nucleic acid extraction
Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens
... a nucleic acid amplification assay after nucleic acid extraction with an automated system is similar to or better than the sensitivity obtained after manual nucleic acid ...
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Real Time PCR for detection of herpes simplex virus without nucleic acid extraction
... of nucleic acid extraction steps in cases where dilution of potential inhibitors takes place during speci- men collection or in cases where the clinically collected specimen is thought to be ...
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Comparative Evaluation of Three Commercial Systems for Nucleic Acid Extraction from Urine Specimens
... of nucleic acid targets, optimal removal of amplification inhibitors, elimina- tion of components which affect other enzymatic substrates, and sterilization of potentially hazardous ...low-throughput ...
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Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNA
... perform extraction, limited throughput, and technician-de- pendent variability in the efficacy of ...extract nucleic acids from clinical specimens may represent an improvement in molecular tests since they ...
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Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections
... gen nucleic acid extraction from the whole blood is sufficiently efficient, the assays could be used for detecting BSI pathogens from a direct blood draw without the need for a primary culture ...
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Diagnosing Herpesvirus Infections by Real Time Amplification and Rapid Culture
... In our laboratory setting, the rapid cell culture inoculation of the monolayers is performed each working day shortly after arrival of the specimens; after 48 h the detection of viral anti- gens is executed, and positive ...
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Extraction of DNA from plant and fungus tissues in situ
... extract nucleic acids in the field are easily transported to, and utilized at, the collection ...DNA extraction was per- formed with three types of microcentrifuges: electric, battery-operated, and ...
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Identification and Differentiation of Clinically Relevant Mycobacterium Species Directly from Acid Fast Bacillus Positive Culture Broth
... Nucleic acid extraction. Total nucleic acids were extracted from the AFB- positive MGIT broth medium specimens by using a NucliSens easyMAG system (bioMerieux, ...default extraction ...
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Real Time Detection of Influenza A, Influenza B, and Respiratory Syncytial Virus A and B in Respiratory Specimens by Use of Nanoparticle Probes
... couple nucleic acid amplification with a microarray-based scheme for ...and extraction steps prior to starting the assay (4, 16), which can add up to 2 h to the total time to result and may ...
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Comparison of Automated Quantitative Reverse Transcription PCR and Direct Fluorescent Antibody Detection for Routine Rabies Diagnosis in the United States
... subsequent nucleic acid extraction, was performed using the Complex200_OBL_V4_DSP protocol and the QIAsymphony DSP virus/pathogen minikit as per the manufacturer’s ins- ...
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High Throughput Detection of West Nile Virus RNA
... quantitate nucleic acid. For nucleic acid extraction, most commercial kits use a silica gel-based membrane to se- lectively bind RNA or DNA under differently formulated so- ...
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Quantitative and Qualitative Chemical Extraction of Deoxyribo Nucleic Acid DNA from Human Cell Organelles
... The DNA was extracted from 10 samples. The DNA quantity and quality were checked in spectrophotometer analysis and 0.8% agarose gel electrophoresis. The size standard marker was run along the run in the gel ...
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The relation between cell wall protein and nucleic acid synthesis in tall pea plants treated with a growth retardant AMO-1618.
... Cherry(l965). Homogenize the 2 gm of P -labeled tissue in a solution containing 40 ml 0.01 M Tris, pH 7.6, 0.06 M KCl, 0.01 M MgClg: 1 ml bentonite (40 mg/ml); 4.6 ml 11% Dupanol and 60 ml phenol with a Sorvall ...
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Whole body composition of Xenopus laevis larvae: implications for lean body mass during development
... larval Ambystoma tigrinum, in which DNA content was linearly correlated with wet mass (A. W. Smits, unpublished data). Although few studies exist which have correlated nucleic acid content with wet or dry ...
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An Introduction to Peptide Nucleic Acid
... The neutral character of the PNA backbone is an important feature that has many consequences. One of the most impressive is the stronger binding between complementary PNA/DNA strands than between complementary DNA/DNA ...
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Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice
... Nucleic acid vaccine is one of the hot spot of PCV2 vaccine ...PCV2 Nucleic acid vaccine was first re- ported by Kamstrup [16], they developed and investigated the potential of DNA vaccination ...
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Nucleic acid chaperons: a theory of an RNA-assisted protein folding
... amino acid replacement, and that if such changes affect protein folding, this would have restrained such nucleotide replacements ...amino acid co-locations is less than 200 (20 × 20/2), and the pos- sible ...
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The concept of RNA-assisted protein folding: the role of tRNA
... When speaking about tRNA the reader has to keep in mind that translation is still a very “hot” subject for many scientists, “the dark side of molecular biology” [14]dri- ven by a powerful paradigm. This 50 year old ...
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Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus
... Nucleic acid detection kits for SFTSV (PCR-fluores- cence probe techniques) were obtained from SinoMD Gene (Beijing, China) and DaAn Gene (Guangzhou, ...RNA extraction QIAamp Viral RNA Mini Kit (cat ...
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Advances in single molecule nucleic acid sequencing
... The sequencing chamber consists of a flexible plastic adhesive flow cell, less than a millimeter in height, which has been applied on the top of a low-autofluorescence microscope coverslip. The sequencing chamber holds ...
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