OGT, O-GlcNAc transferase
Original Article Increased expression of O-GlcNAc transferase (OGT) is a biomarker for poor prognosis and allows tumorigenesis and invasion in colon cancer
... Elevated O-GlcNAcylation by increased O-GlcNAc transferase (OGT) and/or decreasing O-GlcNAcase (OGA) levels is associated with cancer initiation, progression, invasion, and ...of ...
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<p>Suppressed OGT expression inhibits cell proliferation and modulates EGFR expression in renal cell carcinoma</p>
... of O-glcnacylation and OgT in RCC (786-O, aChn, CaKi-1, and a498) cell lines compared with the human normal renal tubular epithelial hK-2 cell ...of OgT in RCC and adjacent normal tissues were ...
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Metabolic inhibitors of O GlcNAc transferase (OGT) that act in vivo implicate decreased O GlcNAc levels in leptin mediated nutrient sensing
... Abstract: O-Linked glycosylation of serine and threonine residues of nucleocytoplasmic proteins with N-acetylglucos- amine (O-GlcNAc) residues is catalyzed by O-GlcNAc trans- ferase ...
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Identification of Secret Agent as the O-GlcNAc Transferase That Participates in Plum Pox Virus Infection
... that O-GlcNAc modification by SEC OGT is not essential for PPV viability in ...tion. O-GlcNAc modifications at the segment from amino ac- ids 1 through 39 of PPV CP are not essential, ...
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Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism
... novel OGT inhibitor, OSMI-1, was recently reported with higher specificity against OGT than other extant compounds [18, ...purified OGT, OSMI-1 has a 20-fold lower IC 50 -value once compared to ...
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O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease
... overall O-GlcNAcylated protein levels (p = ...the O-GlcNAc moiety to proteins, O-GlcNAc transferase ...of OGT de- crease in the presence of thiamet G, likely due to a ...
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O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis
... and O-GlcNAcylation (Supplemental ...of O-GlcNAcy- lation in regulating GA signaling came from the identi- fication of a putative O-linked N-acetylglucosamine (O-GlcNAc) ...
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Advances in understanding glycosyltransferases from a structural perspective
... An O-linked N-acetylglucosamine residue (O-GlcNAc) is added to serine or threonine residues (via a b-linkage) in a variety of nucleocytoplasmic proteins in ...the O-GlcNAc modification ...
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O-GlcNAc modification is associated with insulin sensitivity in the whole blood of healthy young adult males
... protein O-GlcNAc was examined. O-GlcNAc and O-GlcNAc transferase (OGT) levels were quantified by immunoblotting and compared to anthropometric measures: body mass ...
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O-GlcNAc transferase suppresses necroptosis and liver fibrosis
... in OGT-deficient hepatocytes (Figure ...in OGT siRNA–transfected hepatocytes, indicating that elevated RIPK3 and MLKL expression underlies the vulnerability to necroptotic treatments upon acute OGT ...
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Increased sugar uptake promotes oncogenesis via EPAC/RAP1 and O GlcNAc pathways
... and O-linked N-acetylglucosamine (O-GlcNAc) ...inhibits O-GlcNAc transferase–mediated (OGT-mediated) O-GlcNAcylation (39), also induced phenotypic reversion, ...
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Elevated O-GlcNAcylation promotes gastric cancer cells proliferation by modulating cell cycle related proteins and ERK 1/2 signaling
... O-GlcNAc transferase (OGT) is the only enzyme in mammals that catalyzes the attachment of β-D-N-acetylglucosamine (GlcNAc) to serine or threonine residues of target ...that ...
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Down-regulation of OGT promotes cisplatin resistance by inducing autophagy in ovarian cancer
... that OGT knockdown induces autolysosome formation, which promotes autophagic ...is O-GlcNAcylated and the O-GlcNAcylation of SNAP-29 regulates the formation of a complex comprising SNAP-29, ...
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Investigating the Role of O-GlcNAc Glycosylation in Neurodegeneration
... Radioactive asasys were run essentially as described previously. Briefly, cells were lysed in 20mM Tris 7.5, 10mM MgCl2, 1mM EDTA, PMSF/PIC, 1mM Na2VO4, 10mM NaF, in the presence or absense of 1% NP-40. Lysates were spun ...
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A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
... of OGT, several methods have been put forward based on detection of glycosylated prod- ucts, including (1) FRET method (3), (2) MS-based method (4, 5) and (3) radioactive label methods (6, ...
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Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice
... of O-tau in HEK293 cell lysates and in mouse brain homogenates using antibody ...of O-tau in HEK293 ...Purified O-tau from Sf9 cells was also loaded as a ...detected O-tau in HEK293 cells only ...
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O-GlcNAc modification of Sox2 regulates self-renewal in pancreatic cancer by promoting its stability
... on O-GlcNAc modification of ...an O-GlcNAc ...when OGT and Sox2 were overexpressed in OGTi cells, a robust increase in colony formation was seen, indicating a full rescue ...
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DYSREGULATION OF O-LINKED β-N-ACETYLGLUCOSAMINE (O-GLCNAC) CYCLING SUPPORTS TUMORIGENICITY OF CANCERS OF THE FEMALE REPRODUCTIVE TRACT, Nicole Jaskiewicz
... (Figure 4), despite the observation that K18 and β-actin expression were both diminished. Others have observed that α -tubulin and β -actin expression in colon cancer cells are unaffected by inhibition of OGT, yet ...
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Fueling the fire: emerging role of the hexosamine biosynthetic pathway in cancer
... linking O-GlcNAc/OGT to regu- lation of CSCs is beginning to ...high O-GlcNAc conditions as contributing to tumor progression and self- renewal ...with GlcNAc in glucose- ...
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Glycomic Approaches to Study GlcNAcylation: Protein Identification, Site-mapping, and Site-specific O-GlcNAc Quantitation
... A potential alternative to circumvent enrichment is to apply top-down proteomics [41]. Intact proteins or briefly digested long peptides are highly charged. Thus, the negative effect of a single O-GlcNAc ...
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