Opsonophagocytic activity
Prediction of Pneumococcal Conjugate Vaccine Effectiveness against Invasive Pneumococcal Disease Using Opsonophagocytic Activity and Antibody Concentrations Determined by Enzyme-Linked Immunosorbent Assay with 22F Adsorption
... and opsonophagocytic activity (OPA) titers, to predict the clinical effectiveness of the 7-valent pneumo- coccal conjugate vaccine (7vCRM) against invasive pneumococcal disease ...
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Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity
... ous infection or recent vaccination and are associated with protection against pertussis. However, the antibody specificity and antibody effector mechanisms for protection remain to be established. Phagocytosis may be an ...
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Opsonophagocytic Activity and Other Serological Indications of Bordetella pertussis Infection in Military Recruits in Norway
... Bordetella pertussis is the causative agent of pertussis (whooping cough). Despite high vaccination coverage, pertussis remains a significant disease in many countries. Besides vaccination, transient carriage of ...
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Antibodies Specific for the Hia Adhesion Proteins of Nontypeable Haemophilus influenzae Mediate Opsonophagocytic Activity
... the opsonophagocytic activity of anti-Hia antibodies against homologous and heterologous ...of opsonophagocytic antibodies and that shared epitopes recognized by such antibodies are present on the ...
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Poor Correlation between Pneumococcal IgG and IgM Titers and Opsonophagocytic Activity in Vaccinated Patients with Multiple Myeloma and Waldenstrom's Macroglobulinemia
... Age is an important factor in evaluations of pneumococcal vaccine responses. Previous studies have shown poorer functional antibody responses after vaccination with PPV among elderly adults than among younger adults (20, ...
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Seroprevalence of Antibody-Mediated, Complement-Dependent Opsonophagocytic Activity against Neisseria meningitidis Serogroup B in England
... OP activity seen in 30- to 39-year- olds is not also seen for bactericidal ...OP activity. Broader cross-strain OP activity than serum bactericidal activity was seen following the immunization ...
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Are the Opsonophagocytic Activities of Antibodies in Infant Sera Measured by Different Pneumococcal Phagocytosis Assays Comparable?
... Host protection against Streptococcus pneumoniae is mainly mediated by opsonin-dependent phagocytosis. Several techniques for measuring opsonophagocytic activity (OPA) of antibodies to S. pneumoniae have ...
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Specificities and Opsonophagocytic Activities of Antibodies to Pneumococcal Capsular Polysaccharides in Sera of Unimmunized Young Children
... An enzyme immunoassay (EIA) for antibodies to pneumococcal capsular polysaccharides (Pnc PSs) detects in some cases antibodies that are cross-reactive within different Pnc PSs. Recently, it has been suggested that for ...
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Multilaboratory Evaluation of a Viability Assay for Measurement of Opsonophagocytic Antibodies Specific to the Capsular Polysaccharides of Streptococcus pneumoniae
... protocol described by Quartaert et al. (20) allowed for the measurement of Ps-specific antibodies in children and adults (6, 19, 18). Adults can have cross-reactive antibodies, which confound the measurements of ...
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Antibodies to the HMW1/HMW2 and Hia Adhesins of Nontypeable Haemophilus influenzae Mediate Broad-Based Opsonophagocytic Killing of Homologous and Heterologous Strains
... Opsonophagocytic activity mediated by anti-HMW1/ HMW2 immune sera with HMW1/HMW2-expressing ...the opsonophagocytic as- say (Table ...to opsonophagocytic killing mediated by preim- mune and ...
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A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
... functional opsonophagocytic activity could be competitively inhibited by the presence of homologous capsular polysac- charide (20); however, we did not address the possible par- ticipation of cross-reactive ...
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Antibodies Specific for the High-Molecular-Weight Adhesion Proteins of Nontypeable Haemophilus influenzae Are Opsonophagocytic for both Homologous and Heterologous Strains
... to opsonophagocytic killing (Table 2), a result similar to those reported previously in our studies of human antibodies di- rected against the HMW1/HMW2-like proteins ...to opsonophagocytic killing by each ...
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Naturally Acquired HMW1- and HMW2-Specific Serum Antibodies in Adults and Children Mediate Opsonophagocytic Killing of Nontypeable Haemophilus influenzae
... Opsonophagocytic activity of HMW1- and HMW2-specific antibodies present in serum samples from four ...functional opsonophagocytic activity of the convalescent- phase serum samples from the ...
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Immunogenicity and safety of a tetanus diphtheria vaccine and a 13 valent pneumococcal conjugate vaccine after concomitant vaccination in ≥ 50 year old adults
... Multiplex opsonophagocytic killing assay; OMP: Complex outer- membrane protein mixture from ...OPA: Opsonophagocytic activity; PCV13: 13-valent pneumococcal conjugate vaccine; Td: Tetanus- ...
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Approach to Validating an Opsonophagocytic Assay for Streptococcus pneumoniae
... PnPs-specific opsonophagocytic activity or nonspecific killing associated with OPA can be mediated by controllable conditions, such as the age ...nophagocytic activity in the presence of abundant ...
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Use of Opsonophagocytosis for Serological Evaluation of Pneumococcal Vaccines
... an opsonophagocytic killing assay and the IgG antibodies as measured by enzyme-linked immunosorbent as- say (ELISA) (12), although the correlation may be limited in some populations, as described later in this ...
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Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera
... ABSTRACT Opsonophagocytic assays are used to measure functional antibodies im- portant in protection against pneumococcal capsular ...from opsonophagocytic assays across the three laboratories may be ...
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Assignment of Opsonic Values to Pneumococcal Reference Serum 007sp for Use in Opsonophagocytic Assays for 13 Serotypes
... In vivo, antibodies against pneumococcal capsular PSs are thought to function by opsonizing the bacteria for subsequent phagocytosis and killing by granulocytes (6). Thus, in vitro opsonophagocytic assays (OPAs) ...
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Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against Streptococcus pyogenes
... Group A streptococci use a number of different mechanisms to resist opsonization and phagocytosis. As a result, they represent a somewhat heterogenous population of bacteria relative to expression of different surface ...
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Multilaboratory Comparison of Streptococcus pneumoniae Opsonophagocytic Killing Assays and Their Level of Agreement for the Determination of Functional Antibody Activity in Human Reference Sera
... tory using cultured phagocytic cell lines and a standardized source of complement (baby rabbit complement) in an opsonophagocytosis assay (OPA) (8, 21). A standardized OPA is needed to consistently evaluate functional ...
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