Nucleotidase activity
Alpha 1 adrenoceptor activation mediates the infarct size limiting effect of ischemic preconditioning through augmentation of 5' nucleotidase activity
... We have reported that ischemic preconditioning may limit infarct size by increasing 5'- nucleotidase activity. The present study tested whether alpha 1-adrenoceptor stimulation in ischemic preconditioning ...
10
Heterogeneity of 5′ Nucleotidase Activity in Lymphocytes in Chronic Lymphocytic Leukemia
... specific activity of 5¢-nucleotidase was determined in lymphocyte plasma membranes from 14 normal subjects and 10 patients with chronic lymphocytic leukemia ...5¢-nucleotidase activity. The ...
5
Ecto 5′ Nucleotidase Activity in Human T Cell Subsets DECREASED NUMBERS OF ECTO 5′ NUCLEOTIDASE POSITIVE CELLS FROM BOTH OKT4+ AND OKT8+ CELLS IN PATIENTS WITH HYPOGAMMAGLOBULINEMIA
... (cytotoxic/suppressor) cells. Total T cell ecto-5¢-nucleotidase activity was 10.9±2.1 nmol/h per 10 6 cells with 25±7% positive by histochemical stain. Ecto-5¢-nucleotidase activity in ...
9
Early events after infection of Escherichia coli by bacteriophage T5. Induction of a 5'-nucleotidase activity and excretion of free bases.
... Since the specific activity of dTMP nucleotidase in infected cells was only 60 nmol per min per mg at its maximal level, the host thymidine phosphorylase activity was about twice that of[r] ...
8
Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.
... The presence of an actual protein-caused inactivation in Al-infected cells has been further substantiated by noting that when chloramphenicol is added to Al-infected cells before 6 min a[r] ...
9
Bacterial UDP Glucose Hydrolases and P2 Receptor Mediated Responses to Infection: A Commentary
... In Salmonella enterica and Escherichia coli, UDG- glucose (UDPG) hydrolases were discovered as enzymes which catalyse the hydrolysis of the pyrophosphate bond of UDPG leading to the formation of UMP and glu- ...
5
Evidence for deactivation of both ectosolic and cytosolic 5' nucleotidase by adenosine A1 receptor activation in the rat cardiomyocytes
... cyclohexyladenosine, an adenosine A1 receptor agonist. Neither CGS21680C, and A2 receptor agonist, nor cycloheximide deactivated ectosolic and cytosolic 5'-nucleotidase. Thus, we conclude that activation of ...
7
Lead poisoning: association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5' nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines
... cytidine. Nucleotidase activity was about 25% that in normal erythrocytes and 15% or less of that expected in comparable reticulocyte-rich ...of nucleotidase activity in patient erythrocytes ...
8
Morphine treatment alters nucleotidase activities in rat blood serum
... -nucleotidase activity was observed, making it difficult to infer whether this outcome will result in decreased extra- cellular adenosine in ...decreased activity of NTPDase 1 may prolong the effect ...
7
Role of N-Terminal Hydrophobic Region in Modulating the Subcellular Localization and Enzyme Activity of the Bisphosphate Nucleotidase from Debaryomyces hansenii
... enzymatic activity of ATG mutants of ...PAP nucleotidase activity of each fraction is expressed as nmol of ...of activity of each fraction against PAP and ...The activity of each form ...
10
Changes in Adenosine Metabolism in Asthma A Study on Adenosine, 5' NT, Adenosine Deaminase and Its Isoenzyme Levels in Serum, Lymphocytes and Erythrocytes
... 5ʹ-nucleotidase activity in serum and lym- phocytes was raised in moderate and severe persistent groups and an inverse correlation existed between 5ʹ-nucleotidase activity and FEV1% whereas in ...
17
Elevated 5' nucleotidase and alkaline phosphodiesterase activities in trout gill localize to endothelial (pillar) cells
... 5′NT activity in trout tissues was concentrated in membrane fractions purified by density gradient centrifugation, and these fractions were also specifically enriched with a variety of other plasma membrane ...
9
Regulatory processes in the central nervous system: A study of g-protein abundance and of soluble 5'-nucleotidase
... increased activity in the hypothyroid ...soluble activity, it was decided to embark on such a ...cytosolic activity failed to show activation by ...soluble activity appeared to be inhibited, a ...
235
A proteomics approach reveals molecular manipulators of distinct cellular processes in the salivary glands of Glossina m morsitans in response to Trypanosoma b brucei infections
... 5 ′ Nuc, 5 ′ -nucleotidase-related saliva protein; ABC, ABC ammonium bicarbonate; ACN, acetonitrile; ADA, salivary adenosine deaminase; ADGF, adenosine deaminase growth factor; AhpC, alkyl hydroperoxide reductase; ...
19
Impairment of tubuloglomerular feedback regulation of GFR in ecto 5′ nucleotidase/CD73–deficient mice
... Nevertheless, these two lines of experimental evidence can be reconciled by assuming that ATP is successively hydrolyzed to 5′-AMP and adenosine in the juxtaglomerular interstitium so that ATP released by MD cells serves ...
10
Comparison of the efficacy and safety of FP 1201 lyo (intravenously administered recombinant human interferon beta 1a) and placebo in the treatment of patients with moderate or severe acute respiratory distress syndrome: study protocol for a randomized controlled trial
... Background: Acute respiratory distress syndrome (ARDS) results in vascular leakage, inflammation and respiratory failure. There are currently no approved pharmacological treatments for ARDS and standard of care involves ...
9
SERUM INHIBITION OF STREPTOCOCCAL DIPHOSPHOPYRIDINE NUCLEOTIDASE IN UNCOMPLICATED STREPTOCOCCAL PHARYNGITIS AND IN RHEUMATIC FEVER
... The streptococcal antibody response was analyzed in three ways: a the geometric mean of the titers of all patients' acute phase sera was compared with that of all patients' convalescent [r] ...
9
Oligomeric interface modulation causes misregulation of purine 5´-nucleotidase in relapsed leukemia
... and motif III (K292, D351, and D359) [8]. To achieve a full catalytic activity, cN-II requires the presence of adenylate compounds, such as ATP, adenosine diphosphate or diade- nosine tetraphosphate [1]. Binding ...
16
Tissue Guanosine 3′,5′ Cyclic Monophosphate Levels and Soluble Guanylate Cyclase Activity: A POSITIVE CORRELATION DURING UNILATERAL CRYPTORCHIDISM IN THE RAT TESTIS
... cyclase activity was only slightly elevated 10 and 20 days postoperatively, a 200% increase in the soluble guanylate cyclase activity was seen at 5 ...in activity was >250% (P < ...cyclase ...
10
Acidosis during ischemia promotes adenosine triphosphate resynthesis in postischemic rat heart In vivo regulation of 5' nucleotidase
... Capacity for ATP resynthesis during recovery from ischemia or hypoxia is limited to the size of the adenine nucleotide pool, which is determined in part by the activity of cytosolic 5'- nucleotidase ...
11