Nuclear transfer
Yves Delage (1854 1920) as a forerunner of modern nuclear transfer experiments
... of nuclear transfer, as imagined by Spemann, was not to obtain groups of genetically identical animals, but to test one of Weismann’s hypotheses (1892, ...unequal nuclear division occurring early ...
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Effects of 5 aza 2’ deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryos
... DNA methylation in mammals is an essential process in the regulation of transcription during embryonic de- velopment [8]. However, epigenetic modification of DNA methylation in mammals occurs aberrantly in most cloned ...
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The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer
... embryos. For the HGn30A, HG21C, and HGn8E lines, the embryos were dissected and selected for the GFP+ tissues. For the HGn28A and HGn62A lines, the whole embryos were extracted from the yolk prior to use. Subsequently, ...
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Nuclear remodelling and early development in cryopreserved, porcine primordial germ cells following nuclear transfer into in vitro matured oocytes
... Int J De,' Bini 39 6 19 644 (1995) O,igilla[ A, 'ide Nuclear remodelling and early development in cryopreserved, porcine primordial germ cells following nuclear transfer into in vitro matured oocytes[.] ...
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Generation of bovine transgenics using somatic cell nuclear transfer
... cell nuclear transfer has provided a new way to create transgenic cattle without the drawbacks of past transgenic ...of nuclear reprogramming will ultimately increase the efficiency of NT and ...
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Cloning animals by somatic cell nuclear transfer – biological factors
... of nuclear transfer ...with nuclear transfer have uti- lized a serum starvation ...for nuclear transfer and four calves were born from 28 embryos transferred to 11 recip- ...
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Somatic cell nuclear transfer: failures, successes and the challenges ahead
... Fig. 1. Improvements in somatic cell nuclear transfer (SCNT) techniques. (A) Traditional cloning (TDM). The MII plate is removed from the oocyte by micromanipulation, the enucleated oocyte is directly ...
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HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes
... We have next investigated whether H3.3 deposition on transplanted nuclei is a pre-requirement or a conse- quence of transcriptional reprogramming. We have first asked whether HIRA, known to be required for H3.3 ...
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Faithful reprogramming to pluripotency in mammals what does nuclear transfer teach us?
... post-implantion nuclear transfer ...through nuclear transfer leads to the rather efficient derivation of both naïve (NT- ESCs) and primed (NT-EpiSCs) pluripotent cell lines in ...
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Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer
... Induced pluripotent stem cells (iPSCs) share similar characteristics with ESCs of indefinite in vitro growth, and may therefore serve as a useful tool for the targeted genetic modification of farm animals via ...
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Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer
... Since the birth of the first mammal, a sheep, by nuclear transfer (Willadsen, 1986) numerous studies have been carried out to elucidate the developmental mechanisms in NT embryos. The potential of this ...
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Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish
... of nuclear transferred embryos [12, 22] and leaves doubt of how the dome stage retardation could occur in zebrafish SCNT embryos derived from other ...after nuclear transfer, the dome stage and ...
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Paternal Mitochondrial DNA Transmission During Nonhuman Primate Nuclear Transfer
... Offspring produced by nuclear transfer (NT) have identical nuclear DNA (nDNA). However, mitochon- drial DNA (mtDNA) inheritance could vary considerably. In sheep, homoplasmy is maintained since mtDNA ...
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Nuclear transfer: Progress and quandaries
... and offspring. Most cloned embryos lose their develop- mental abilities during pre-implantation and gastrulation. Moreover, the surviving adults often show abnormalities. To better understand the issues controlling ...
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Recipient cell nuclear factors are required for reprogramming by nuclear transfer
... Nuclear transfer allows the reprogramming of somatic cells to ...essential nuclear factors, such as Brg1, were retained in the recipient cell following ...embryonic nuclear factors along with ...
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Sperm Nuclear Transfer and Transgenic Production in the Fish Medaka
... Sperm nuclear transfer or intracytoplasmic sperm injection (ICSI) is a powerful assisted re- productive technology (ART) for treating human male ...sperm nuclear transplants showed germline ...sperm ...
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Altered Nuclear Transfer Violates Natural Law Ethics
... As the ethical discussion continues, researchers are pursuing alternative methods to generate stem cells that are ethically less controversial. One method proposed by William Hurlbut and expanded on by Maureen Condic is ...
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Creating genetically modified pigs by using nuclear transfer
... cells. Nuclear transfer from non-pluripotent cell lines provides an alternative to the ES cell route for introduction of targeted gene alterations into the germ ...
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Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs
... as nuclear transplantation (nuclear trans- fer) [42, 43], cell fusion [44], and direct reprogram- ming to pluripotency ...After nuclear transfer, a nuclear reprogramming strategy, MEFNT ...
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Arrest of micronuclear DNA replication during genomic exclusion in Tetrahymena produces haploid strains.
... We decided to treat A*V X diploid conjugating pairs with cycloheximide at the time when pronuclei appear in the normal mate and unilateral nuclear transfer occurs..[r] ...
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