Oligodeoxynucleotides, ODN
Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus
... Purified B cells, gating on memory CD19+/CD27+ (Immunotech, Beckman-Coulter, Marsellie, France) B cells, from SLE patients were analyzed for TLR-9 expression. Condition-media from HEK293- cells, which were infected by ...
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Short hairpin looped oligodeoxynucleotides reduce hepatitis C virus replication
... The ODN sequences are shown in Figure 1A, and their target regions on the HCV 5’ NTR, which forms extensive secondary structures, in Figure ...identical ODN without any sequence similarity to the HCV genome ...
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Antibody carriers of CpG oligodeoxynucleotides for solid tumor immunotherapy
... To date, CpG ODNs used in monotherapy for tumor control suffer from lack of an effective route of administration. Although intra- and peri-tumoral injections have been effective in murine models, 1 these routes limit the ...
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Structure-dependent immunostimulatory effect of CpG oligodeoxynucleotides and their delivery system
... Figure 4 Formation of higher-order structure in class A oligodeoxynucleotides (ODNs). (A) Globular structure of class A cytosine-phosphate-guanosine (CpG) ODN observed by atomic force microscopy (large ...
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Toll-like receptor 9 interaction with CpG ODN – An in silico analysis approach
... TLR9 is a receptor for sensing bacterial DNA/CpG-containing oligodeoxynucleotides (CpG ODN) within the endosomal compartment [4]. Internalised CpG DNA within the endosome initiates TLR9-mediated signalling ...
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Immunomodulatory Effects of CpG Oligodeoxynucleotides on Established Th2 Responses
... CpG ODN therapy of allergic asthma, the treatment has been ini- tiated at the systemic sensitization period (24) or before anti- gen challenge (8, 39, ...CpG ODN-induced IFN-␥ and IL-12 production from ...
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Binding, uptake, and intracellular trafficking of phosphorothioate modified oligodeoxynucleotides
... phosphorothioate modified oligodeoxynucleotides (P-ODN) might facilitate the use of these compounds for experimental and therapeutic purposes. We addressed these issues by identifying cell surface proteins ...
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Pleiotropic action of CpG-ODN on endothelium and macrophages attenuates angiogenesis through distinct pathways
... cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN), to investigate the role of TLR9 in vascular pathophysiology and identify potential therapeutic ...
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Short hairpin loop structured oligodeoxynucleotides reduce HSV 1 replication
... each ODN is indicated below the ...of ODN 48 G and S2 as indicated in the presence of transfection reagent (indicated by circle) at the indicated concentrations and were incu- bated over night (o/n) at ...
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<p><em>N</em>-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264.7 Cell Osteoclastogenesis</p>
... CpG ODN 2006, β -actin, Nfatc, c-fos, RANK, matrix metalloproteinase 9 (MMP9), and 6-carboxy fl uores- cein (FAM)-labeled ODN (ODN and phosphorothioate ODN) were synthesized by TaKaRa Bio ...
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Paeoniflorin inhibits excitatory amino acid agonist and high dose morphine induced nociceptive behavior in mice via modulation of N methyl D aspartate receptors
... NMDA receptors [52]. The antinociception effects of PF on amino acid agonists and high-dose morphine- induced nociceptive behavior are mediated by target- ing iGluRs, especially modulation of the NMDA 2B re- ceptor. The ...
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Limited Effect of CpG ODN in Preventing Type 1 Diabetes in NOD Mice
... CpG ODN, once assumed to be a significant suppressor of type 1 diabetes, were no better than any other CpG ...CpG ODN had no protective effects against inflammation of pancreatic islet ...CpG ODN ...
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Expression of c-myc is not critical for cell proliferation in established human leukemia lines
... antisense oligodeoxynucleotides, the unusual biologi- cal activity of these small oligodeoxynucleotides presents the opportunity to define novel targets for antileukemic drug ...
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Antisense technology reveals the alpha2A adrenoceptor to be the subtype mediating the hypnotic response to the highly selective agonist, dexmedetomidine, in the locus coeruleus of the rat
... Alpha2 adrenergic agonists are used in the anesthetic management of the surgical patient for their sedative/hypnotic properties although the alpha2 adrenoceptor subtype responsible for these anesthetic effects is not ...
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CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa
... CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa Tao Qin, Yinyan Yin, Qinghua Yu, [r] ...
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Carbon nanotubes as cancer therapeutic carriers and mediators
... antisense oligodeoxynucleotides; BSA, bovine serum albumin; CDDP, cis-dichlorodiammineplatinum; CNTs, carbon nanotubes; c-MwCNTs, carboxylated MwCNTs; CP, cis-diammine(1,1-cyclobutanedicarboxylato) platinum(II); ...
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Duplex DNA Capture
... an ODN probe, which is mostly determined by salt concentration, ODN length and ODN base ...the ODN tag needs to be rebound to facilitate the subsequent round of OPAC ...
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Investigation of the cytokine response to NF κB decoy oligonucleotide coated polysaccharide based nanoparticles in rheumatoid arthritis in vitro models
... A major barrier in the advancement of nucleic acid ther- apies to achieving clinical relevance is a general lack in ability of the negatively charged nucleic acid to enter the negatively charged cell membrane. A number ...
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Toll like receptor 9–induced type I IFN protects mice from experimental colitis
... mice was not. Here we utilized those ISS-responsive and ISS- resistant phenotypes to better understand the antiinflammatory role of TLR9 signaling in colonic inflammation. Analysis of the response to ISS-ODN of ...
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Phenotypic and functional abnormalities of bone marrow derived dendritic cells in systemic lupus erythematosus
... motifs(CpG ODN)2006 and 2 μmol/L CpG ODN 2216 (InvivoGen, San Diego, CA, USA), 50 ng/ml tumor necrosis factor (TNF)-α (PharMingen), and 25 ng/ml lipopolysaccharide ...
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