Normal Human Dermal Fibroblasts
... Abstract: Collagen type I production decreases with aging, leading to wrinkles and impaired skin function. Prostaglandin E2 (PGE2), a lipid-derived signaling molecule produced from arachidonic acid by cyclo-oxygenase, ...
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Human Cytomegalovirus Glycoprotein B Is Required for Virus Entry and Cell-to-Cell Spread but Not for Virion Attachment, Assembly, or Egress
... pressing normal human dermal fibroblasts (NHDF-gB), which compensated for the loss of the UL55 gene, generating infec- tious virus with a deletion of the UL55 gene in the viral ge- nome but ...
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Constitutive expression of a 92 kD gelatinase (type V collagenase) by rheumatoid synovial fibroblasts and its induction in normal human fibroblasts by inflammatory cytokines
... Synovial fibroblasts freshly isolated from the rheumatoid joint are characterized by their marked connective tissue degradative ...by normal dermal or lung fibroblasts and is immunologically ...
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Effect of MMP-2 gene silencing on radiation-induced DNA damage in human normal dermal fibroblasts and breast cancer cells
... Loss of cell viability of irradiated HDFs and MCF-7 cells is due to DNA damage as detected by immunofluores- cence method using γH2AX antibody. The H2AX is quickly phosphorylated at serine 139 when DSBs arise in the ...
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Heterogeneity in hormone responses and patterns of collagen synthesis in cloned dermal fibroblasts
... Fibroblasts cultured from normal human dermis are heterogeneous with respect to growth kinetics, synthetic function, and morphologic features. There are many examples of clonal heterogeneity in ...
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Polarized subsets of human T helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts
... (typically 200 µ l of cell membranes in 3 ml) and fibroblasts were cultured for four additional hours in 1% FCS medium. When used, intracellular signalling inhibitors (PSI [40 µ mol/l], JNK-inhibitor [10, 20, and ...
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Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1 Induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163 171
... by fibroblasts in normal and pathological states are not completely ...natural human interleukin (IL-1)1 beta and human recombinant (hrIL)-1 alpha and beta are potent stimulators of HA ...
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Elevated miR-34c-5p Mediates Dermal Fibroblast Senescence by Ultraviolet Irradiation
... of normal human primary dermal ...on dermal fibroblasts. MiR-34c-5p expression was increased in dermal fibroblasts after repeated subcytotoxic UVB ...in dermal ...
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Human dermal reticular fibroblasts at confluence display a signature micro pattern in vitro
... of fibroblasts grown from chronic ...C” fibroblasts (their Figure 1 [20]) do show the “phenotype of normal healthy fibroblasts” as the authors have ...
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Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections
... in human dermal fibroblasts (a) and human synovial fibroblasts (b) exposed to Staphylococcus aureus whole cell lysate and filtered cul- ture ...de-identified human dermal ...
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The radiation response of human dermal fibroblasts
... Since the demonstration of a lack of RPLD in fibroblast cultures from AT cells, a number of studies have sought to estabhsh if a greater discrimination is achieved when cells are given an opportunity to undertake RPLD ...
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Evidence for a critical contribution of haploinsufficiency in the complex pathogenesis of Marfan syndrome
... of human fibrillin-1 (C1663R) on a normal mouse ...and human fibrillin-1 interact with high ...a human-specific mAb provides what we believe to be the first demonstration that mutant ...
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Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system
... embedded fibroblasts, where they attached rapidly and formed confluent layers within 1-2 ...of fibroblasts, only thin epithelia developed with rapid loss of proliferation within 2 to 3 days ...of ...
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Activation of endogenous opioid gene expression in human keratinocytes and fibroblasts by pulsed radiofrequency energy fields
... resident dermal and epidermal cells, such as fibroblasts and keratinocytes, as well as infiltrating immune cells) in the proximity of nociceptive nerve termini can modulate nociceptor sensitivity, and thus ...
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Vaccinia Virus Induces Strong Immunoregulatory Cytokine Production in Healthy Human Epidermal Keratinocytes: a Novel Strategy for Immune Evasion
... keratinocytes, dermal fibroblasts, and HMVEC-d were infected with WR-VV at an MOI of 10 (10 PFU per ...and dermal fibroblasts, as viral yield reaches the plateau level (200 to 300 PFU per ...
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Visfatin Promotes Wound Healing Through the Activation of ERK1/2 and JNK1/2 Pathway
... The results of the present study have several critical clinical implications. First, EGF, a typical growth factor, significantly increased the expression of visfatin which was shown to play an important role in wound ...
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Targeting the epigenome: effects of epigenetic treatment strategies on genomic stability in healthy human cells
... for normal breast (MCF-12) and intestinal epithelial cells (FHs 74 int) (Kim et ...vs. normal cells have not been ...ating normal cells with only 4 h for SAHA or TSA and removal 1 h prior to exposure ...
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Effects of Er-Miao-San extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts
... in skin elasticity and degeneration of the extracellular matrix (ECM) [4]. The ECM consists of a mesh of fibrous proteins, such as collagen, elastic fibers, and glycosamino- glycans, which are generated by ...
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Effects of plant sterols derived from <em>Aloe vera</em> gel on human dermal fibroblasts in vitro and on skin condition in Japanese women
... Results: After cocultivation with Aloe sterols, the production of collagen and hyaluronic acid increased by approximately two-fold and 1.5-fold, and gene expression levels of these enzymes responsible for their synthesis ...
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Honey exposure stimulates wound repair of human dermal fibroblasts
... In summary, our present and previous findings consistently indicate that honey is generally active in facilitating wound closure, thus confirming a bulk of anecdotal and scientific evidence. However, the novelty of our ...
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