NSS protein
The Andes Hantavirus NSs Protein Is Expressed from the Viral Small mRNA by a Leaky Scanning Mechanism
... His-ANDV NSs protein in Vero E6 ...ADNV NSs and ANDV N ...His-ANDV NSs protein, was transfected in Vero E6 cells, and 48 h posttransfection total proteins were ...His-ANDV NSs ...
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Rift Valley fever phlebovirus NSs protein core domain structure suggests molecular basis for nuclear filaments
... RVFV NSs (NSs-DNDC) was identified using NMR ...Notably, NSs molecules packed in crystals in double-helical fibrils stabilized by multiple ...nuclear NSs filaments in RVFV-infected ...encoding ...
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NSs Protein of Rift Valley Fever Virus Promotes Posttranslational Downregulation of the TFIIH Subunit p62
... proteins, NSs and NSm, and a less well-characterized 78-kDa protein ...78-kDa protein are both encoded by the M segment (46) and are dispensable for viral replication and infection (11, ...The ...
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Viruses in the Anopheles A, Anopheles B, and Tete Serogroups in the Orthobunyavirus Genus (Family Bunyaviridae) Do Not Encode an NSs Protein
... and NSs (nonstructural), in overlapping open reading frames (ORFs) that are translated from the same ...The NSs proteins of Bunyamwera virus (BUNV) and California serogroup viruses have been shown to play a ...
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Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I
... TOSV NSs protein is a gamma interferon (IFN-  ) antagonist when transiently over- expressed in mammalian cells, inhibiting IRF-3 induction ...RIG-I protein levels in cells overexpressing TOSV ...
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Toscana Virus NSs Protein Promotes Degradation of Double-Stranded RNA-Dependent Protein Kinase
... TOSV NSs protein interacts with PKR in infected cells.TOSV NSs could potentially target PKR directly to the proteasome or promote its degradation through subverting a thus-far-unidenti- fied host ...
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Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response
... RVFV NSs protein is considerably larger that that of ortho- bunyaviruses (31 kDa versus 11 kDa), shows no obvious sequence similarity, and is expressed via an ambisense coding strategy from a specific ...
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The N terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism
... The results presented in Figs 2 and 3 demonstrate the requirement of the first 21 aa of the NSs protein for its IFN-antagonist function. Although the interaction between NSs and Med8 is thought to be ...
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Nonstructural NSs Protein of Rift Valley Fever Virus Interacts with Pericentromeric DNA Sequences of the Host Cell, Inducing Chromosome Cohesion and Segregation Defects
... NSm protein, encoded by RVFV M segment, suppresses virus induced apoptosis (37) and, on the other hand, that NSs protein induces the specific degrada- tion of double-stranded RNA-dependent ...
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The NSs Protein of Tomato spotted wilt virus Is Required for Persistent Infection and Transmission by Frankliniella occidentalis
... of NSs-defective isolates p105RBMar and p202/3RB showed, in both cases, the accumula- tion of viruses in second-instar larvae, while very low accumula- tion was observed in adults, compared with their parental ...
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Rift Valley fever virus NSs protein functions and the similarity to other bunyavirus NSs proteins
... Cellular protein degradation occurs mainly through the ubiquitin (Ub)-proteasome system, where polyubiquitina- tion of proteins with chains of at least four Lys 48-linked Ubs leads to degradation through the 26S ...
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NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3
... phleboviral NSs proteins with respect to sequences, subcellular localizations, and molecular mechanisms, it is tempting to speculate on a correlation between the specific anti-IFN strategy of a given NSs ...
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The Carboxy-Terminal Acidic Domain of Rift Valley Fever Virus NSs Protein Is Essential for the Formation of Filamentous Structures but Not for the Nuclear Localization of the Protein
... why NSs is transported to the nucleus even though all the steps of the viral cycle are localized in the cytoplasmic compartment is still ...of NSs and fila- ment ...virus-induced NSs filaments, these ...
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Large-Scale Chromatin Immunoprecipitation with Promoter Sequence Microarray Analysis of the Interaction of the NSs Protein of Rift Valley Fever Virus with Regulatory DNA Regions of the Host Genome
... nonstructural NSs protein) or the RVFV ZH ⌬ NSs strain (not expressing the NSs ...using protein A agarose-salmon sperm DNA beads from ...
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NSs Protein of Rift Valley Fever Virus Induces the Specific Degradation of the Double-Stranded RNA-Dependent Protein Kinase
... viral protein NSs, which was shown to prevent transcriptional induction of the antivirally active type I interferons (alpha/beta interferon [IFN- ␣ /  ...the NSs gene induce synthesis of IFNs and ...
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Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs
... the NSs protein of BUNV inhibits phosphorylation of the C-terminal domain of RNA polymerase II, thus aborting transcription of host cell mRNAs ...the NSs protein of Rift Valley fever virus ...
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Attenuation of bunyavirus replication by modification of genomic untranslated regions
... the NSs protein across genera and, even within the same genus, sequences are poorly ...The NSs’ of the different genera appear to be multifunctional proteins involved in virus replication and host ...
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Genetic Evidence for an Interferon-Antagonistic Function of Rift Valley Fever Virus Nonstructural Protein NSs
... defective NSs gene (45). The wild-type NSs gene codes for a nonstructural protein of 265 amino acids that is abundantly expressed in infected ...The NSs gene of clone 13 has a large internal ...
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Reverse Genetics System for Uukuniemi Virus (Bunyaviridae): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs
... segment-derived NSs protein has remained elusive. We found that NSs was not required for CAT ...the NSs gene, has shown that it replicates normally in some cell lines while establishing ...
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Tula hantavirus isolate with the full length ORF for nonstructural protein NSs survives for more consequent passages in interferon competent cells than the isolate having truncated NSs ORF
... MRC5 cells were infected with the mixture of TULV/Lodz and TULV/Moravia isolates. The supernatant was col- lected and used to infect fresh cells. Altogether 6 passages were performed and the RNA was analyzed by RT-PCR ...
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