organotypic culture
Src-mediated morphology transition of lung cancer cells in three-dimensional organotypic culture
... three-dimensional organotypic culture (rBM 3-D) to investigate the mechanisms that mediate the tumor promoting effects of the fibrogenic mediators TGF- β 1 and type I collagen (Col-1) on lung adenocarcinoma ...
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Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System.
... In order to specifically study the capacity of CAFs to form tracks in the gel that allow subsequent cancer cell invasion, as well as the potential of cancer cells to invade a gel previously remodeled by CAFs, a variation ...
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Modeling inflammation and oxidative stress in gastrointestinal disease development using novel organotypic culture systems
... (3D) organotypic culture (OTC) methods utilize epithelial cells grown on top of collagen/Matrigel matrices containing human fetal eso- phageal fi broblasts ...this culture system to model human ...
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Induction of HOXA9 expression in three-dimensional organotypic culture of the Claudin-low breast cancer cells
... three-dimensional organotypic culture (lrECM ...3D culture of the Claudin-low breast cancer cells MDA-MB-231 and ...3D culture required binding of bromodomain containing 4 to the HOXA9 ...
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Organotypic culture of acinar cells for the study of pancreatic cancer initiation
... 3D culture of acinar cells are the difficulty to extend the culture for a period of time long enough to allow a long-term experiment and the significant stress and variability introduced by isolation ...
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Detection of nanolevel drug metabolites in an organotypic culture of primary human hepatocytes and porcine hepatocytes with special reference to a two-compartment model
... Abstract: The quantification of drug metabolites produced during drug metabolism is a growing concern for the pharmaceutical industry, regulatory agencies such as the US Food and Drug Administration, the European ...
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Human conjunctival epithelial precursor cells and their progeny in 3D organotypic culture
... p63 expression (Fig. 1A) was exclusively confined to the nucleus of cells of the basal layer of the conjunctiva. 5% ± 2.2 % of basal epithelial cells were cycling, (positive immunostaining for Ki-67, Fig. 1B). ...
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Regeneration of TiO2 Nanotube Arrays after Long-Term Cell and Tissue Culture for Multiple Use – an Environmental Scanning Electron Microscopy (ESEM) Survey of Adult Pig Retina and beyond
... long-term culture of guinea pig adult retinal tissue is one of the key achievements that became feas- ible by introduction of nanotube substrates as reported by us previously ...conventional culture methods ...
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Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure
... EHP-induced RGC death provided critical evidence that the death of RGCs upon exposure to EHP or LPS in ret- inal organotypic cultures actually resulted from the impact of pro-inflammatory cytokines. This is in ...
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Human prostate supports more efficient replication of HIV 1 R5 than X4 strains ex vivo
... Characterization of human prostate in organotypic culture. Paraformaldehyde-fixed paraffin sections were examined morphologically and immunostained for several prostatic cell markers before culture ...
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Methylthioadenosine promotes remyelination by inducing oligodendrocyte differentiation
... A Organotypic cultures were treated with MTA (192 μ M) or the placebo for 24 h at seven DIV and thereafter, they were stimulated with LPS (15 μ g/ ml) for 24 ...Cerebellar organotypic cultures at seven DIV ...
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The metastatic infiltration at the metastasis/brain parenchyma- interface is very heterogeneous and has a significant impact on survival in a prospective study
... Despite this growing body of evidence demon- strating the presence of metastatic infiltration beyond the glial pseudo-capsule and its potential clinical implications, the result of the above mentioned prospective biopsy ...
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Changes in Na+/K+ ATPase expression during adaptive cell differentiation in avian nasal salt gland
... Germany) was added to the culture medium for 1 or 3 h. Slices were washed in fresh medium and homogenized in a Hepes- buffered saline (Shuttleworth and Thompson, 1989) containing 50 µmol l −1 Pefablock (Roth, ...
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Molecular interplay between leptin, insulin-like growth factor-1, and β-amyloid in organotypic slices from rabbit hippocampus
... the organotypic slices using a quantitative sandwich ELISA kit (R & D systems, Minneapolis, MN) as per the manufacturer ’ s ...protocol. Organotypic slices were homogenized in T-PER tissue protein ...
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A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
... First, we wanted to know how well hippocampal slices would recover from the trauma caused by the isolation procedure. Therefore, we collected the medium after every 24 h during 7 DIV and used standardised protocols to ...
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Human Papillomavirus Type 8 E6 Oncoprotein Inhibits Transcription of the PDZ Protein Syntenin-2
... 6-cm culture dishes with expression vectors for Flag- p53 or Flag–syntenin-2 and HPV16 E6-Flag expression vectors, and p53 or syntenin-2 levels were detected by Western ...
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Organotypic brain slice cultures to model neurodegenerative proteinopathies
... as, effects of γ-secretase inhibition [44]. Overall, these BSC models have shown subtle Aβ immunoreactivity (small diffuse deposits, limited/no Thioflavin S positivity, absence of dystrophic neurites, limited/no gliosis) ...
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Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system
... in organotypic cocultures is delayed and require an increased number of fibroblasts ...In organotypic cocultures of HaCaT cells either with human or mouse fibroblasts well structured and differentiated ...
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Organotypic in vitro models of human cutaneous squamous cell carcinoma
... weeks culture, the epidermis generated on rat‐tail collagen‐based HSEswas reduced very fast until only 3–4 cell layers were left in the eighth week, while the epidermis generated on FDM‐based HSEs maintained its ...
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An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors
... of organotypic slice cultures for studies of OL biology and myelination due to retention of multi-cellular inter- actions and the ease of manipulation ...that organotypic slice cultures display features ...
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