Nuclear poly(a)-binding protein
The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A).
... Our results indicate that the NS1 protein would be expected to inhibit the nuclear export of all polyA-containing cellular and viral mRNAs in infected cells.. Why would this function of [r] ...
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Control of mRNA stability contributes to low levels of nuclear poly(A) binding protein 1 (PABPN1) in skeletal muscle
... this protein is un- known [12,15]. The nuclear aggregates observed in muscle of OPMD patients may exert toxic effects in the tissue as hypothesized for other polyglutamine and polyalanine ex- pansion ...
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Nuclear Localization of Cytoplasmic Poly(A)-Binding Protein upon Rotavirus Infection Involves the Interaction of NSP3 with eIF4G and RoXaN
... its binding to ...eIF4G binding, we first built a model of the three-dimensional structure of the 163-to-239 coiled-coil region of NSP3 and compared it to the three-dimensional structure of the focal ...
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Functional Characterization of Three Leishmania Poly(A) Binding Protein Homologues with Distinct Binding Properties to RNA and Protein Partners
... either protein was observed in the precipitated complexes, with both supernatant and input samples containing equivalent quantities of the two ...each protein, followed by incubation with Alexa ...
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Specific nuclear binding of adenosine 3',5' monophosphate binding protein complex with subsequent poly(A) RNA synthesis in embryonic chick cartilage
... nuclear binding. However, in the presence of cytosol proteins, nuclear binding of [32P]N3cAMP was demonstrable that was specific, time dependent, and dependent on a heat-labile cytosol ...the ...
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Nuclear, Cytosolic, and Surface-Localized Poly(A)-Binding Proteins of Plasmodium yoelii
... of poly(A)-binding ...RNA- binding proteins identified three possible poly(A)-binding proteins (PABP) in Plasmo- dium yoelii, which we here call PyPABP1 (PY17X_1441700), PyPABP2 ...
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Functional analysis of def-3, a novel dynamic nuclear RNA-binding protein
... luciferase protein provided in the TnT kit was ...the binding assay along w ith co n tro l resins; polyacrylhydrazido-agarose, as a control for poly-G and U, and agarose as a control for ...
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RNA-Binding Domain Proteins in Kinetoplastids: a Comparative Analysis
... two poly(A) binding proteins; splicing factors U1 70k, U2AF35, and U2AF65; rRNA-pro- cessing protein MRD1; peptidyl-prolyl cis-trans isomerase; hnRNPH; translation factor SGN1; nuclear cap ...
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Poly(A) Polymerase and the Nuclear Poly(A) Binding Protein, PABPN1, Coordinate the Splicing and Degradation of a Subset of Human Pre-mRNAs
... Many splicing factors are recruited to RNA polymerase II (Pol II) and assemble on introns during transcription (2, 3). Following these cotranscriptional commitment steps, multiple observations concur in showing that ...
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Rotavirus NSP3 Is a Translational Surrogate of the Poly(A) Binding Protein-Poly(A) Complex
... host poly(A) mRNA translation ...mRNA nuclear export rather than translation inhibition accounts for the rotavirus-mediated extinction of host protein synthesis ...of poly(A) mRNA translation ...
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
... through binding with high affinity to its poly (A) tail tract (Wigington et ...of poly (A) tail is varied from organism to another but generally it consists of 90 – 250 adenosine residues (Goss and ...
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Interactions of Viral Protein 3CD and Poly(rC) Binding Protein with the 5′ Untranslated Region of the Poliovirus Genome
... FIG. 2. Effect of mutations within stem-loop IV on PCBP binding and viral translation. (A) Position and sequence of mutations within the predicted secondary structure of stem-loop IV. The C-rich loop a and loop b ...
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Human immunodeficiency virus type 1 Nef protein inhibits NF-kappa B induction in human T cells.
... Expression of the type 1 human immunodeficiency virus nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA.. Binding of a nuclear protein to the cycl[r] ...
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Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer.
... 4808 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified a protein factor, PEBP2 polyomavirus enhancer-binding protein, in the nuclear extract[r] ...
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The Cellular DNA Helicase ChlR1 Regulates Chromatin and Nuclear Matrix Attachment of the Human Papillomavirus 16 E2 Protein and High-Copy-Number Viral Genome Establishment
... complex protein RFC-Ctf18, which is involved in sister chromatid cohesion establishment and replication fork stabilization ...cell nuclear antigen (PCNA) and the flap endonuclease, Fen1 (35, 36), and is ...
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Azithromycin suppresses interleukin-12p40 expression in lipopolysaccharide and interferon-γ stimulated macrophages
... μg nuclear protein. Nuclear proteins were diluted into RIPA buffer containing both prote- ase and phosphatase inhibitors [18], and incubated with 1 μ L of phosphotyrosine antibody (4G10, Milli- pore, ...
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Efficient Cleavage of Ribosome-Associated Poly(A)-Binding Protein by Enterovirus 3C Protease
... the poly(A) tail of mRNA with high affinity primarily via its RRM 1 and RRM 2 domains (13, 44, 52, ...the poly(A) tail (44, ...the poly(A) tail was inhibited by its interaction with eRF3, leading to ...
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Cleavage of Poly(A)-Binding Protein by Enterovirus Proteases Concurrent with Inhibition of Translation In Vitro
... only transiently observed cleavage products detectable by im- munoblotting with this antibody (see Fig. 5), this decrease was most likely due to a proteolytic degradation. The extent of cleavage of PABP varies slightly ...
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The Relationship between R&D Investment and Dividend Payment Tax Incentives and Their Role in the Dividend Tax Puzzle
... Oas1b protein, but the Oas1a protein contains a K to I substitution in this motif and it is possible that this mutation could affect protein-protein interactions facilitated via the CFK ...
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Proteomic Analysis of the Kaposi's Sarcoma-Associated Herpesvirus Terminal Repeat Element Binding Proteins
... interacts with many cellular factors, including RING3, pRb, p53, HP1, CREB binding protein, mSin3, MeCP2, DEK, his- tone H1, ORCs, and glycogen synthase kinase 3  (4, 12, 13, 24, 28, 29, 40, 41, 50, 52). ...
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