ORF2 protein
Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein
... untagged ORF2 protein expressed from the plasmids containing full- length wild-type or codon-optimized L1 ...L1 protein function or subcellular localization ...endonuclease protein, we ...
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Torque Teno Virus (SANBAN Isolate) ORF2 Protein Suppresses NF-κB Pathways via Interaction with IκB Kinases
... TTV ORF2 protein can suppress NF- B activity in vivo, we wished to detect whether TTV ORF2 protein can affect these ...TTV ORF2 protein does affect the activity of the IL-6, ...
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Cytoplasmic Localization of the ORF2 Protein of Hepatitis E Virus Is Dependent on Its Ability To Undergo Retrotranslocation from the Endoplasmic Reticulum
... viral protein exploiting the cellular machinery to its ...which ORF2 protein localizes to the cytoplasm despite being an N-linked ...The ORF2 protein has been shown to be present ...
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Expression of TTV ORF2 Protein for Detection of Anti TTV IgG Antibodies in Human Sera
... the protein at 8, 12, 16 and 20 hour post incu- bation at 37˚C (Figure ...of protein was obtained after 16 hr incubation at ...truncated protein of mo- lecular mass ~20 KDa. The protein was ...
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The endonuclease domain of the LINE-1 ORF2 protein can tolerate multiple mutations
... the ORF2 endonuclease ...and ORF2 sequences (no gaps, premature stops or ...for ORF2 endonuclease function [8, ...the ORF2 protein sequences of the full-length L1 loci ex- tracted from ...
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Mutational Analysis of Glycosylation, Membrane Translocation, and Cell Surface Expression of the Hepatitis E Virus ORF2 Protein
... Brefeldin A is a fungal metabolite that disrupts intracellular membrane traffic at the ER-Golgi junction (20), resulting in a redistribution of Golgi enzymes into the ER. Since BfA col- lapses Golgi enzymes into the ER, ...
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Hepatitis E virus ORF2 protein over expressed by baculovirus in hepatoma cells, efficiently encapsidates and transmits the viral RNA to naïve cells
... the ORF2 protein detected by Western blot was 72 kDa, the size expected for the full- length ...exogenously-supplied ORF2 protein could transencapsidate viral genomes, S10- 3 cell cultures ...
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Immunization of mice by Hollow Mesoporous Silica Nanoparticles as carriers of Porcine Circovirus Type 2 ORF2 Protein
... PCV2 ORF2 protein, the antigenitic epitope at amino acid resi- dues 113–147 of PCV2 ORF2 [21] was expressed; this epitope was found to be the immunorelevant epitope for virus type ...
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Transgenic Plants Expressing Potato Virus X ORF2 Protein (p24) Are Resistant to Tobacco Mosaic Virus and Ob Tobamoviruses
... 30-kDa protein (p30) of tobacco mosaic virus (TMV), which was shown to affect viral host range to be essential for virus movement (15, 18), to be localized to plasmodesmata (16, 17, 34, 42, 50), and to alter size ...
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Identification by Phage Display and Characterization of Two Neutralizing Chimpanzee Monoclonal Antibodies to the Hepatitis E Virus Capsid Protein
... 55-kDa ORF2 protein of the SAR-55 strain that was used to vaccinate macaques against HEV elicited a humoral immune response that corre- lated with protection from hepatitis E, even when a high chal- lenge ...
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Antigenic Domains of the Open Reading Frame 2 Encoded Protein of Hepatitis E Virus
... (HEV) protein encoded by open reading frame 2 (ORF2) was determined by using synthetic ...entire ORF2 protein of the HEV Burma strain, were ...the ORF2 protein were ...the ...
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Hepatitis E Virus Genotype 1 Infection of Swine Kidney Cells In Vitro Is Inhibited at Multiple Levels
... HEV ORF2 protein has rou- tinely been used to identify cells either infected or transfected with HEV (12, ...of ORF2 protein. Separate immunostaining for ORF2 and ORF3 proteins detected ...
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A small highly basic protein is encoded in overlapping frame within the P gene of vesicular stomatitis virus.
... Analysis of the recently published sequence of the P gene of another distantly related vesiculovirus, Piry virus 3, also reveals the potential for an ORF2 protein encoded by bases 77 to [r] ...
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Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2
... ORF1-encoded protein, but there was no cross- reactivity between the ORF2-encoded proteins of PCV1 and PCV2 ...whole ORF2 in ...whole ORF2 protein was not expressed successfully in ...
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Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein
... HEV ORF2 protein release from Hep293TT cells electroporated with B6Nluc or B6Nluc_GAD full length RNA was measured by ...the ORF2 protein (F) or by luciferase assay ...
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Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins
... that ORF2 of MFSV encodes the P protein because (i) P is located adjacent to N in all rhabdoviruses sequenced so far, (ii) the predicted size of the ORF2 protein is similar to those of P ...
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Heparan Sulfate Proteoglycans Are Required for Cellular Binding of the Hepatitis E Virus ORF2 Capsid Protein and for Viral Infection
... HEV ORF2 protein binds to HSPGs on Huh-7 cells. The 56-kDa HEV ORF2 VLPs were found to attach as punc- tate structures all over and just outside the cellular membrane of Huh-7 cells ...607 ...
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The Lysine Residues within the Human Ribosomal Protein S17 Sequence Naturally Inserted into the Viral Nonstructural Protein of a Unique Strain of Hepatitis E Virus Are Important for Enhanced Virus Replication
... viral protein expression and infectivity. We utilized the RSV NC protein, which possesses nucleic acid binding, nuclear localization, and protein dimerization ...HEV protein expression levels ...
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Demonstration that orf2 Encodes the Feline Immunodeficiency Virus Transactivating (Tat) Protein and Characterization of a Unique Gene Product with Partial Rev Activity
... the orf2 gene coincides in size and location to the tat ORFs of the ungulate viruses (7, 10, ...FIV orf2 has been postulated to encode a transcrip- tional transactivator ...The orf2 genes of FIV-PPR ...
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A candidate DNA vaccine encoding a fusion protein of porcine complement C3d P28 and ORF2 of porcine circovirus type 2 induces cross protective immunity against PCV2b and PCV2d in pigs
... length ORF2 gene of PCV2 HeB-1 strain was amplified by using the primers 5′-ATCGCTAGCGCCGCCACCATGACGTATCCAA- 3′ and 5′-CCCAAGCTTTCACTTAGGGTTAAGT-3′, cut with Nhe I/Hind III and ligated into pVAX 1 yielding ...The ...
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