Nucleoside-triphosphatase
Nonspecific Nucleoside Triphosphatase P4 of Double-Stranded RNA Bacteriophage φ6 Is Required for Single-Stranded RNA Packaging and Transcription
... requiring nucleoside triphosphates. Protein P4, a nonspecific nucleoside triphosphatase, has previously been suggested to be the prime candidate for the viral packaging engine, based on its location ...
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Polynucleotide modulation of the protease, nucleoside triphosphatase, and helicase activities of a hepatitis C virus NS3-NS4A complex isolated from transfected COS cells.
... The hepatitis C virus (HCV) nonstructural 3 protein (NS3) is a 70-kDa multifunctional enzyme with three known catalytic activities segregated in two somewhat independent domains. The essential machinery of a serine ...
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The Serine Protease and RNA-Stimulated Nucleoside Triphosphatase and RNA Helicase Functional Domains of Dengue Virus Type 2 NS3 Converge within a Region of 20 Amino Acids
... NS3 protein of dengue virus type 2 has a serine protease domain within the N-terminal 180 residues. NS2B is required for NS3 to form an active protease involved in processing of the viral polyprotein precursor. The ...
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Mutational Analysis of the Role of Nucleoside Triphosphatase P4 in the Assembly of the RNA Polymerase Complex of Bacteriophage φ6
... potent nucleoside triphosphatase that provides the energy for the RNA translocation ...P4 nucleoside triphosphate binding site destroys the ability to form ...
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Detergent-inhibited, heat-labile nucleoside triphosphatase in cores of avian myeloblastosis virus.
... Detergent-Inhibited, Heat-Labile Nucleoside Triphosphatase in Cores of Avian Myeloblastosis Virus KAJ FRANK JENSENt Department of Biochemistry, College of Agricultural and Life Sciences,[r] ...
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Inhibition of virion-associated reverse transcription by nucleoside triphosphatase in avian myeloblastosis virus.
... It appears that nucleoside triphosphatase associated with virions released by leukemic my- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest crease in RDDP activity demon[r] ...
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Purification and Characterization of West Nile Virus Nucleoside Triphosphatase (NTPase)/Helicase: Evidence for Dissociation of the NTPase and Helicase Activities of the Enzyme
... RNA-stimulated nucleoside triphosphatase (NTPase) localized in the NS3 protein, and RNA-directed RNA poly- merase associated with the NS5 protein (3, 16, ...
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Polypeptide p41 of a Norwalk-Like Virus Is a Nucleic Acid-Independent Nucleoside Triphosphatase
... Picornaviruses have been extensively studied, and among them poliovirus (PV) is one of the best characterized (66). The nonstructural protein 2C contains the motifs A, B, and C related to nucleoside ...
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The vaccinia virus D5 protein, which is required for DNA replication, is a nucleic acid-independent nucleoside triphosphatase.
... D5 is a nucleic acid-independent NTPase. The D5 protein possesses a characteristic extended type A site of the purine nucleotide binding motif found in NTP-hydrolyzing enzymes such as the vaccinia virus thymidine and ...
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Multimers Formed by the Rotavirus Nonstructural Protein NSP2 Bind to RNA and Have Nucleoside Triphosphatase Activity
... The nonstructural protein NSP2 is a component of rotavirus replication intermediates and accumulates in cytoplasmic inclusions (viroplasms), sites of genome RNA replication and the assembly of subviral particles. To ...
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Brome Mosaic Virus 1a Nucleoside Triphosphatase/Helicase Domain Plays Crucial Roles in Recruiting RNA Replication Templates
... tion complex. When preformed RNA replication complexes are shifted to a nonpermissive temperature, a strong temper- ature-sensitive insertion mutation near the 1a NTPase domain blocks further synthesis of positive- and ...
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Double-stranded RNA bacteriophage phi 6 protein P4 is an unspecific nucleoside triphosphatase activated by calcium ions.
... Double-stranded RNA bacteriophage f 6 has an envelope surrounding the nucleocapsid (NC). The NC is composed of a surface protein, P8, and proteins P1, P2, P4, and P7, which form a dodecahedral polymerase complex ...
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Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes.
... The ATPase activity in the presence of the indicated polynucleotides was determined for the ntNS3HCV enzyme Table 1, the BVDV p87 protein 32, and the YFV ntNS3 protein 35 and then divide[r] ...
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The Nucleoside Triphosphatase and Helicase Activities of Vaccinia Virus NPH-II Are Essential for Virus Replication
... Vaccinia virus NPH-II (nucleoside triphosphate [NTP] phosphohydrolase II) is an NTP-dependent helicase that cat- alyzes unidirectional unwinding of 39-tailed duplex RNAs (27). NPH-II is a member of the DExH family ...
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Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein
... We have produced a murine monoclonal antibody (MAb), ZX10, recognizing the NTPase/helicase domain of the hepatitis C virus (HCV) nonstructural 3 protein (NS3), from which we designed a s[r] ...
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RNA 5′-Triphosphatase, Nucleoside Triphosphatase, and Guanylyltransferase Activities of Baculovirus LEF-4 Protein
... RNA triphosphatase (51), we have the capacity to screen by eye for conservation of essential side ...RNA triphosphatase Cet1p, in the triphosphatase-guanylyltransferase domains of the vaccinia virus, ...
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Purification and properties of a catalytically active fragment of soluble nucleoside triphosphatase from bovine kidney
... Для выделения субъединицы NTPазы 500 г почек быка гомогенизировали в 1,25 л 20 мМ трисНСlбуфера (рН 7,5), содержаще го 0,15 М КСl, 1 мМ ЭДТА и 5 мМ DТТ, в размельчителе тканей РТ1[r] ...
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Core protein mu2 is a second determinant of nucleoside triphosphatase activities by reovirus cores.
... T3D cores at a lower temperature (35°C) and higher pH (pH 8.5). Its results implicated the L3 gene in determining this difference and thus suggested that ATPase activity is strongly influenced by the l 1 protein under ...
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Structure of the Dengue Virus Helicase/Nucleoside Triphosphatase Catalytic Domain at a Resolution of 2.4 Å
... Dengue fever is an important emerging public health concern, with several million viral infections occurring annually, for which no effective therapy currently exists. The NS3 protein from Dengue virus is a multifunc- ...
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Functional Characterisation of the Flax Rust AvrP/AvrP123 Avirulence Proteins
... minute s mildew A Murashige-Skoog nucleotide-binding leucine-rich repeat nucleotide nucleoside-triphosphatase pathogen-associated molecular pattern plant cell death Phytophthora cactorum[r] ...
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