oocyst wall
Oocyst wall formation and composition in coccidian parasites
... of oocyst wall formation and structure helps to explain the incredible environmental resilience of coccidian oocysts - quinone tanning and dityrosine crosslinking, leading to sclerotization, are widespread ...
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Evidence for Mucin-Like Glycoproteins That Tether Sporozoites of Cryptosporidium parvum to the Inner Surface of the Oocyst Wall
... purified oocyst walls and of total oocyst proteins puri- fied on MPA affinity ...centrifugation. Oocyst walls were prepared from excysted oo- cysts or sonicated oocysts by being centrifuged twice ...
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Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation
... Disease control relies exclusively on the protective immunity conferred to chickens. Therefore, to immunize chickens against E. necatrix, a planned immunization program with field isolates has been extensively imple- ...
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Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina
... the oocyst wall matrix, and at the morphological level, where a clear, ordered se- quence of changes in structure and location of WFBs can be mapped (using the antibodies to the WFB proteins) as the macro- ...
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Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton Cryptosporidium parvum Oocyst Wall Protein
... species-specific oocyst wall protein of Cryptosporidium ...of oocyst proteins from several different Cryptosporidium ...parvum oocyst surface and was associated with amorphous oocyst ...
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The Glycosylation Pathway of Eimeria tenella Is Upregulated during Gametocyte Development and May Play a Role in Oocyst Wall Formation
... the oocyst wall, a structure that ensures the efficient transmission of these and related ...unsporulated oocyst stages, and accordingly, a significant increase in GalNAc levels was observed in ...
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Roles of Tyrosine-Rich Precursor Glycoproteins and Dityrosine- and 3,4-Dihydroxyphenylalanine-Mediated Protein Cross-Linking in Development of the Oocyst Wall in the Coccidian Parasite Eimeria maxima
... the oocyst wall in Eimeria have not been reported, antibodies to gametocyte glycoproteins have been shown to recognize the oocyst wall in ...the oocyst wall contains antigenic ...
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Identification of enzymes potentially involved in the formation of the oocyst wall of coccidian parasites
... Eimeria oocyst wall at levels that suggest that the formation is an enzyme- mediated reaction (Belli et ...other wall proteins during oocyst wall formation of the parasite ...
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Plasmodium berghei Cap93, a novel oocyst capsule associated protein, plays a role in sporozoite development
... The oocyst capsule is in contact with the basal lamina of the mosquito midgut ...The oocyst capsule helps to fend off defensive responses by the ...known oocyst wall surface protein, PbCap380, ...
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Strategies To Discover the Structural Components of Cyst and Oocyst Walls
... and oocyst walls of Cryptosporidium and ...cyst wall. (B) Antibodies (green) show CWP1 in a shard of the cyst wall of Giardia broken by ...inCryptosporidium oocyst walls. (D) Dityrosines ...
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RNA Seq analysis of the Eimeria tenella gametocyte transcriptome reveals clues about the molecular basis for sexual reproduction and oocyst biogenesis
... Highly abundant upregulated gametocyte transcripts Within the subset of 863 upregulated gametocyte transcripts identified in E. tenella, the level of transcript abundance for each gene in gametocytes, expressed as FPKM ...
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Deposition and adhesion of Cryptosporidium oocysts on surfaces
... Cryptosporidium oocysts in the UK has been well reviewed (DoE- SCA, 1990) and involves the filtration of large volumes of water, depending on turbidity, through a cartridge filter with 1 |im pore size. The oocysts are ...
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Two COWP like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation
... found. COWPs contain motifs (type I and type II) with cysteine residues in conserved and regularly spaced posi- tions [5]. The cysteine residues, so assumed, form disulfide bridges among each other and are responsible ...
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Evaluation of Recombinant Oocyst Protein CP41 for Detection of Cryptosporidium-Specific Antibodies
... Serological assays for anti-Cryptosporidium antibodies pro- vide an important and widely used indicator of prior exposure to the parasite. Earlier studies have established that specific serum IgG can be detected with ...
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Field application of a subunit vaccine against an enteric protozoan disease
... the oocyst wall of the parasite, a structure that is absolutely essential for the survival of the parasites outside of their host and, therefore, vital for transmission of parasites from host to host ...of ...
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Molecular Epidemiological Analysis of Cryptosporidiumspp in the United Kingdom: Results of GenotypingCryptosporidium spp in 1,705 Fecal Samples from Humans and 105 Fecal Samples from Livestock Animals
... Samples were analyzed here from 1 contact group within a nursery and 26 family groups. Among the sporadic cases, these are most likely to represent small intrafamily outbreaks where person-to-person secondary spread has ...
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Genomic Variation in IbA10G2 and Other Patient Derived Cryptosporidium hominis Subtypes
... Cryptosporidium oocyst wall protein 9 (COWP9), which presented a pre- dicted loss-of-function mutation in all the sequence subtypes, except for that seen with IbA10G2, which has a sequence identical to the ...
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Flow cytometric detection of Cryptosporidium oocysts in human stool samples
... Samples were evaluated by using a sampling interval of 102 s. The acquisition gate was set based on the fluorescence and forward scatter characteristics of a sample containing pure oocysts in PBS incubated with ...
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Transmission blocking activity induced by malaria vaccine candidates Pfs25/Pvs25 is a direct and predictable function of antibody titer
... killed is fixed by the feeding conditions and is independ- ent of the oocyst distribution. The fit between the simu- lated and actual data sets for P. vivax is important since the input data on which this ...
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Experimental study on Plasmodium berghei-Anopheles stephensi-BALB/c mouse system; implications for malaria transmission blocking assays
... Feeding the mosquitoes on the infectious mice with high parasitemia or exflagellation centers in each microscopic field result in high oocyst formation, however, the num[r] ...
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