organotypic brain slice cultures
Original Article Implantation of glioblastoma spheroids into organotypic brain slice cultures as a model for investigating effects of irradiation: a proof of concept
... malignant brain tumor with an overall survival of only ...and brain tissue are highly needed in order to develop novel efficient therapeutic ...rat brain slice culture model comprising both ...
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Organotypic brain slice cultures to model neurodegenerative proteinopathies
... propagates in AD and the mechanisms behind this propagation [57, 64] and these are questions which could be explored in BSC models. The relationship be- tween tau and neurodegeneration is likely to be critical in our ...
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HIV-1 Tat activates indoleamine 2,3 dioxygenase in murine organotypic hippocampal slice cultures in a p38 mitogen-activated protein kinase-dependent manner
... IFNg is considered to be the prototypical inducer of IDO in a variety of cells [23] as well as in clinical situa- tions in which inflammation-associated depression occurs [45]. Additionally, IFNg is increased in the ...
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An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors
... All animal procedures were approved by the OHSU Institutional Animal Care and Use Committee (IACUC) according to the NIH Guide for the Care and Use of Laboratory Animals. Timed pregnant Sprague-Dawley (SD) rats were ...
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The dual S1PR1/S1PR5 drug BAF312 (Siponimod) attenuates demyelination in organotypic slice cultures
... The clinical success of pFTY720 in the treatment of relapsing remitting MS demonstrated the therapeutic potential of S1PR modulation and prompted efforts to develop more specific agonists for these receptors. Mod- ...
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Synaptophysin depletion and intraneuronal Aβ in organotypic hippocampal slice cultures from huAPP transgenic mice
... whole brain slices from adult APP_SDI mice; a failure to develop plaques, but appearance of atypical intraneur- onal Aβ staining ...in slice culture is more prone to retain Aβ within cells than in vivo so ...
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Central Administration of Lipopolysaccharide Induces Depressive-like Behavior in Vivo and Activates Brain Indoleamine 2,3 Dioxygenase In Murine Organotypic Hippocampal Slice Cultures
... this brain area in major depressive disorders [27,47,48] but by the need to compare ex vivo data to in situ data collected in ...of brain cells, OHSCs have the advantage of con- serving some form of ...
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Neuronal uptake of anti-Hu antibody, but not anti-Ri antibody, leads to cell death in brain slice cultures
... however, the role of antibodies to the HuD protein in the pathogenesis of neuronal death has not been estab- lished [15]. To determine whether antibodies reactive with the HuD protein were required for neuronal cyto- ...
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Borna Disease Virus Replication in Organotypic Hippocampal Slice Cultures from Rats Results in Selective Damage of Dentate Granule Cells
... whether organotypic hippocampal slice cultures from newborn rats could provide a suitable system to study BDV- induced pathology in this brain ...such slice cultures resulted in ...
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Dexmedetomidine is neuroprotective in an in vitro model for traumatic brain injury
... for organotypic slice cultures because this model allows easy access to in vitro manipulation of ner- vous tissue and yet mimics closely the in vivo state of the tissue with respect to morphological ...
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Inflammatory and cytotoxic effects of bifenthrin in primary microglia and organotypic hippocampal slice cultures
... SPs, including bifenthrin (BF), have been found to in- duce oxidative stress in animal models resulting in both increased oxidant markers and decreased antioxidant activities [12, 13]. BF is a type I pyrethroid ...
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Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro
... from organotypic brain slices and transverse spinal cord ...notypic brain slice cultures. Organotypic brain slices were embedded in a three-dimensional collagen matrix in ...
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Role of the E1∧E4 Protein in the Differentiation-Dependent Life Cycle of Human Papillomavirus Type 31
... Keratinocytes that maintain E1 ∧ E4 mutant genomes are impaired in differentiation-dependent amplification. We next examined if the loss of E1 ∧ E4 function had an effect on the differentiation-dependent amplification of ...
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Organotypic slice culture model demonstrates inter-neuronal spreading of alpha-synuclein aggregates
... The hypothesis of trans-synaptic spreading in the slice model was corroborated by the fact that cutting the axons in the CA3 region blocked spreading to regions distal to the lesion. To demonstrate that the ...
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Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
... Neuroinflammation is a common process in several neurological diseases, including MS where it leads to demyelination and axonal damage. To assess how methylthioadenosine might affect demyelination/remyeli- nation under ...
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Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injury
... In cultures that were previously stimulated (with differentiation growth factors in the case of macrophages and conditioned medium in the case of astrocytes), cells underwent several medium changes over 24 h ...
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FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel
... in cultures of the early postnatal mouse cochlea are more than twice as large as those recorded in inner hair cells (Kros et ...mutant cultures, which can transduce but are known to have all channels closed ...
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Jaagsiekte sheep retrovirus infection of lung slice cultures
... lung slice model described here provides an ideal plat- form for future studies to dissect the temporal changes in pathway signaling and to examine other aspects of JSRV infection and ...
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Squamous epitheliotropism of Enterovirus A71 in human epidermis and oral mucosa
... these samples. Moreover, PCR has been increasingly used for viral detection and reported as either positive or negative. Our data provides an indication that viral replication in epidermal keratinocytes is very active ...
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Three-dimensional tumor cell cultures employed in virotherapy research
... solution. Organotypic tissue cultures derived from primary tumor tissue maintain the histologi- cal cell architecture of the tumor, and can be employed as safety assays for OVs as ...raft cultures ...
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